Expression of human endogenous retrovirus type K envelope glycoprotein in insect and mammalian cells.

1997 ◽  
Vol 71 (4) ◽  
pp. 2747-2756 ◽  
Author(s):  
R R Tönjes ◽  
C Limbach ◽  
R Löwer ◽  
R Kurth
Keyword(s):  
Envelope Glycoprotein ◽  
Mammalian Cells ◽  
Endogenous Retrovirus ◽  
Human Endogenous Retrovirus ◽  
Type K

scholarly journals Envelope Gene of the Human Endogenous Retrovirus HERV-W Encodes a Functional Retrovirus Envelope

2001 ◽  
Vol 75 (7) ◽  
pp. 3488-3489 ◽  
Author(s):  
Dong Sung An ◽  
Yi-ming Xie ◽  
Irvin S. Y. Chen
Keyword(s):  
Human Immunodeficiency Virus ◽  
Functional Properties ◽  
Envelope Glycoprotein ◽  
Endogenous Retrovirus ◽  
Human Endogenous Retrovirus ◽  
Envelope Gene ◽  
Membrane Glycoprotein ◽  
Herv Family ◽  
Immunodeficiency Virus

ABSTRACT A member of the human endogenous retrovirus (HERV) family termed HERV-W encodes a highly fusogenic membrane glycoprotein that appears to be expressed specifically in the placenta. It is unclear whether the glycoproteins of the HERVs can serve as functional retrovirus envelope proteins to confer infectivity on retrovirus particles. We found that the HERV-W envelope glycoprotein can form pseudotypes with human immunodeficiency virus type 1 virions and confers tropism for CD4-negative cells. Thus, the HERV-W env gene represents the first HERV env gene demonstrated to encode the functional properties of a retrovirus envelope glycoprotein.

scholarly journals Characterization of Human Endogenous Retrovirus Type K Virus-like Particles Generated from Recombinant Baculoviruses

Virology ◽  
1997 ◽  
Vol 233 (2) ◽  
pp. 280-291 ◽  
Author(s):  
Ralf R. Tönjes ◽  
Klaus Boller ◽  
Christiane Limbach ◽  
Raimond Lugert ◽  
Reinhard Kurth
Keyword(s):  
Endogenous Retrovirus ◽  
Recombinant Baculoviruses ◽  
Human Endogenous Retrovirus ◽  
Virus Like Particles ◽  
Type K

scholarly journals An Envelope Glycoprotein of the Human Endogenous Retrovirus HERV-W Is Expressed in the Human Placenta and Fuses Cells Expressing the Type D Mammalian Retrovirus Receptor

2000 ◽  
Vol 74 (7) ◽  
pp. 3321-3329 ◽  
Author(s):  
Jean-Luc Blond ◽  
Dimitri Lavillette ◽  
Valérie Cheynet ◽  
Olivier Bouton ◽  
Guy Oriol ◽  
...  
Keyword(s):  
Envelope Glycoprotein ◽  
Expression Vector ◽  
Physiological Role ◽  
Endogenous Retrovirus ◽  
Open Reading Frame ◽  
Human Endogenous Retrovirus ◽  
Membrane Glycoprotein ◽  
Reading Frame ◽  
Herv Family ◽  
Type D

ABSTRACT A new human endogenous retrovirus (HERV) family, termed HERV-W, was recently described (J.-L. Blond, F. Besème, L. Duret, O. Bouton, F. Bedin, H. Perron, B. Mandrand, and F. Mallet, J. Virol. 73:1175–1185, 1999). HERV-W mRNAs were found to be specifically expressed in placenta cells, and an env cDNA containing a complete open reading frame was recovered. In cell-cell fusion assays, we demonstrate here that the product of the HERV-W env gene is a highly fusogenic membrane glycoprotein. Transfection of an HERV-W Env expression vector in a panel of cell lines derived from different species resulted in formation of syncytia in primate and pig cells upon interaction with the type D mammalian retrovirus receptor. Moreover, envelope glycoproteins encoded by HERV-W were specifically detected in placenta cells, suggesting that they may play a physiological role during pregnancy and placenta formation.

scholarly journals A computational framework to assess genome-wide distribution of polymorphic human endogenous retrovirus-K in human populations

2018 ◽  
Author(s):  
Weiling Li ◽  
Lin Lin ◽  
Raunaq Malhotra ◽  
Lei Yang ◽  
Raj Acharya ◽  
...  
Keyword(s):  
Disease Risk ◽  
Sequence Data ◽  
Endogenous Retrovirus ◽  
Genomic Diversity ◽  
Human Endogenous Retrovirus ◽  
Human Populations ◽  
Whole Genome ◽  
Short Read ◽  
Reference Set ◽  
Type K

AbstractHuman Endogenous Retrovirus type K (HERV-K) is the only HERV known to be insertionally polymorphic. It is possible that HERV-Ks contribute to human disease because people differ in both number and genomic location of these retroviruses. Indeed viral transcripts, proteins, and antibody against HERV-K are detected in cancers, auto-immune, and neurodegenerative diseases. However, attempts to link a polymorphic HERV-K with any disease have been frustrated in part because population frequency of HERV-K provirus at each site is lacking and it is challenging to identify closely related elements such as HERV-K from short read sequence data. We present an integrated and computationally robust approach that uses whole genome short read data to determine the occupation status at all sites reported to contain a HERV-K provirus. Our method estimates the proportion of fixed length genomic sequence (k-mers) from whole genome sequence data matching a reference set ofk-mersunique to each HERV-K loci and applies mixture model-based clustering to account for low depth sequence data. Our analysis of 1000 Genomes Project Data (KGP) reveals numerous differences among the five KGP super-populations in the frequency of individual and co-occurring HERV-K proviruses; we provide a visualization tool to easily depict the prevalence of any combination of HERV-K among KGP populations. Further, the genome burden of polymorphic HERV-K is variable in humans, with East Asian (EAS) individuals having the fewest integration sites. Our study identifies population-specific sequence variation for several HERV-K proviruses. We expect these resources will advance research on HERV-K contributions to human diseases.Author summaryHuman Endogenous Retrovirus type K (HERV-K) is the youngest of retrovirus families in the human genome and is the only group that is polymorphic; a HERV-K can be present in one individual but absent from others. HERV-Ks could contribute to disease risk but establishing a link of a polymorphic HERV-K to a specific disease has been difficult. We develop an easy to use method that reveals the considerable variation existing among global populations in the frequency of individual and co-occurring polymorphic HERV-K, and in the total number of HERV-K that any individual has in their genome. Our study provides a global reference set of HERV-K genomic diversity and tools needed to determine the genomic landscape of HERV-K in any patient population.

scholarly journals Genomic flexibility of human endogenous retrovirus type K

Retrovirology ◽  
2013 ◽  
Vol 10 (S1) ◽  
Author(s):  
Derek Dube ◽  
Rafael Contreras-Galindo ◽  
Shirley He ◽  
Steven King ◽  
Marta Gonzalez-Hernandez ◽  
...  
Keyword(s):  
Endogenous Retrovirus ◽  
Human Endogenous Retrovirus ◽  
Type K
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