Silencing of both β-TrCP1 and HOS (β-TrCP2) Is Required To Suppress Human Immunodeficiency Virus Type 1 Vpu-Mediated CD4 Down-Modulation
Keyword(s):
ABSTRACT The human immunodeficiency virus type 1 (HIV-1) Vpu protein interacts with CD4 within the endoplasmic reticula of infected cells and targets CD4 for degradation through interaction with β-TrCP1. Mammals possess a homologue of β-TrCP1, HOS, which is also named β-TrCP2. We show by coimmunoprecipitation experiments that β-TrCP2 binds Vpu and is able to induce CD4 down-modulation as efficiently as β-TrCP1. In two different cell lines, HeLa CD4+ and Jurkat, Vpu-mediated CD4 down-modulation could not be reversed through the individual silencing of endogenous β-TrCP1 or β-TrCP2 but instead required the two genes to be silenced simultaneously.
1994 ◽
Vol 180
(4)
◽
pp. 1283-1293
◽
2002 ◽
Vol 76
(3)
◽
pp. 1015-1024
◽
2001 ◽
Vol 75
(16)
◽
pp. 7266-7279
◽
2000 ◽
Vol 74
(11)
◽
pp. 5373-5376
◽
2005 ◽
Vol 79
(21)
◽
pp. 13579-13586
◽