scholarly journals Differential Epstein-Barr virus gene expression in B-cell subsets recovered from lymphomas in SCID mice after transplantation of human peripheral blood lymphocytes.

1995 ◽  
Vol 69 (1) ◽  
pp. 150-155 ◽  
Author(s):  
R Rochford ◽  
D E Mosier
Keyword(s):  
Gene Expression ◽  
B Cell ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Barr Virus ◽  
Human Peripheral Blood Lymphocytes ◽  
Epstein Barr ◽  
B Cell Subsets

scholarly journals Frequencies of the separate human B cell subsets activatable to Ig secretion by Epstein-Barr virus and pokeweed mitogen.

1983 ◽  
Vol 157 (6) ◽  
pp. 1808-1814 ◽  
Author(s):  
O Martínez-Maza ◽  
S Britton
Keyword(s):  
T Cells ◽  
B Cells ◽  
B Cell ◽  
Epstein Barr Virus ◽  
Human Peripheral Blood ◽  
Pokeweed Mitogen ◽  
Barr Virus ◽  
Human B Cells ◽  
Epstein Barr ◽  
B Cell Subsets

We have developed a microculture system suitable for limiting dilution analysis of Epstein-Barr virus (EBV)- and pokeweed mitogen (PWM)-induced activation of immunoglobulin secretion by human B cells. It was found that exogenous filler cells were not required to obtain optimal EBV-induced B cell precursor frequency (PF) estimates, although filler T cells were required for optimal PWM activation. In fact, when autologous T cells were used as filler cells, a marked decrease in the EBV-induced IgM PF was noted. Treatment of the T cells with cyclosporin A partially eliminated, and irradiation of the T cells completely eliminated, this decrease. The calculated PF of B cells activated by EBV was from 1/290 to 1/3,700 for IgM, and from 1/920 to 1/3,250 for IgG secretion. PWM activated from 1/140 to 1/3,200 B cells to IgM secretion. The results of experiments in which EBV and PWM were mixed, indicated that these two polyclonal activators operated on different B cell subpopulations. Therefore, both these agents seem to activate small, discrete subpopulations of human peripheral blood B cells to Ig secretion.

scholarly journals AID expression in peripheral blood of children living in a malaria holoendemic region is associated with changes in B cell subsets and Epstein-Barr virus

2014 ◽  
Vol 136 (6) ◽  
pp. 1371-1380 ◽  
Author(s):  
Joel R. Wilmore ◽  
Amolo S. Asito ◽  
Chungwen Wei ◽  
Erwan Piriou ◽  
P. Odada Sumba ◽  
...  
Keyword(s):  
B Cell ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
B Cell Subsets

A Quantitative Ultrastructural Study of Peripheral Blood Lymphocytes Containing Parallel Tubular Arrays in Epstein-Barr Virus and Cytomegalo-Virus Mononucleosis

1981 ◽  
Vol 39 ◽  
pp. 454-455
Author(s):  
C. M. Payne ◽  
P. M. Tennican
Keyword(s):  
Peripheral Blood ◽  
Lymphocyte Count ◽  
Epstein Barr Virus ◽  
Absolute Lymphocyte Count ◽  
Peripheral Blood Lymphocytes ◽  
Clinical State ◽  
Barr Virus ◽  
The Absolute ◽  
Epstein Barr ◽  
Tubular Arrays

In the normal peripheral circulation there exists a sub-population of lymphocytes which is ultrastructurally distinct. This lymphocyte is identified under the electron microscope by the presence of cytoplasmic microtubular-like inclusions called parallel tubular arrays (PTA) (Figure 1), and contains Fc-receptors for cytophilic antibody. In this study, lymphocytes containing PTA (PTA-lymphocytes) were quantitated from serial peripheral blood specimens obtained from two patients with Epstein -Barr Virus mononucleosis and two patients with cytomegalovirus mononucleosis. This data was then correlated with the clinical state of the patient.It was determined that both the percentage and absolute number of PTA- lymphocytes was highest during the acute phase of the illness. In follow-up specimens, three of the four patients' absolute lymphocyte count fell to within normal limits before the absolute PTA-lymphocyte count.In one patient who was followed for almost a year, the absolute PTA- lymphocyte count was consistently elevated (Figure 2). The estimation of absolute PTA-lymphocyte counts was determined to be valid after a morphometric analysis of the cellular areas occupied by PTA during the acute and convalescent phases of the disease revealed no statistical differences.

scholarly journals Inhibitory Effect of Epstein-Barr Virus Gene-Ebna1 on Human Tnfrp55 Gene Expression

2012 ◽  
Vol 56 (3) ◽  
pp. 271-274
Author(s):  
Joanna Krzowska-Firych ◽  
Hanna Fota-Markowska ◽  
Barbara Marzec-Kotarska ◽  
Roma Modrzewska ◽  
Jacek Wojcierowski
Keyword(s):  
Gene Expression ◽  
Epstein Barr Virus ◽  
Human Peripheral Blood ◽  
Rnase Protection Assay ◽  
Inhibitory Effect ◽  
Gene Encoding ◽  
Rnase Protection ◽  
Barr Virus ◽  
Epstein Barr ◽  
Ebna1 Gene

Abstract The aim of the study was to assess the expression of TNFRp55 mRNA and to examine if the antisense inhibition of Epstein-Barr virus (EBV) encoded EBNA1 gene product alters the expression of gene encoding TNFRp55 in lymphoblastoid cell line (LCL). The experiment was performed on LCL derived from EBV infected human peripheral blood B lymphocytes. The lymphocytes were isolated and cultured. RNA was isolated and examined according to the RNase protection assay. The hybridisation was done with HCR-4 probe. RNA was quantified by densitometry and presented in extinction units. The level of expression was calculated with TotaILab software programme. The results of the study suggest that EBV gene, responsible for the synthesis of EBNA1 protein, has an inhibitory effect on human TNFRp55 gene expression in LCL.

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