Human herpesvirus 6 infects cervical epithelial cells and transactivates human papillomavirus gene expression.

The purpose of our study was to confirm reports of an association of human papillomavirus (HPV) with neonatal giant cell hepatitis (GCH) and biliary atresia (BA), and to expand these studies to include cytomegalovirus (CMV), Epstein-Barr virus (EBV), human herpesvirus 6 (HHV6), and parvovirus B19 (PVB19). Frozen hepatic tissue was available for polymerase chain reaction (PCR) analysis in 19 cases of GCH or BA and 8 controls. Nested PCR to detect HPV types 6, 16, 18, and 33 was followed by 32P hybridization with generic probes. PCR followed by hybridization with a digoxigenin-labeled probe was used for all other viruses. HPV, EBV, and PVB19 were not detected in cases or controls. Two cases of GCH and 1 case of BA were PCR positive for CMV; controls were negative. HHV6 was detected in 6 cases: 2 GCH, 2 BA, and 2 controls. We conclude that HPV is not associated with GCH or BA. Detection of CMV in BA and GCH confirms other reports of this association. HHV6 requires further study to determine the significance of a positive PCR test in the livers of infants.

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Associations between human herpesvirus-6, human papillomavirus and cervical cancer

Cancer Letters ◽

10.1016/j.canlet.2013.04.023 ◽

2013 ◽

Vol 336 (1) ◽

pp. 18-23 ◽

Cited By ~ 6

Author(s):

E. Susan Amirian ◽

Karen Adler-Storthz ◽

Michael E. Scheurer

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Human Herpesvirus ◽

Human Herpesvirus 6 ◽

Herpesvirus 6

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Rare association of human herpesvirus 6 DNA with human papillomavirus DNA in cervical smears of women with normal and abnormal cytologies.

Journal of Clinical Microbiology ◽

10.1128/jcm.34.6.1589-1591.1996 ◽

1996 ◽

Vol 34 (6) ◽

pp. 1589-1591 ◽

Cited By ~ 10

Author(s):

N Romano ◽

F M Romano ◽

E Viviano ◽

F Vitale ◽

M R Villafrate ◽

...

Keyword(s):

Human Papillomavirus ◽

Human Herpesvirus ◽

Human Herpesvirus 6 ◽

Rare Association ◽

Cervical Smears ◽

Herpesvirus 6 ◽

Papillomavirus Dna

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Possible Involvement of Human Herpesvirus-6 U83 Gene Expression in Autoimmune Thyroiditis Development

Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences ◽

10.2478/prolas-2019-0013 ◽

2019 ◽

Vol 73 (2) ◽

pp. 78-83

Author(s):

Alina Sultanova ◽

Maksims Čistjakovs ◽

Lība Sokolovska ◽

Egils Cunskis ◽

Modra Murovska

Keyword(s):

Gene Expression ◽

Thyroid Gland ◽

Autoimmune Thyroiditis ◽

Blood Donors ◽

Human Herpesvirus ◽

Control Group ◽

Human Herpesvirus 6 ◽

Tissue Samples ◽

Cytokine Levels ◽

Herpesvirus 6

Abstract Viral infections have been frequently cited as important environmental factors implicated in autoimmune thyroiditis (AIT) development, although no specific virus has yet been conclusively associated with the disease. Some evidence implicates human herpesvirus-6 (HHV-6) in this disease. The aim of this study was to investigate the role of the HHV-6 U83 gene expression in autoimmune thyroiditis development. Fifty-one patients with AIT following thyroidectomy and a control group of 30 autopsied subjects without thyroid pathologies for comparing virology results and 30 healthy blood donors for comparing serology results were enrolled in this study. HHV-6 U83 gene expression was determined using nested PCR with complementary DNA as the template acquired from thyroid gland extracted RNA. Plasma samples of AIT patients and blood donors were tested for IL-2, IL-4, IL-10, sTNF-RII and IL-1beta levels by ELISA. Virology results were compared with pro- and anti-inflammatory cytokine levels to determine possible interaction of HHV-6 with host immune response. HHV-6 U83 gene expression was found only in 24% (12/49) of AIT patient thyroid gland tissue samples and in none of the control group individuals, showing possible involvement of this gene in AIT development. However, no interaction between HHV-6 and changes in cytokine levels was found.

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