Unspecific Effect of N-phenyl-2-naphthylamine on the Activity of the Adenylate Cyclase Signal System of the Bacterial Agent of Clavibacter michiganensis ssp. sepedonicus Potato Ring Rot

2018 ◽  
Vol 54 (3) ◽  
pp. 331-336 ◽  
Author(s):  
L. A. Lomovatskaya ◽  
A. M. Goncharova ◽  
L. E. Makarova ◽  
N. V. Filinova ◽  
A. S. Romanenko
Keyword(s):  
Adenylate Cyclase ◽  
Signal System ◽  
Clavibacter Michiganensis ◽  
Bacterial Agent ◽  
Ring Rot ◽  
Potato Ring Rot

Lateral flow immunoassay for rapid detection of potato ring rot caused by Clavibacter michiganensis subsp. sepedonicus

2014 ◽  
Vol 50 (6) ◽  
pp. 675-682 ◽  
Author(s):  
I. V. Safenkova ◽  
I. A. Zaitsev ◽  
G. K. Pankratova ◽  
Yu. A. Varitsev ◽  
A. V. Zherdev ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Clavibacter Michiganensis ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Potato Ring Rot

Evaluation of the API 50CH and API ZYM systems for rapid characterization of Clavibacter michiganensis subsp. sepedonicus, causal agent of potato ring rot

2006 ◽  
Vol 115 (4) ◽  
pp. 443-451 ◽  
Author(s):  
José Luis Palomo ◽  
María M. López ◽  
Pablo García-Benavides ◽  
Encarna Velázquez ◽  
Eustoquio Martínez-Molina
Keyword(s):  
Causal Agent ◽  
Clavibacter Michiganensis ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Potato Ring Rot ◽  
Rapid Characterization

scholarly journals Detection of Potato ring rot Pathogen Clavibacter michiganensis subsp. sepedonicus by Loop-mediated isothermal amplification (LAMP) assay

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hasan Sagcan ◽  
Neslihan Turgut Kara
Keyword(s):  
Rapid Diagnosis ◽  
Isothermal Amplification ◽  
Diagnostic Methods ◽  
Economic Losses ◽  
Temperature Cycle ◽  
Lamp Method ◽  
Clavibacter Michiganensis ◽  
Loop Mediated Isothermal Amplification ◽  
Ring Rot ◽  
Potato Ring Rot

AbstractClavibacter michiganensis subsp. sepedonicus (CMS) is an important bacterial plant pathogen causing potato ring rot disease. Rapid diagnosis of CMS is crucial because of the economic losses caused by serious harvest losses. Although there are serological tests used in the rapid diagnosis of CMS, they are not widely used because of their low sensitivity. The DNA-based PCR methods, which are highly sensitive, do not have the possibility of on-site diagnosis, especially since they require serious laboratory infrastructure. In recent years, scientists have been working on alternative amplification methods to develop DNA-based point of care (POC) diagnostic methods. Accordingly, the loop-mediated isothermal amplification (LAMP) method, which was developed in the early 2000s, provides an important convenience for DNA-based tests to use in the field. Due to the unique design of primers, more amplification products could be create in a shorter time than conventional amplification methods without needing a temperature cycle, and it can be applied with the aid of a simple heater without requiring a laboratory environment. In this study, efficient LAMP method for the detection of CMS has optimized. For device-independent detection of LAMP products, colorimetric method and LFD has used.

Clavibacter michiganensis subsp. sepedonicus (potato ring rot).

2021 ◽  
Author(s):  
Solke de Boer
Keyword(s):  
Potato Production ◽  
Potato Tubers ◽  
Clavibacter Michiganensis ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Cool Environment ◽  
Latently Infected ◽  
Important Means ◽  
Potato Ring Rot ◽  
On Farm

Abstract Clavibacter michiganensis subsp. sepedonicus has the propensity to exist asymptomatically as latent infections in potato (Solanum tuberosum); it is not known to naturally infect other plant species. Inadvertent dissemination of the bacterium to new places of production occurs with the movement of latently infected seed tubers used for planting. The bacterium also spreads from infected tubers through direct contact and by contamination of equipment used for potato production such as seed cutters, planters, harvesters, transport vehicles, storages, etc. C. michiganensis subp. sepedonicus survives for extended periods of many months to years in a dry and cool environment. Hence its persistence on farm equipment, in storages, and on transport vehicles is an important means by which the bacterium is maintained and spread within farm units and disseminated to other production units. It persists in the field in unharvested potato tubers (i.e. volunteers or ground keepers) and in infected potato plant debris.

