distant hybridization
Recently Published Documents


TOTAL DOCUMENTS

91
(FIVE YEARS 45)

H-INDEX

9
(FIVE YEARS 3)

2021 ◽  
Author(s):  
Vitaliy Puhal'skiy

The textbook outlines the cytological foundations of heredity, the regularities of Mendelian genetics, the chromosomal theory of heredity, the basics of molecular genetics and genetic engineering, the patterns of inheritance during distant hybridization, polyplodia and mutagenesis. Special attention is paid to the heterosis and genetics of populations. A significant part of the data is presented in the form of figures, diagrams, graphs and tables that facilitate the understanding of the material presented. Meets the requirements of the federal state educational standards of higher education of the latest generation. For students of higher educational institutions studying in agronomic specialties.


2021 ◽  
Vol 12 ◽  
Author(s):  
Binwen Tan ◽  
Lei Zhao ◽  
Lingyu Li ◽  
Hao Zhang ◽  
Wei Zhu ◽  
...  

Early maturation is an important objective in wheat breeding programs that could facilitate multiple-cropping systems, decrease disaster- and disease-related losses, ensure stable wheat production, and increase economic benefits. Exploitation of novel germplasm from wild relatives of wheat is an effective means of breeding for early maturity. Psathyrostachys huashanica Keng f. ex P. C. KUO (2n=2x=14, NsNs) is a promising source of useful genes for wheat genetic improvement. In this study, we characterized a novel wheat-P. huashanica line, DT23, derived from distant hybridization between common wheat and P. huashanica. Fluorescence in situ hybridization (FISH) and sequential genomic in situ hybridization (GISH) analyses indicated that DT23 is a stable wheat-P. huashanica ditelosomic addition line. FISH painting and PCR-based landmark unique gene markers analyses further revealed that DT23 is a wheat-P. huashanica 7Ns ditelosomic addition line. Observation of spike differentiation and the growth period revealed that DT23 exhibited earlier maturation than the wheat parents. This is the first report of new earliness per se (Eps) gene(s) probably associated with a group 7 chromosome of P. huashanica. Based on specific locus-amplified fragment sequencing technology, 45 new specific molecular markers and 19 specific FISH probes were developed for the P. huashanica 7Ns chromosome. Marker validation analyses revealed that two specific markers distinguished the Ns genome chromosomes of P. huashanica and the chromosomes of other wheat-related species. These newly developed FISH probes specifically detected Ns genome chromosomes of P. huashanica in the wheat background. The DT23 line will be useful for breeding early maturing wheat. The specific markers and FISH probes developed in this study can be used to detect and trace P. huashanica chromosomes and chromosomal segments carrying elite genes in diverse materials.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Libin Zhang ◽  
Jianjie He ◽  
Hongsheng He ◽  
Jiangsheng Wu ◽  
Maoteng Li

AbstractRaphanobrassica (RrRrCrCr, 2n = 4x = 36), which is generated by distant hybridization between the maternal parent Raphanus sativus (RsRs, 2n = 2x = 18) and the paternal parent Brassica oleracea (C°C°, 2n = 2x = 18), displays intermediate silique phenotypes compared to diploid progenitors. However, the hybrid shares much more similarities in silique phenotypes with those of B. oleracea than those of R. sativus. Strikingly, the silique of Raphanobrassica is obviously split into two parts. To investigate the gene expression patterns behind these phenomena, transcriptome analysis was performed on the upper, middle, and lower sections of pods (RCsiu, RCsim, and RCsil), seeds in the upper and lower sections of siliques (RCseu and RCsel) from Raphanobrassica, whole pods (Rsi and Csi) and all seeds in the siliques (Rse and Cse) from R. sativus and B. oleracea. Transcriptome shock was observed in all five aforementioned tissues of Raphanobrassica. Genome-wide unbalanced biased expression and expression level dominance were also discovered, and both of them were toward B. oleracea in Raphanobrassica, which is consistent with the observed phenotypes. The present results reveal the global gene expression patterns of different sections of siliques of Raphanobrassica, pods, and seeds of B. oleracea and R. sativus, unraveling the tight correlation between global gene expression patterns and phenotypes of the hybrid and its parents.


2021 ◽  
Vol 22 (20) ◽  
pp. 11208
Author(s):  
Zhi Zhang ◽  
Shenghui Zhou ◽  
Weihua Liu ◽  
Liqiang Song ◽  
Jinpeng Zhang ◽  
...  

