scholarly journals Genetic Analysis of Israel Acute Paralysis Virus: Distinct Clusters Are Circulating in the United States

2008 ◽  
Vol 82 (13) ◽  
pp. 6209-6217 ◽  
Author(s):  
G. Palacios ◽  
J. Hui ◽  
P. L. Quan ◽  
A. Kalkstein ◽  
K. S. Honkavuori ◽  
...  

ABSTRACT Israel acute paralysis virus (IAPV) is associated with colony collapse disorder of honey bees. Nonetheless, its role in the pathogenesis of the disorder and its geographic distribution are unclear. Here, we report phylogenetic analysis of IAPV obtained from bees in the United States, Canada, Australia, and Israel and the establishment of diagnostic real-time PCR assays for IAPV detection. Our data indicate the existence of at least three distinct IAPV lineages, two of them circulating in the United States. Analysis of representatives from each proposed lineage suggested the possibility of recombination events and revealed differences in coding sequences that may have implications for virulence.

2017 ◽  
Vol 7 (2) ◽  
pp. 1050-1056 ◽  
Author(s):  
Jonathan Farley

The disappearance of honey bees from many managed colonies in the United States and Europe in 2006 and 2007 is modeled under the assumption that the cause is some contagion.  Based on the limited data available, we use a simple model to suggest that colony collapse disorder will not destroy all colonies in the United States.  To predict the evolution of future outbreaks, however, and perhaps trace their origins, it is recommended that graph-theoretic data be collected, and that census data be collected on a more frequent basis, concerning bee populations. 


Author(s):  
Sainath Suryanarayanan

On your next stroll outdoors, you may come across a flowering plant, enjoy its beauty, and perhaps even taste its fruits. A wandering Homo sapiens, however, is probably not the flowering plant’s primary audience; an insect pollinator is more likely the one being wooed. Indeed, the vast biodiversity of flowering plants and insects on Earth is thought to be the result of a fruitful co-evolution over several million years between these organisms (Price 1997, pp. 239–258). Bees, wasps, butterflies, flies, and several other insects are also crucial in their role as pollinators for sus­taining managed agricultural ecosystems (or agro-ecosystems; National Research Council [NRC] 2007). Honey bees (Apis mellifera), managed by beekeepers, are alone estimated to be responsible for over $15 billion worth of increased yield and quality in the United States annually (Morse and Calderone 2000). U.S. growers rent an estimated 2 million beehives each year from beekeepers to pollinate over ninety different fruit, vegetable, and fiber crops (Delaplane and Mayer 2000; NRC 2007). In the first decades of the 21st century, public and scientific attention in the United States and elsewhere has been gripped by frequent reports of declines in populations of insect pollinators (e.g., Biesmeijer et al. 2006; NRC 2007), exemplified most dramatically by the news of Colony Collapse Disorder (CCD) among managed honey bees (vanEngelsdorp et al. 2009; Pettis and Delaplane 2010). While there are ongoing scientific and public debates over the extent to which the documented declines in insect pollinators constitute a global “pollinator crisis,” whether agricultural productivity has actually declined due to these losses, and what the primary causal factors are, there is nonetheless a consensus that parts of North America and Europe continue to undergo worrying reductions in the diversity and abundance of multiple species of insect pollinators (Ghazoul 2005; Stefan-Dewenter et al. 2005; NRC 2007; Carvalheiro et al. 2013). In this chapter, I analyze the main kinds of efforts that are being taken by key institutional players to resolve the environmental problem of pollinator decline in the United States.


2020 ◽  
Vol 9 (4) ◽  
Author(s):  
Mohammad Shamim Ahasan ◽  
William Keleher ◽  
Cem Giray ◽  
Brenda Perry ◽  
Win Surachetpong ◽  
...  

Here, we present the complete coding sequences of two tilapia lake virus (TiLV) isolates recovered during an investigation of a mortality event in farmed Nile tilapia in the United States. Phylogenetic analysis supported the isolates as each other’s closest relatives and members of a clade of Thai TiLV strains.


Author(s):  
Denise de Oliveira Scoaris ◽  
Frederic Mendes Hughes ◽  
Milton Adolfo Silveira ◽  
Jay Daniel Evans ◽  
Jeffery Stuart Pettis ◽  
...  

PEDIATRICS ◽  
1994 ◽  
Vol 94 (1) ◽  
pp. 118-118
Author(s):  
J. F. L.

Harlington, TX, July 19 (AP)—Federal agricultural officials say that the honey bees that killed an 82-year-old rancher last week were the Africanized variety known as "killer bees." "Our lab has confirmed that the bees are Africanized," said Kim Kaplan, a Spokeswoman for the Federal Department of Agriculture in Greenbelt, MD. Final autopsy results are not yet available, but the pathologist who did the autopsy listed the preliminary cause of death as acute fluid buildup in the lungs caused by insect stings. If the cause of death is confirmed, the rancher, Lino Lopex, would be the first person killed by Africanized bees in the United States since the aggressive variety migrated into Texas in 1990. Harlington, TX, in South Texas, is about 15 miles from the Mexican border. Mr. Lopez apparently tried to drive the bees out of a wall in an abandoned house by poking the hive with a stick wrapped with a burning burlap sack. He was dead on arrival at the hospital, with about 40 stingers still attached to his body, officials said.


2019 ◽  
Vol 6 (7) ◽  
Author(s):  
Ayesha Khan ◽  
Truc T Tran ◽  
Rafael Rios ◽  
Blake Hanson ◽  
William C Shropshire ◽  
...  

Abstract Background Treatment of serious infections due to multidrug-resistant (MDR) Pseudomonas aeruginosa remains a challenge, despite the introduction of novel therapeutics. In this study, we report 2 extensively drug-resistant clinical isolates of sequence type (ST) 309 P aeruginosa resistant to all β-lactams, including the novel combinations ceftolozane/tazobactam, ceftazidime/avibactam, and meropenem/vaborbactam. Methods Isolates were sequenced using both short-read (Illumina) and long-read technology to identify resistance determinants, polymorphisms (compared with P aeruginosa PAO1), and reconstruct a phylogenetic tree. A pair of β-lactamases, Guiana extended spectrum β-lactamase (GES)-19 and GES-26, were cloned and expressed in a laboratory strain of Escherichia coli to examine their relative impact on resistance. Using cell lysates from E coli expressing the GES genes individually and in tandem, we determined relative rates of hydrolysis for nitrocefin and ceftazidime. Results Two ST309 P aeruginosa clinical isolates were found to harbor the extended spectrum β-lactamases GES-19 and GES-26 clustered in tandem on a chromosomal class 1 integron. The presence of both enzymes in E coli was associated with significantly elevated minimum inhibitory concentrations to aztreonam, cefepime, meropenem, ceftazidime/avibactam, and ceftolozane/tazobactam, compared with those expressed individually. The combination of ceftazidime/avibactam plus aztreonam was active in vitro and used to achieve cure in one patient. Phylogenetic analysis revealed ST309 P aeruginosa are closely related to MDR strains from Mexico also carrying tandem GES. Conclusions The presence of tandem GES-19 and GES-26 is associated with resistance to all β-lactams, including ceftolozane/tazobactam. Phylogenetic analysis suggests that ST309 P aeruginosa may be an emerging threat in the United States.


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