Determination of carbohydrates and sweeteners in foods and biologically active additives by high-performance liquid chromatography

2013 ◽  
Vol 68 (12) ◽  
pp. 1081-1084 ◽  
Author(s):  
A. M. Zakharova ◽  
I. L. Grinshtein ◽  
L. A. Kartsova
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Biologically Active

scholarly journals Detection and development of a quantitation method for undeclared compounds in antidiabetic biologically active additives and its validation by high performance liquid chromatography

Pharmacia ◽  
2022 ◽  
Vol 69 (1) ◽  
pp. 45-50
Author(s):  
Virab Gurgen Kirakosyan ◽  
Avetis Hovhannes Tsaturyan ◽  
Lilit Eduard Poghosyan ◽  
Ella Vardan Minasyan ◽  
Hayk Razmik Petrosyan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Sodium Dodecyl ◽  
Biologically Active ◽  
Ich Guidelines ◽  
System Suitability ◽  
Precision And Accuracy ◽  
Quantitation Method

An isocratic, high-performance liquid chromatography (HPLC) quantitation method was developed for the quantitative determination of metformin, glibenclamide, gliclazide, glimepiride in some antidiabetic biologically active additives. A Nucleosil C18, 5 μm, 4.6 mm × 150 mm, column with mobile phase containing buffer (10 mm Na2HPO4, 10 mm sodium dodecyl sulfate): acetonitrile = 68 : 32 (V/V), pH = 7.5 was used. The flow rate was 1.0 mL/min, and effluents were monitored at 226 nm. The retention times of gliclazide glibenclamide, glimepiride and metformin, were 2.203, 4.587, 5.667 and 10.182 min, respectively. Linearity was studied by preparing standard solutions of gliclazide, glibenclamide, glimepiride and metformin at the concentration range of 50% to 150% of working concentration from a stock solution. The method was successfully applied to the estimation of gliclazide, glibenclamide, glimepiride and metformin in some antidiabetic biologically active additives. This method was validated to confirm its system suitability, selectivity, linearity, precision and accuracy according to international conference on harmonization (ICH) guidelines.

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