Novel Baculovirus Expression Vectors That Provide Sialylation of Recombinant Glycoproteins in Lepidopteran Insect Cells
2001 ◽
Vol 75
(13)
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pp. 6223-6227
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ABSTRACT This report describes novel baculovirus vectors designed to express mammalian β1,4-galactosyltransferase and α2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with these viral vectors, unlike cells infected with a wild-type baculovirus, produced a sialylated viral glycoprotein during the late phase of infection. Thus, the two mammalian glycosyltransferases encoded by these viral vectors are necessary and sufficient for sialylation of a foreign glycoprotein in insect cells under the conditions used in this study. While some of the new baculovirus vectors described in this study produced less, one produced wild-type levels of infectious budded virus progeny.
2005 ◽
Vol 86
(6)
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pp. 1619-1627
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1990 ◽
Vol 71
(8)
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pp. 1703-1711
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1996 ◽
Vol 54
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pp. 796-797
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2006 ◽
Vol 22
(4)
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pp. 572-580
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2006 ◽
Vol 80
(1)
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pp. 360-371
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2021 ◽
1989 ◽
Vol 9
(10)
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pp. 4364-4371
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