Localization of Three Major Capped 5′ Ends of Polyoma Virus Late mRNA's Within a Single Tetranucleotide Sequence in the Viral Genome †

An unidentified virus, responsible for an arthritic-like condition in chickens was studied by electron microscopy and other methods of viral investigation. It was characterized in chorio-allantoic membrane (CAM) lesions of embryonating chicken eggs and in tissue culture as to: 1) particle size; 2) structure; 3) mode of replication in the cell; and 4) nucleic acid type.The inoculated virus, coated and uncoated, is first seen in lysosomal-like inclusions near the nucleus; the virions appear to be uncoated in these electron dense inclusions (Figure 1), Although transfer of the viral genome from these inclusions is not observable, replicating virus and mature virus crystals are seen in the cytoplasm subsequent to the uncoating of the virions.The crystals are formed in association with a mass of fibrils 50 to 80 angstroms in diameter and a ribosome-studded structure that appears to be granular endoplasmic reticulum adapted to virus replication (Figure 2). The mature virion (Figure 3) is an icosahedral particle approximately 75 millimicrons in diameter. The inner core is 45 millimicrons, the outer coat 15 millimicrons, and the virion has no envelope.

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604: Polyoma Virus Infection as an Early Marker for Bladder Transitional Cell Carcinoma

The Journal of Urology ◽

10.1016/s0022-5347(18)34844-4 ◽

2005 ◽

Vol 173 (4S) ◽

pp. 165-165

Author(s):

Paolo Gontero ◽

Elisabetta Omodeo-Zorini ◽

Paola Cacciotti ◽

Filippo Sogni ◽

Ervin Kocjancic ◽

...

Keyword(s):

Virus Infection ◽

Transitional Cell Carcinoma ◽

Cell Carcinoma ◽

Transitional Cell ◽

Polyoma Virus ◽

Bladder Transitional Cell Carcinoma ◽

Early Marker

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DETECTION OF ALFACORONAVIRUSES, BETACORONAVIRUSES AND ASTROVIRUSES IN BAT FECAL SAMPLES FROM MOSCOW REGION

Molecular Diagnostics and Biosafety – 2020. Russian national scientific and practical conference with international participation (October, 6–8, 2020): Conference Proceedings ◽

10.36233/978-5-9900432-9-9-42 ◽

2020 ◽

Author(s):

E.V. Korneenko ◽

◽

А.E. Samoilov ◽

I.V. Artyushin ◽

M.V. Safonova ◽

...

Keyword(s):

High Throughput ◽

Viral Genome ◽

High Throughput Sequencing ◽

Pcr Amplification ◽

Moscow Region ◽

Fecal Samples ◽

Genus Level ◽

Zoonotic Viruses ◽

Reservoir Hosts ◽

Blastn Search

In our study we analyzed viral RNA in bat fecal samples from Moscow region (Zvenigorod district) collected in 2015. To detect various virus families and genera in bat fecal samples we used PCR amplification of viral genome fragments, followed by high-throughput sequencing. Blastn search of unassembled reads revealed the presence of viruses from families Astroviridae, Coronaviridae and Herpesviridae. Assembly using SPAdes 3.14 yields contigs of length 460–530 b.p. which correspond to genome fragments of Coronaviridae and Astroviridae. The taxonomy of coronaviruses has been determined to the genus level. We also showed that one bat can be a reservoir of several virus genuses. Thus, the bats in the Moscow region were confirmed as reservoir hosts for potentially zoonotic viruses.

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Prevalent and diverse intratumoral oncoprotein-specific CD8+ T cells within polyoma virus-driven Merkel cell carcinomas

immuneACCESS ◽

10.21417/lj2019cir ◽

2020 ◽

Author(s):

L Jing ◽

M Ott ◽

CD Church ◽

RM Kulikauskas ◽

D Ibrani ◽

...

Keyword(s):

T Cells ◽

Cd8 T Cells ◽

Polyoma Virus ◽

Merkel Cell

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