Agropyroncristatum (2n = 4x = 28, PPPP) is an important wild relative of common wheat (Triticum aestivum L., 2n = 6x = 42). A previous report showed that the wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS (0.81–1.00) exhibited high resistance to prevalent physiological races of stripe rust (CYR32 and CYR33). In this study, three disease resistance-related transcripts, which were mapped to A. cristatum 6PS (0.81–1.00) through the analysis of specific molecular markers, were acquired from among A. cristatum full-length transcripts. The BC5F2 and BC5F2:3 genetic populations of the translocation line WAT655 were analyzed by using three disease resistance-related gene markers, A. cristatum P genome-specific markers, and fluorescence in situ hybridization (FISH). The results revealed that the introgression between A. cristatum P genome and wheat genome was observed in progenies of the genetic populations of the translocation line WAT655 and the physical positions of the three genes were considerably adjacent on A. cristatum 6PS (0.81–1.00) according to the FISH results. Additionally, kompetitive allele-specific PCR (KASP) markers of the three genes were developed to detect and acquire 24 breeding lines selected from the progenies of the distant hybridization of wheat and A. cristatum, which showed resistance to physiological races of stripe rust (CYR32 and CYR33) and other desirable agronomic traits according to the field investigation. In conclusion, this study not only provides new insights into the introgression between A. cristatum P genome and wheat genome but also provides the desirable germplasms for breeding practice.


2021 ◽  
pp. 5-10
Author(s):  
O. N. Pyshnaya ◽  
E. A. Dzhos

The article presents a brief history of the establishment of the Laboratory of Plant Breeding and Seed Production of Solanaceae Crops of FSBSI FSVC. The process of development of tomato, pepper, eggplant and physalis breeding is shown from the beginning of the establishment until the present time. Practical selection of Solanaceae crops in FSBSI FSVC is directly connected with the name of Academician VASKhNIL A.V. Alpatyev, who is the founder of scientific research on thermophilic crops. On the basis of conducted scientific researches the technique of creation of cold-resistant, early-ripening cultivars of tomato was developed, including influence of certain conditions on parental forms and hybrid material from its first generations to increase adaptability to new, not characteristic conditions of selected material. Subsequently, this technique was applied to the breeding of sweet pepper, eggplant and physalis. Interspecific hybridisation was used to enrich the gene pool of Solanaceous crops. As a result of distant hybridization, the following cultivars were created: the original tomato cultivar Severyanin with large parthenocarpic fruits on 1 and 2 racemes; an anadrous mutant for use in breeding; ultra-rapid, short-stage forms for obtaining heterosis effect; the cultivar Vkusny with increased content of dry matter in fruits. On the basis of interspecific hybrid of Ph. ixocarpa x Ph. angulata, the cultivars Dessert and Lakomka, characterized by high yield and resistance to diseases, high content of sugars, pectin and absence of bitterness, are created. Types of pepper Capsicum chinense, C. frutescens and C. annuum were used to create resistant forms to viral diseases. Involvement of wild eggplant species (Solanum aethiopicum, S. macrocarpon) to the breeding process allowed to obtain hybrid combinations with flavonoids content 1,3 times higher and phenolcarboxylic acids 1,6–1,7 times higher than in S. melongena.The development of theoretical bases ofSolanaceae crops breeding for resistance to biotic and abiotic stressors, as well as for high quality production, allowed to create a series of cosmopolitan cultivars, which at present are in demand, both in production and as a source material. The current scientific priorities are to use innovative biotechnology and molecular marking methods to accelerate the breeding process and improve selection efficiency.


2021 ◽  
pp. 1-6
Author(s):  
Karminderbir Kaur ◽  
Mehak Gupta ◽  
Yogesh Vikal ◽  
Kuldeep Singh ◽  
Kumari Neelam

Abstract Distant hybridization of cereals is often impaired by fertilization barriers. Haploid induction through intergeneric crossing is well developed in wheat but has not been successful in rice due to incompatibility issues. The present study was thus undertaken to identify fertilization barriers that hinder the compatibility of the rice cultivar Punjab Rice 121 with maize and pearl millet lines as pollinators. A total of 37,357 spikelets were pollinated, yielding 494 caryopses upon supplementation with auxins. The resultant caryopses, arising from true intergeneric crosses, lacked embryos. Imaging of the pollinated pistils at different intervals indicated that intense callose depositions block the release of generative nuclei to the ovule in these wide crosses. Rice spikelets pollinated with rice pollen (cis-generic crosses) exhibited positive indicators of fertilization reaction at the micropyle. While the cis-generic crosses initiated true caryopsis formation after 24 h, no comparative reaction was observed in the intergeneric crosses. The current survey underlines that the rice female gametophyte presents a strong pre-fertilization barrier to foreign pollen. This barrier may be modulated in the future by altering genotype and auxin combinations.


2021 ◽  
pp. 27-33
Author(s):  
E. A. Dzhos ◽  
D. V. Shumilina ◽  
O. N. Pyshnaya ◽  
M. I. Mamedov ◽  
A. A. Baikov ◽  
...  

