scholarly journals Genome Sequence of the Alphaproteobacterium Blastochloris sulfoviridis DSM 729, Which Requires Reduced Sulfur as a Growth Supplement and Contains Bacteriochlorophyll b

2020 ◽  
Vol 9 (18) ◽  
Author(s):  
John A. Kyndt ◽  
Dayana Montano Salama ◽  
Terry E. Meyer

The genome sequence of Blastochloris sulfoviridis is 3.85 Mb with a GC content of 68%. Its nearest relative is B. tepida (average nucleotide identity [ANI], 91.5%), followed by B. viridis (ANI, 83%). According to ANI and whole-genome-based phylogenetic analysis, the nearest relatives of Blastochloris are Rhodoplanes and Rhodopseudomonas, confirming the recognition of distinct genera.

2020 ◽  
Vol 9 (25) ◽  
Author(s):  
Wesley D. Rhinehart ◽  
Amanda J. Laidlaw ◽  
Alexis M. O’Neal ◽  
Jessica A. Toller ◽  
Miriam Segura-Totten ◽  
...  

ABSTRACT Novel mycobacteriophage XianYue was isolated in Northeast Georgia and infects Mycobacteria smegmatis mc2155. Actinobacteriophages which share at least 50% nucleotide identity are grouped into clusters, with XianYue in cluster A2. Its genome is 52,907 bp with 91 open reading frames (ORFs) and 62.9% GC content, and it shares 86.51% nucleotide identity with mycobacteriophage Trixie.


2020 ◽  
Vol 9 (7) ◽  
Author(s):  
Tasmina Akter ◽  
M. Mahbubur Rahman ◽  
Alfred Chin Yen Tay ◽  
Rakib Ehsan ◽  
M. Tofazzal Islam

A fish-pathogenic bacterium, Enterococcus faecalis strain BFFF11, was isolated from a tilapia suffering from streptococcosis in a fish farm in the Gazipur district of Bangladesh. The whole genome of this strain, BFFF11, was 3,067,042 bp, with a GC content of 37.4%.


2020 ◽  
Vol 9 (14) ◽  
Author(s):  
Fabiola A. Aviles ◽  
Terry E. Meyer ◽  
John A. Kyndt

We have determined the draft genome sequences of Thiorhodococcus mannitoliphagus and Thiorhodococcus minor for comparison with those of T. drewsii and Imhoffiella purpurea. According to average nucleotide identity (ANI) and whole-genome phylogenetic comparisons, these two species are clearly distinct from the Imhoffiella species and T. drewsii.


2021 ◽  
Vol 10 (19) ◽  
Author(s):  
Nao Nakamichi ◽  
Ryota Moriuchi ◽  
Hideo Dohra ◽  
Hiroyuki Futamata ◽  
Yosuke Tashiro

ABSTRACT We report here the complete genome sequence of Buttiauxella agrestis DSM 9389, which harbors eight 16S rRNA genes classified into three types. The genome sequence of this strain showed a high average nucleotide identity (97.3%) with that of the highly membrane vesicle-producing strain B. agrestis ATCC 33320T.


2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Jongbum Jeon ◽  
Jung A Kim ◽  
Sook-Young Park ◽  
Gye-Won Kim ◽  
Cheon-Seok Park ◽  
...  

The fungus Aspergillus oryzae strain BP2-1 was isolated from the traditional malted starter culture nuruk. We report here the draft whole-genome sequence of A. oryzae BP2-1, which is comprised of 14 scaffolds with a total length of 39,455,382 bp and a GC content of 47.13%.


2019 ◽  
Vol 8 (29) ◽  
Author(s):  
Jamal Saad ◽  
Marine Combe ◽  
Nassim Hammoudi ◽  
Pierre Couppié ◽  
Romain Blaizot ◽  
...  

Combined Nanopore and Illumina whole-genome sequencing of a French Guianan Mycobacterium ulcerans (Buruli ulcer agent) clinical isolate yielded a 5.12-Mbp genome with a 65.5% GC content, 5,215 protein-coding genes, and 51 predicted RNA genes. This publicly available M. ulcerans whole-genome sequence from a strain isolated in South America is closely related to M. ulcerans subsp. liflandii.


