Experimental design of reversed-phase high performance liquid chromatographic conditions for simultaneous determination of ibuprofen, pseudoephedrine hydrochloride, chlorpheniramine maleate, and nipagen

2010 ◽  
Vol 65 (7) ◽  
pp. 743-748 ◽  
Author(s):  
B. Aşçi ◽  
Ö. A. Dönmmez ◽  
A. Bozdoğan ◽  
S. Sungur
Keyword(s):  
Experimental Design ◽  
Simultaneous Determination ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Chlorpheniramine Maleate ◽  
Liquid Chromatographic

A high-performance liquid-chromatographic method for routine simultaneous determination of nicotine and cotinine in plasma.

1988 ◽  
Vol 34 (4) ◽  
pp. 724-729 ◽  
Author(s):  
M Hariharan ◽  
T VanNoord ◽  
J F Greden
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Methylene Chloride ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Standard Curve ◽  
Liquid Chromatographic

Abstract We describe a rapid, sensitive method for the routine simultaneous determination of nicotine and cotinine in 1 mL of plasma. Extraction in 10-mL screw-capped Teflon tubes with methylene chloride after deproteinization with trichloroacetic acid eliminated emulsion formation. The extract, after evaporation and reconstitution in 30 microL of mobile phase, is injected into a reversed-phase C-18 ion-pair column of an isocratic high-performance liquid-chromatographic unit. Absorbance is monitored at 256 nm. The mobile phase is a citrate-phosphate (30 mmol each per liter) buffer mixture containing 50 mL of acetonitrile and 1 mmol of sodium heptanesulfonate per liter. 2-Phenylimidazole is the internal standard. The detection limit is 1 microgram/L for nicotine and 3 micrograms/L for cotinine. The standard curve is linear from 0 to 700 micrograms/L for both compounds. The average CV for nicotine in the concentration range 0-100 micrograms/L is 6.5%, and that for cotinine in the concentration range 50-700 micrograms/L is 4%.

scholarly journals Simultaneous Determination of Phenylephrine Hydrochloride, Guaifenesin, and Chlorpheniramine Maleate in Cough Syrup by Gradient Liquid Chromatography

2008 ◽  
Vol 91 (2) ◽  
pp. 276-284 ◽  
Author(s):  
Sawsan M Amer ◽  
Samah S Abbas ◽  
Mostafa A Shehata ◽  
Nahed M Ali
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Pure Form ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Cough Syrup ◽  
Liquid Chromatographic

Abstract A simple and reliable high-performance liquid chromatographic method was developed for the simultaneous determination of mixture of phenylephrine hydrochloride (PHENYL), guaifenesin (GUAIF), and chlorpheniramine maleate (CHLO) either in pure form or in the presence of methylparaben and propylparaben in a commercial cough syrup dosage form. Separation was achieved on a C8 column using 0.005 M heptane sulfonic acid sodium salt (pH 3.4 0.1) and acetonitrile as a mobile phase by gradient elution at different flow rates, and detection was done spectrophotometrically at 210 nm. A linear relationship in the range of 30180, 1201800, and 1060 g/mL was obtained for PHENYL, GUAIF, and CHLO, respectively. The results were statistically analyzed and compared with those obtained by applying the British Pharmacopoeia (2002) method and showed that the proposed method is precise, accurate, and can be easily applied for the determination of the drugs under investigation in pure form and in cough syrup formulations.

scholarly journals Column Reversed-Phase High-Performance Liquid Chromatographic Method for Simultaneous Determination of Rabeprazole Sodium and Domperidone in Combined Tablet Dosage Form

2008 ◽  
Vol 91 (2) ◽  
pp. 344-348 ◽  
Author(s):  
Shweta Sadanand Sabnis ◽  
Nilesh Dnvandev Dhavale ◽  
Vijay Yeshawantrao Jadhav ◽  
Santosh Vilashchand Gandhi
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Dosage Form ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Rabeprazole Sodium ◽  
Tablet Dosage ◽  
Liquid Chromatographic

Abstract A new, simple column reversed-phase high-performance liquid chromatographic (HPLC) method for simultaneous determination of rabeprazole sodium (RAB) and domperidone (DOM) in a combined tablet dosage form has been developed and validated. Determination was performed using a Jasco HPLC system with a HiQ SiL octadecylsilane (C18) column (250 4.6 mm id), acetonitrile0.1 M ammonium acetate (50 + 50, v/v) mobile phase, and paracetamol as an internal standard. The detection was performed using a UV detector set at 280 nm. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 1.010.0 and 0.55.0 g/mL for RAB and DOM, respectively. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.

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