Structure and restriction enzyme maps of the circularly permuted DNA of staphylococcal bacteriophage phi 11.

1981 ◽  
Vol 37 (2) ◽  
pp. 784-794 ◽  
Author(s):  
S Löfdahl ◽  
J Zabielski ◽  
L Philipson
Keyword(s):  
Restriction Enzyme ◽  
Restriction Enzyme Maps

scholarly journals Molecular analysis of the Coprinus cinereus mating type A factor demonstrates an unexpectedly complex structure.

Genetics ◽  
1991 ◽  
Vol 128 (3) ◽  
pp. 529-538 ◽  
Author(s):  
G May ◽  
L Le Chevanton ◽  
P J Pukkila
Keyword(s):  
Mating Type ◽  
Restriction Enzyme ◽  
Complex Structure ◽  
Coprinus Cinereus ◽  
Genetic Studies ◽  
Basidiomycetous Fungus ◽  
Dominant Phenotype ◽  
Enzyme Mapping ◽  
Type Factor ◽  
Restriction Enzyme Maps

Abstract We report here the molecular cloning of the A43 mating type factor from Coprinus cinereus, a basidiomycetous fungus. Our molecular analyses revealed an unexpected source of variation in the A factor. Though genetic studies have demonstrated that A has two subunits, alpha and beta, we located three nonoverlapping fragments in the A43 region that have A factor function following DNA-mediated transformation. The three fragments demonstrate no similarity to one another as judged by restriction enzyme maps and by hybridization on Southern blots. We conclude that the A43 factor is composed of at least three subunits. When strains carrying different A factors are examined by hybridization to the cloned subunits, extensive polymorphism is seen. Both intensity of hybridization and restriction fragment lengths vary between strains. Some strains fail to show any hybridization to a probe. In contrast, other strains from widely separated geographic locations apparently share very similar subunits. From comparative restriction enzyme mapping of A43 and a mutated A43 factor, we inferred that a 12-kb deletion in the A factor was responsible for the constitutive, dominant phenotype of the mutated A factor. The results of transformation experiments support an activator model for the activity of the A factor in regulating the A pathway.

Restriction enzyme maps for equine adenovirus 1 genome

1992 ◽  
Vol 31 (4) ◽  
pp. 343-349 ◽  
Author(s):  
Michael Sheppard ◽  
Sarah M. Drysdale ◽  
Michael J. Studdert
Keyword(s):  
Restriction Enzyme ◽  
Restriction Enzyme Maps

scholarly journals XbaI, PstI, and BglII restriction enzyme maps of the two orientations of the varicella-zoster virus genome.

1981 ◽  
Vol 39 (2) ◽  
pp. 390-400 ◽  
Author(s):  
A M Dumas ◽  
J L Geelen ◽  
M W Weststrate ◽  
P Wertheim ◽  
J van der Noordaa
Keyword(s):  
Varicella Zoster Virus ◽  
Restriction Enzyme ◽  
Virus Genome ◽  
Varicella Zoster ◽  
Restriction Enzyme Maps

scholarly journals Structure of the c-Ki-ras gene in a rat fibrosarcoma induced by 1,8-dinitropyrene.

1986 ◽  
Vol 6 (4) ◽  
pp. 1349-1351 ◽  
Author(s):  
T Tahira ◽  
K Hayashi ◽  
M Ochiai ◽  
N Tsuchida ◽  
M Nagao ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Restriction Enzyme ◽  
Nucleotide Sequence Analysis ◽  
Ras Gene ◽  
Normal Rat ◽  
Restriction Enzyme Maps

Restriction enzyme maps were made of the region around exons 1 and 2 of activated c-Ki-ras of a fibrosarcoma (1,8-DNP2) induced in a rat by 1,8-dinitropyrene. Nucleotide sequence analysis revealed that activated c-Ki-ras shows a G----T transversion in codon 12 and consequently encodes cysteine instead of glycine in normal rat c-Ki-ras.

Restriction enzyme maps of the chimeric R/Ent plasmid pCG86 and the related Ent plasmid P307

Plasmid ◽  
1984 ◽  
Vol 11 (1) ◽  
pp. 102-104 ◽  
Author(s):  
Roger N. Picken ◽  
Anthony J. Mazaitis ◽  
Werner K. Maas
Keyword(s):  
Restriction Enzyme ◽  
Restriction Enzyme Maps
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