2020 ◽  
Vol 9 (12) ◽  
Author(s):  
Kentaro Miyazaki ◽  
Erina Hase ◽  
Tomomasa Maruya

We isolated the soil bacterium strain PL1 and herein report its complete genome sequence. The strain presented 97% average nucleotide identity (ANI) to Bacillus cereus and 91% ANIs to other members of the B. cereus group, indicating that it is affiliated with B. cereus.


2021 ◽  
Vol 10 (17) ◽  
Author(s):  
Jorge H. García-García ◽  
Carlos J. Moreno-Peña ◽  
Cesar I. Hernández-Vásquez ◽  
Luis J. Galán-Wong ◽  
Benito Pereyra-Alférez

ABSTRACT Bacillus albus is a new species, but it lies on the borderline with Bacillus thuringiensis. In this work, we report a strain previously identified as Bacillus thuringiensis IB84, which now, based on average nucleotide identity and rRNA 16S, gyrB, groEL, and xre gene sequences, must be identified as Bacillus albus.


2020 ◽  
Author(s):  
Antonio Roberto Gomes de Farias ◽  
Wilson José da Silva Junior ◽  
José Bandeira do Nascimento Junior ◽  
Valdir de Queiroz Balbino ◽  
Ana Maria Benko-Iseppon ◽  
...  

Abstract Background Xanthomonas citri pv. viticola is one of the most critical grapevine diseases in the Northeast of Brazil, presenting a high risk to Brazilian and worldwide areas of grape production. The X.citri pv. viticola epithet was recently proposed to be changed from X. campestris pv. v iticola based on multilocus sequence analysis and whole-genome sequences. Besides, genomics has revolutionized the field of bacteriology, by associating genome sequencing with comparative analysis such as in silico analysis such as DNA-DNA hybridization, average nucleotide identity, distance between genomes, pan-genomic approach, and phylogenomic, providing valuable insights and knowledge about virulence factors and contributing to increase the understanding and clarifying the taxonomic relationship of Xanthomonas and others prokaryotic species.Results We used the whole-genome sequence of three Brazilian strains and the pathotype to characterize X.citri pv. viticola accessions plus 124 whole-genome sequences of Xanthomonas species available in NCBI, comprising 13 species and 15 pathovars. The whole-genome sequence structure of X. citri pv. viticola was shown presents a high level of conservation concerning other X. citri species. Pan-genomic approaches, average nucleotide identity analysis, and in silico DNA-DNA hybridization were carried out, allowing X.citri pv. viticola characterization and inferences on the phylogenetic relationships within Xanthomonas . The analysis of the sequence of the 128 genomes clustered the Xanthomonas strains in eight main groups according to the recently proposed classification in all approaches used. Also, the analysis revealed that X. hortorum and X. gardneri should be classified as a single species, and the strain 17 of X. campestris and XC01 of X. citri pv. mangiferaeindicae widely described in the literature are misclassified.Conclusions We performed the genomic characterization of three representative Brazilian strains of Xcv . The genomic approaches based in the pan-genome, average nucleotide identity, and in silico DNA-DNA hybridization support the proposed taxonomic position of X.citri pv. viticola and of the recently proposed Xanthomonas species and pathovars. In addition, we detected species delimitation of the misclassified Xanthomonas strains with extensive studies reported in the literature.


2020 ◽  
Vol 9 (40) ◽  
Author(s):  
Grant A. Chambers ◽  
Nerida J. Donovan ◽  
Daniel R. Bogema ◽  
Namgay Om ◽  
George A. C. Beattie ◽  
...  

ABSTRACT The draft genome sequence of a novel “Candidatus Liberibacter” species detected in an unidentified species of Zanthoxylum (Rutaceae) collected in Bhutan is reported. The total length is 1,408,989 bp with 1,169 coding sequences in 96 contigs, a GC content of 37.3%, and 76 to 77% average nucleotide identity with several other “Ca. Liberibacter” species.


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