A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo
Keyword(s):
Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.
1989 ◽
Vol 9
(1)
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pp. 329-331
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1996 ◽
Vol 134
(3)
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pp. 715-729
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1986 ◽
Vol 6
(12)
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pp. 4419-4424
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1986 ◽
Vol 6
(12)
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pp. 4419-4424
1989 ◽
Vol 86
(8)
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pp. 2602-2606
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1990 ◽
Vol 265
(4)
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pp. 2352-2357