Effect of Selenium-containing Biocomposites from Medicinal Mushrooms on the Potato Ring Rot Causative Agent

2018 ◽  
Vol 479 (1) ◽  
pp. 67-69
Author(s):  
A. I. Perfileva ◽  
O. M. Tsivileva ◽  
Ya. B. Drevko ◽  
D. N. Ibragimova ◽  
O. V. Koftin
Keyword(s):  
Causative Agent ◽  
Medicinal Mushrooms ◽  
Ring Rot ◽  
Potato Ring Rot

scholarly journals Teknik Inokulasi Buatan Clavibacter michiganensis subsp. sepedonicus, Penyebab Penyakit Busuk Cincin Bakteri, pada Tanaman Kentang (Solanum tuberosum L.)

Agrikultura ◽  
2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Luciana Djaya ◽  
Ineu Sulastrini ◽  
Iin Rusita
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Block Design ◽  
Clavibacter Michiganensis ◽  
Bacterial Ring Rot ◽  
Seed Tubers ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Inoculation Techniques ◽  
Rot Disease

ABSTRACT Inoculation Techniques of Clavibacter michiganensis subsp. sepedonicus, the Cause of Bacterial Ring Rot Disease, on Potato (Solanum tuberosum L.). Clavibacter michiganensis subsp. sepedonicus, the cause of bacterial ring rot disease on potatoes, has been detected in potato fields in Pangalengan. To anticipate the spread of the pathogen, researches on the desease epidemiology are urgent to be carried out. Artificial inoculation techniques will be useful in the epidemiological studies. The objective of this reasearch was to evaluate some inoculation techniques, which are simple, cheap and fast in causing disease symptoms. The experiment was carried out at the laboratory and glasshouse of Balai Penelitian Tanaman Sayuran (Balitsa), Lembang. The experiment was arranged in the randomized block design with five treatments of inoculation technique and five replications. The treatments were (a) soaking wounded seed tubers in pathogen suspension, (b) soaking not wounded seed tubers in pathogen suspension, (c) pathogen suspension was injected into leaf axil, (d) pathogen suspension was injected into seed tubers, and (e) pathogen suspension was poured into the planting holes. The results showed that stabbing and soaking tubers in pathogen suspension caused the shortest incubation period (17 days after inoculation) and the highest disease incidence (60%). Keywords : Clavibacter michiganensis subsp. sepedonicus, Bacterial ring rot, Potatoes, Inoculation techniquesABSTRAKClavibacter michiganensis subsp. sepedonicus (Cms), penyebab penyakit busuk cincin bakteri pada tanaman kentang, telah terdeteksi keberadaannya pada pertanaman kentang di Pangalengan. Dalam upaya mencegah penyebaran penyakit busuk cincin bakteri di Indonesia, perlu adanya studi epidemiologi patogen tersebut. Pada penelitian epidemiologi akan diperlukan cara menginokulasi tanaman secara buatan. Tujuan dari penelitian ini adalah untuk mendapatkan teknik inokulasi buatan yang dapat menyebabkan periode inkubasi yang lebih singkat dan persentase kejadian penyakit busuk cincin bakteri paling tinggi pada tanaman kentang. Percobaan dilaksanakan di rumah kaca dan laboratorium penyakit Balai Penelitian Tanaman Sayuran (Balitsa), Lembang. Percobaan dirancang secara rancangan acak kelompok dengan lima perlakuan teknik inokulasi Cms dan lima ulangan, yaitu inokulasi dengan (a) merendam ubi benih yang telah dilukai dalam suspensi bakteri, (b) merendam ubi benih tanpa dilukai dalam suspensi bakteri, (c) suspensi bakteri ditusukkan pada ketiak daun tanaman kentang, (d) suspensi bakteri ditusukkan pada ubi benih, dan (e) suspensi bakteri disiramkan pada lubang tanam. Masing-masing ulangan terdiri dari lima tanaman. Hasil penelitian menunjukkan bahwa dari lima teknik inokulasi buatan yang dilakukan, inokulasi dengan melukai ubi benih dan merendamnya dalam suspensi patogen menghasilkanJurnal Agrikultura 2016, 27 (2): 66-71ISSN 0853-2885Teknik Inokulasi Buatan Clavibacter….67periode inkubasi tersingkat, yaitu 17 hari setelah inokulasi, dan persentase kejadian penyakit tertinggi yaitu sebesar 60%.Kata Kunci : Clavibacter michiganensis subsp. sepedonicus, Busuk cincin bakteri, Kentang, Inokulasi buatan

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