scholarly journals Essential Requirement of CCAAT/Enhancer Binding Proteins in Embryogenesis

2004 ◽  
Vol 24 (22) ◽  
pp. 9744-9751 ◽  
Author(s):  
Valérie Bégay ◽  
Jeske Smink ◽  
Achim Leutz
Keyword(s):  
Transcription Factors ◽  
Binding Proteins ◽  
Single Copy ◽  
Gene Replacement ◽  
Essential Requirement ◽  
Enhancer Binding Proteins ◽  
Complete Development ◽  
Chorionic Plate ◽  
Partial Redundancy

ABSTRACT The CCAAT/enhancer binding proteins C/EBPα and C/EBPβ are related transcription factors that are important for the function of various organs in the postnatal mouse. Gene replacement and tissue culture experiments have suggested partial redundancy of both transcription factors. Here we show that mouse embryos deficient of both C/EBPα and C/EBPβ (C/EBPαβ−/−) die between embryonic day 10 (E10) and E11 and display defective placentas. In situ hybridization revealed that C/EBPα and C/EBPβ are coexpressed in the chorionic plate at E9.5 and later in the trophoblasts of the labyrinthine layer. In C/EBPαβ−/− placentas, allantoic blood vessels invaded the chorion; however, vessel expansion and development of the labyrinthine layer was impaired. Furthermore, a single copy of either C/EBPα in the absence of C/EBPβ or C/EBPβ in the absence of C/EBPα is sufficient to complete development, suggesting complementation of these C/EBPs during embryogenesis. A single copy of C/EBPα in the absence of C/EBPβ, however, fails to rescue survival after birth, suggesting haploinsufficiency of C/EBPα in newborns. Our data thus reveal novel essential, redundant, and dosage dependent functions of C/EBPs.

scholarly journals CCAAT/enhancer-binding proteins: structure, function and regulation

2002 ◽  
Vol 365 (3) ◽  
pp. 561-575 ◽  
Author(s):  
Dipak P. RAMJI ◽  
Pelagia FOKA
Keyword(s):  
Transcription Factors ◽  
Protein Interactions ◽  
Binding Proteins ◽  
Leucine Zipper ◽  
Cellular Proliferation ◽  
Basic Leucine Zipper ◽  
Enhancer Binding Proteins ◽  
C Terminus ◽  
The Family ◽  
Species Specific

CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. At least six members of the family have been isolated and characterized to date (C/EBPα—C/EBPζ), with further diversity produced by the generation of different sized polypeptides, predominantly by differential use of translation initiation sites, and extensive protein—protein interactions both within the family and with other transcription factors. The function of the C/EBPs has recently been investigated by a number of approaches, including studies on mice that lack specific members, and has identified pivotal roles of the family in the control of cellular proliferation and differentiation, metabolism, inflammation and numerous other responses, particularly in hepatocytes, adipocytes and haematopoietic cells. The expression of the C/EBPs is regulated at multiple levels during several physiological and pathophysiological conditions through the action of a range of factors, including hormones, mitogens, cytokines, nutrients and certain toxins. The mechanisms through which the C/EBP members are regulated during such conditions have also been the focus of several recent studies and have revealed an immense complexity with the potential existence of cell/tissue- and species-specific differences. This review deals with the structure, biological function and the regulation of the C/EBP family.

scholarly journals Identification of CCAAT/Enhancer-binding Proteins as Exchange Protein Activated by cAMP-activated Transcription Factors That Mediate the Induction of the SOCS-3 Gene

2008 ◽  
Vol 283 (11) ◽  
pp. 6843-6853 ◽  
Author(s):  
Stephen J. Yarwood ◽  
Gillian Borland ◽  
William A. Sands ◽  
Timothy M. Palmer
Keyword(s):  
Transcription Factors ◽  
Binding Proteins ◽  
Enhancer Binding Proteins ◽  
Exchange Protein

scholarly journals Regulation of the interleukin-2 CD28-responsive element by NF-ATp and various NF-kappaB/Rel transcription factors.

