Cell-Based Quantification of Chronic Wasting Disease Prions
Keyword(s):
ABSTRACT Cell-based measurement of prion infectivity is currently restricted to experimental strains of mouse-adapted scrapie. Having isolated cell cultures with susceptibility to prions from diseased elk, we describe a modification of the scrapie cell assay allowing evaluation of prions causing chronic wasting disease, a naturally occurring transmissible spongiform encephalopathy. We compare this cervid prion cell assay to bioassays in transgenic mice, the only other existing method for quantification, and show this assay to be a relatively economical and expedient alternative that will likely facilitate studies of this important prion disease.
2003 ◽
Vol 15
(3)
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pp. 274-277
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2006 ◽
Vol 80
(2)
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pp. 596-604
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2016 ◽
Vol 54
(4)
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pp. 1108-1116
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