Relevance. Pepper is a common crop both for fresh consumption and for the preparation of spices. Recently, along with the increasing popularity of C. annuum L. pepper, there is increasing interest in other species of this genus, which have a number of breeding and important properties. The most important method of enriching the gene pool of cultivated plants is distant hybridization, through which valuable traits are transferred from wild species to cultivated ones. The development of a new variety is a lengthy process, stretching over several years. In this regard, breeders have faced the challenge of obtaining pure lines to create a pepper hybrid with desired properties by applying modern biotechnological methods that will accelerate this process. One of them is the method of microspore culture, which allows mass production of haploid plants, reducing the time for creating constant parental lines.Material and methods. The aim of the work was to create an interspecific hybrid of hot pepper (C. annuum L. x C. frutescens L.) with high ornamental properties, a complex of economically valuable traits, with good taste qualities. The research was carried out in the film greenhouse of FSBSI FSVC in the Moscow region. The research material was a variety population of hot pepper Capsicum frutescens Cz-544-14, used as a paternal line, which was heterogeneous, and a pure line of C. annuum L. (Pb-551) created by classical breeding.Results. The pepper hybrid F1 Christmas bouquet was created as a result of hybridization of species parental forms obtained by different methods (biotechnological and classical). To accelerate the production of an aligned paternal form of C. frutescens L., the technology of doubled haploids through microspore culture was used. As a result, doubled haploid plants meeting the planned model (compact low habit, purple fruit colouring in technical ripeness and red in biological ripeness) were obtained. The resulting hybrid combined all the necessary economic features: high ornamentality, compactness, bouquet arrangement of fruits, high taste and aroma. Thus, the use of remote interspecific hybridization in the breeding process in combination with biotechnological approaches can accelerate the production of new forms of hot peppers that meet the demands of the market.


Author(s):  
Sujuan Xu ◽  
Huizhong Hou ◽  
Ze Wu ◽  
Jingya Zhao ◽  
Fengjiao Zhang ◽  
...  

Abstract Embryo abortion often occurs during distant hybridization events. Apetala 2/ethylene-responsive factor (AP2/ERF) proteins are key transcription factor (TF) regulators of plant development and stress resistance, but their roles in hybrid embryo development are poorly understood. We isolated a novel AP2/ERF TF, CmERF12, from chrysanthemum and showed that it adversely affects embryo development during distant hybridization. Transcriptome and real-time quantitative PCR data demonstrated that CmERF12 is expressed at significantly higher levels in aborted ovaries compared with normal ovaries. CmERF12 localizes to the cell nucleus and contains a conserved EAR motif that mediates its transcription repressor function in yeast and plant cells. We generated an amiR-CmERF12 transgenic Chrysanthemum morifolium (C.m.) var. ‘Yuhualuoying’ and conducted distant hybridization with the wild-type tetraploid, Chrysanthemum nankingense (C.n.), revealing that CmERF12 knockdown significantly promoted embryo development and increased the seed setting rates during hybridization. The expression of various embryo development-related genes was up-regulated in developing ovaries from the ♀amiR-CmERF12-C.m. × ♂C.n. cross. Furthermore, CmERF12 directly interacted with CmSUF4 and significantly reduced its ability to activate its target gene CmEC1. Overall, we invented an original method to overcome plant distant hybridization barriers and unraveled the mechanism by which CmERF12 negatively affects chrysanthemum embryo development.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Sujuan Xu ◽  
Ze Wu ◽  
Huizhong Hou ◽  
Jingya Zhao ◽  
Fengjiao Zhang ◽  
...  

AbstractDistant hybridization is widely used to develop crop cultivars, whereas the hybridization process of embryo abortion often severely reduces the sought-after breeding effect. The LEAFY COTYLEDON1 (LEC1) gene has been extensively investigated as a central regulator of seed development, but it is far less studied in crop hybridization breeding. Here we investigated the function and regulation mechanism of CmLEC1 from Chrysanthemum morifolium during its seed development in chrysanthemum hybridization. CmLEC1 encodes a nucleic protein and is specifically expressed in embryos. CmLEC1’s overexpression significantly promoted the seed-setting rate of the cross, while the rate was significantly decreased in the amiR-CmLEC1 transgenic chrysanthemum. The RNA-Seq analysis of the developing hybrid embryos revealed that regulatory genes involved in seed development, namely, CmLEA (late embryogenesis abundant protein), CmOLE (oleosin), CmSSP (seed storage protein), and CmEM (embryonic protein), were upregulated in the OE (overexpressing) lines but downregulated in the amiR lines vs. wild-type lines. Future analysis demonstrated that CmLEC1 directly activated CmLEA expression and interacted with CmC3H, and this CmLEC1–CmC3H interaction could enhance the transactivation ability of CmLEC1 for the expression of CmLEA. Further, CmLEC1 was able to induce several other key genes related to embryo development. Taken together, our results show that CmLEC1 plays a positive role in the hybrid embryo development of chrysanthemum plants, which might involve activating CmLEA’s expression and interacting with CmC3H. This may be a new pathway in the LEC1 regulatory network to promote seed development, one perhaps leading to a novel strategy to not only overcome embryo abortion during crop breeding but also increase the seed yield.


Sign in / Sign up

Export Citation Format

Share Document