Overexpression of the glutathione S-transferase gene from Pyrus pyrifolia fruit improves tolerance to abiotic stress in transgenic tobacco plants

2013 ◽  
Vol 47 (4) ◽  
pp. 515-523 ◽  
Author(s):  
D. Liu ◽  
Y. Liu ◽  
J. Rao ◽  
G. Wang ◽  
H. Li ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Transgenic Tobacco ◽  
Pyrus Pyrifolia ◽  
Transgenic Tobacco Plants ◽  
Glutathione S Transferase ◽  
Tobacco Plants ◽  
Transferase Gene

scholarly journals A Versatile Peroxidase from the Fungus Bjerkandera adusta Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 859
Author(s):  
Nancy Sofia Hernández-Bueno ◽  
Ramón Suárez-Rodríguez ◽  
Edgar Balcázar-López ◽  
Jorge Luis Folch-Mallol ◽  
José Augusto Ramírez-Trujillo ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Manganese Peroxidase ◽  
Abiotic Stress Tolerance ◽  
White Rot Fungi ◽  
White Rot ◽  
Versatile Peroxidase ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

White-rot fungi are efficient lignin degraders due to the secretion of lignin peroxidase, manganese peroxidase, laccase, and versatile peroxidase (VP) on decayed wood. The VP is a high-redox-potential enzyme and could be used to detoxify reactive oxygen species (ROS), which accumulate in plants during biotic and abiotic stresses. We cloned the VP gene and expressed it via the Agrobacterium transformation procedure in transgenic tobacco plants to assay their tolerance to different abiotic stress conditions. Thirty independent T2 transgenic VP lines overexpressing the fungal Bjerkandera adustaVP gene were selected on kanamycin. The VP22, VP24, and VP27 lines showed significant manganese peroxidase (MnP) activity. The highest was VP22, which showed 10.87-fold more manganese peroxidase activity than the wild-type plants and led to a 34% increase in plant height and 28% more biomass. The VP22, VP24, and VP27 lines showed enhanced tolerance to drought, 200 mM NaCl, and 400 mM sorbitol. Also, these transgenics displayed significant tolerance to methyl viologen, an active oxygen-generating compound. The present data indicate that overproducing the VP gene in plants increases significantly their biomass and the abiotic stress tolerance. The VP enzyme is an effective biotechnological tool to protect organisms against ROS. In transgenic tobacco plants, it improves drought, salt, and oxidative stress tolerance. Thus, the VP gene represents a great potential for obtaining stress-tolerant crops.

Transgenic tobacco plants overexpressing cotton glutathione S-transferase (GST) show enhanced resistance to methyl viologen

2003 ◽  
Vol 160 (11) ◽  
pp. 1305-1311 ◽  
Author(s):  
T.a.o. Yu ◽  
Yang Sheng Li ◽  
Xue Feng Chen ◽  
Jing Hu ◽  
X.u.n. Chang ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Methyl Viologen ◽  
Transgenic Tobacco Plants ◽  
Glutathione S Transferase ◽  
Tobacco Plants ◽  
Enhanced Resistance

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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