A mitogen-responsive promoter region that is synergistically activated through multiple signalling pathways.

A regulatory region of the human transferrin receptor gene promoter was found to be required for increased expression in response to serum or growth factors. This region contains two elements that appear to cooperate for full responsiveness. We found that sodium orthovanadate treatment of cells significantly activated expression of promoter constructs containing these elements. 12-O-Tetradecanoylphorbol-13-acetate alone induced a twofold increase in expression but acted synergistically with vanadate to generate a highly elevated level of expression. Dibutyryl cyclic AMP alone had no effect on expression, but when added together with vanadate and 12-O-tetradecanoylphorbol-13-acetate, led to superinduction of the promoter construct. Induction of expression by these reagents was delayed several hours, and the kinetics were identical to those observed for serum induction.

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A mitogen-responsive promoter region that is synergistically activated through multiple signalling pathways

Molecular and Cellular Biology ◽

10.1128/mcb.13.3.1796-1804.1993 ◽

1993 ◽

Vol 13 (3) ◽

pp. 1796-1804

Author(s):

Q Ouyang ◽

M Bommakanti ◽

W K Miskimins

Keyword(s):

Promoter Region ◽

Elevated Level ◽

Receptor Gene ◽

Regulatory Region ◽

Gene Promoter ◽

Signalling Pathways ◽

Sodium Orthovanadate ◽

Dibutyryl Cyclic Amp ◽

Human Transferrin Receptor ◽

Promoter Construct

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The Promoter Region of the Human Transferrin Receptor Gene

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1988.tb55492.x ◽

1988 ◽

Vol 526 (1 Hemochromatos) ◽

pp. 54-64 ◽

Cited By ~ 8

Author(s):

John L. Casey ◽

Bruno Jeso ◽

Krishnamurthy Rao ◽

Tracey A. Rouault ◽

Richard D. Klausner ◽

...

Keyword(s):

Transferrin Receptor ◽

Promoter Region ◽

Receptor Gene ◽

Human Transferrin ◽

Human Transferrin Receptor

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DNA methylation in the androgen receptor gene promoter region in rat prostate cancers

The Prostate ◽

10.1002/pros.10099 ◽

2002 ◽

Vol 52 (1) ◽

pp. 82-88 ◽

Cited By ~ 15

Author(s):

Satoru Takahashi ◽

Shingo Inaguma ◽

Michihisa Sakakibara ◽

Young-Man Cho ◽

Shugo Suzuki ◽

...

Keyword(s):

Dna Methylation ◽

Androgen Receptor ◽

Promoter Region ◽

Receptor Gene ◽

Gene Promoter ◽

Androgen Receptor Gene ◽

Rat Prostate ◽

Prostate Cancers ◽

Gene Promoter Region

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Functional analysis of haplotypes and promoter activity at the 5' region of the DRD2 gene and associations with schizophrenia.

10.21203/rs.3.rs-15812/v1 ◽

2020 ◽

Author(s):

Xicen Zhang ◽

Mei Ding ◽

Yi Liu ◽

Yongping Liu ◽

Jiaxin Xing ◽

...

Keyword(s):

Gene Expression ◽

Cell Lines ◽

Promoter Region ◽

Regulatory Region ◽

Gene Promoter ◽

Luciferase Reporter ◽

Drd2 Gene ◽

Functional Regions

Abstract Background: In previous studies, we researched the association of the DRD2 gene promoter region SNP loci rs7116768, rs1047479195, rs1799732, rs1799978 and schizophrenia using Sanger sequencing. rs7116768 and rs1799978 were found to be slightly associated with schizophrenia. This study investigated the effects of haplotypes consisted of the four SNPs on protein expression level in vitro and identified the functional sequence in the 5’ regulatory region of DRD2 gene which has a potential link with schizophrenia.Methods: Recombinant plasmids with haplotypes, SNPs and 13 recombinant vectors containing deletion fragments from the DRD2 gene 5' regulatory region were transfected into HEK293 and SK-N-SH cell lines. Relative luciferase activity of the haplotypes, SNPs and different sequences was compared using a dual luciferase reporter assay system.Results: Haplotype H4(G-C-InsC-G) could significantly increase the gene expression in SK-N-SH cell lines. Allele C of rs7116768, allele A of rs1047479195 and allele del C of rs1799732 could up-regulate the gene expression. There were 5~7 functional regions in the promoter region of DRD2 gene that could affect the level of gene expression.Conclusion: We cannot rule out the possibility that different haplotypes may influence DRD2 gene expression in vivo. We observed that allele C of rs7116768, allele A of rs1047479195 and allele del C of rs1799732 could up-regulate gene expression. The truncation results confirmed the existence of functional regions in the promoter region of DRD2 gene that could affect the level of gene expression.

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