scholarly journals Correlation of cytopathic effect, fluorescent-antibody microneutralization, and plaque reduction test results for determining avian infectious bronchitis virus antibodies

1977 ◽  
Vol 5 (3) ◽  
pp. 361-364
Author(s):  
R E Wooley ◽  
J Brown
Keyword(s):  
Infectious Bronchitis Virus ◽  
Cytopathic Effect ◽  
Fluorescent Antibody ◽  
Avian Infectious Bronchitis Virus ◽  
Test Results ◽  
Infectious Bronchitis ◽  
Large Numbers ◽  
Significant Difference ◽  
Positive Correlations ◽  
Time Required

A microneutralization fluorescent-antibody (MFA) test was effective in determining the level of antibodies to avian infectious bronchitis virus. A comparison of the MFA test with the cytopathic effect microneutralization (MNT) test and 50% plaque reduction (PR) test resulted in positive correlations that were significant (P less than 0.001). The PR test was more sensitive than either the MFA or the MNT test, but there was no significant difference between the sensitivities of the MFA and MNT tests. The MFA test has advantages over the PR test in the capacity to test large numbers of sera in a shorter period of time. The MFA test also can be completed in one-half the time required for the MNT test.

scholarly journals Identification and Analysis of Long Noncoding RNAs and mRNAs in Chicken Macrophages Infected With Coronavirus Avian Infectious Bronchitis Virus

2020 ◽  
Author(s):  
Hao Li ◽  
Pengfei Cui ◽  
Xue Fu ◽  
Lan Zhang ◽  
Wenjun Yan ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Acid Metabolism ◽  
Expression Profiles ◽  
Nucleic Acid Metabolism ◽  
Avian Infectious Bronchitis Virus ◽  
Rna Seq ◽  
Data Bases ◽  
Infectious Bronchitis ◽  
Significant Difference ◽  
Non Coding Rnas

Abstract Background: Avian infectious bronchitis virus (IBV) is a gammacoronavirus that seriously affects the world's poultry industry Long non coding RNAs (lncRNAs), a subset of non coding RNAs greater than 200 nucleotides in length, have been recently recognized as pivotal factors during the pathogenesis of viral infection. However, how lncRNAs in host cul tured cells respond to IBV infection was little known. Herein, we detected the expression profiles of the mRNAs and lncRNAs in IBV infected HD11 cells. Results: By RNA seq, 2640 novel lncRNAs were identified, and 181 lncRNAs (59 up regulated lncRNAs , 122 down regulated lncRNAs) exhibited significant difference in expression levels in IBV infected HD11 cells compared with the uninfected. Based on the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) data bases, the significant diff erentially expressed (DE) lncRNAs, such as MSTRG.25416.43, MSTRG.6458.31, MSTRG.14220.1 and MSTRG.21445.2, were mainly involved in the regulation of cellular innate immunity and amino acid, nucleic acid metabolism. In addition, 30 DE lncRNAs were screened out, and these lncRNAs may interact with gga miR 30d to regulate IBV replication. Conclusions:Our results provided novel insights into the functions of lncRNAs and the possible pathogenic mechanism following IBV infection.

scholarly journals Plastic multiwell plates to assay avian infectious bronchitis virus in organ cultures of chicken embryo trachea

1978 ◽  
Vol 8 (4) ◽  
pp. 380-387
Author(s):  
S Yachida ◽  
S Aoyama ◽  
N Takahashi ◽  
Y Iritani ◽  
K Katagiri
Keyword(s):  
Infectious Bronchitis Virus ◽  
Chicken Embryo ◽  
Tracheal Ring ◽  
Avian Infectious Bronchitis Virus ◽  
Organ Cultures ◽  
Infectious Bronchitis ◽  
Eagle Minimal Essential Medium ◽  
The Mean ◽  
Ethanesulfonic Acid ◽  
Time Required

Simple assay systems for infectivity titrations of avian infectious bronchitis virus (IBV) in chicken embryo trachea organ cultures (OC) were developed using plastic multiplate wells with one tracheal ring per well; these assays appeared to be much more satisfactory than the conventional rolled-tube method. The medium, 0.05 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered Eagle minimal essential medium was not changed during observation. A medium containing 0.4% bovine serum albumin did not influence the virus yield, but did stabilize virus viability during storage. Reproducibility of results obtained in the OC system was confirmed by performing replicate titrations of the Beaudette strain with three different passage histories. The mean virus titers in the OC were lower than those in chicken embryos, depending on the IBV passage histories. The time required for ciliostasis was related not only to the concentration of virus, but also to the IBV passage history. Application of OC techniques for the constant serum-variable virus neutralization test gave low neutralization indexes with excellent reproducibility as compared with those obtained in the chicken embryo assay system. Also, the slopes of neutralization curves obtained by assays in OC were less steep than those seen in the chicken embryo system.

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

Avian infectious bronchitis virus: isolation of an apparently new variant in Italy

2000 ◽  
Vol 146 (7) ◽  
pp. 191-193 ◽  
Author(s):  
A. Zanella ◽  
R. Coaro ◽  
R. Marchi ◽  
G. Fabris ◽  
A. Lavazza
Keyword(s):  
Infectious Bronchitis Virus ◽  
Virus Isolation ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
New Variant

scholarly journals Research Note: Antibodies to Avian Infectious Bronchitis Virus in Pakistani Chickens

1987 ◽  
Vol 66 (4) ◽  
pp. 765-767 ◽  
Author(s):  
MOHAMMAD A. MUNEER ◽  
JOHN A. NEWMAN ◽  
SAGAR M. GOYAL ◽  
M. AJMAL
Keyword(s):  
Infectious Bronchitis Virus ◽  
Research Note ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis
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