1997 ◽  
Vol 17 (5) ◽  
pp. 2605-2614 ◽  
Author(s):  
S B Maggirwar ◽  
E W Harhaj ◽  
S C Sun
Keyword(s):  
T Cells ◽  
Transcription Factors ◽  
Gene Transcription ◽  
Binding Proteins ◽  
Interleukin 2 ◽  
Activated T Cells ◽  
Costimulatory Signal ◽  
Enhancer Binding Proteins ◽  
Nf Kappab ◽  
Responsive Element

The CD28 costimulatory signal enhances antigen-mediated induction of interleukin-2 (IL-2) gene transcription through activation of an enhancer termed the CD28-responsive element (CD28RE). Although various nuclear proteins have been shown to bind to CD28RE, their in vivo functions in the regulation of this enhancer remain elusive. In this report, we show that CD28RE binds distinct transcription factors in cells treated with different mitogenic stimuli. Stimulation of the T-cell receptor (TCR) complex in the absence of a CD28 costimulatory signal induces a member of the nuclear factor of the activated T cells, NF-ATp; however, this treatment fails to activate the CD28RE enhancer activity. Significant activation of CD28RE was detected when the cells were treated with both the TCR stimulators and an anti-CD28 monoclonal antibody (anti-CD28), which induces the NF-kappaB/Rel enhancer binding proteins in addition to NF-ATp. The costimulatory activity of anti-CD28 can be further enhanced by a phorbol ester. Kinetic analyses demonstrate that activation of endogenous IL-2 gene transcription is correlated with the binding of CD28RE by NF-ATp and different NF-kappaB/Rel species. Transient-transfection studies reveal that expression of either NF-ATp or the p50-RelA NF-kappaB heterodimer leads to the potent transactivation of both the CD28RE enhancer and the intact IL-2 promoter in mitogen-stimulated cells. Remarkably, coexpression of these two families of enhancer-binding proteins in Jurkat T cells results in the transactivation of the CD28RE enhancer even in the absence of any cellular stimuli. Together, these results suggest that activation of IL-2 gene transcription by the TCR- and CD28-mediated signals involves the interaction of CD28RE with NF-ATp and various NF-kappaB/Rel transcription factors.

scholarly journals 1P123 DMA binding analyses of bHLH transcription factors by FRET measurements(Nucleic acid-binding proteins,Poster Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S54
Author(s):  
Koji HASEGAWA ◽  
Tatsushi GOTO ◽  
Daisuke KITANO ◽  
Mari KOTOURA ◽  
Fumio TOKUNAGA ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Nucleic Acid ◽  
Binding Proteins ◽  
Nucleic Acid Binding ◽  
Nucleic Acid Binding Proteins ◽  
Poster Presentations ◽  
Bhlh Transcription Factors

scholarly journals Age-dependent response of CCAAT/enhancer binding proteins following traumatic brain injury in mice

2010 ◽  
Vol 56 (1) ◽  
pp. 188-193 ◽  
Author(s):  
Rajat Sandhir ◽  
Nancy E.J. Berman
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Binding Proteins ◽  
Enhancer Binding Proteins ◽  
Age Dependent

Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences

2011 ◽  
Vol 30 (9) ◽  
pp. 1779-1786 ◽  
Author(s):  
Kun Yang ◽  
Hecui Zhang ◽  
Richard Converse ◽  
Yong Wang ◽  
Xiaoying Rong ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Single Copy ◽  
Repetitive Dna Sequences

scholarly journals A functional assay for enhancer-binding proteins

1988 ◽  
Vol 16 (20) ◽  
pp. 9870-9870 ◽  
Author(s):  
M. Hallupp ◽  
W.H. Strãtling
Keyword(s):  
Binding Proteins ◽  
Functional Assay ◽  
Enhancer Binding Proteins
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