Morphological Study of Chondrospheres Treated with Cytochalasin D

2021 ◽  
Vol 15 (6) ◽  
pp. 518-521
Author(s):  
A. A. Gryadunova ◽  
E. A. Bulanova ◽  
E. V. Koudan ◽  
V. A. Kasyanov ◽  
Yu. D. Khesuani ◽  
...  
1995 ◽  
Vol 83 (2-3) ◽  
pp. 149-161 ◽  
Author(s):  
Sabine Loty ◽  
Nadine Forest ◽  
Habib Boulekbache ◽  
Jean-Michel Sautier

Author(s):  
Susan B.G. Debaene ◽  
John S. Gardner ◽  
Phil S. Allen

The coleorhiza is a nonvascular sheath that encloses the embryonic radicle in Poaceae, and is generally the first tissue to emerge during germination. Delicate hairlike extensions develop from some coleorhiza cells prior to radicle emergence. Similar to root hairs, coleorhiza hairs are extremely sensitive to desiccation and are damaged by exposure to negative water potentials. The coleorhiza of Lolium perenne is somewhat spherical when first visible, after which a knob forms at a right angle to the caryopsis due to inner pressure from the elongating radicle. This knob increases in length until the radicle finally punctures the coleorhiza. Standard fixation procedures cause severe desiccation of coleorhiza cells and hairs, making morphological study of the coleorhiza difficult. This study was conducted to determine a more successful process for coleorhiza preservation.


1976 ◽  
Vol 14 (2) ◽  
pp. 123 ◽  
Author(s):  
Kye Heon Jeong ◽  
Han Jong Rim ◽  
He Young Yang ◽  
Woo Kap Kim ◽  
Chang Whan Kim

1967 ◽  
Vol 75 (5) ◽  
pp. 207-223 ◽  
Author(s):  
Takuro SAKAI ◽  
Izumi SASAKI ◽  
Hajime HANAMURA
Keyword(s):  

2011 ◽  
Vol 4 (2) ◽  
pp. 34-37
Author(s):  
Dr. Qudusia sultana ◽  
◽  
Dr. M.H Shariff Dr. M.H Shariff ◽  
Dr. Meera Jacob ◽  
Dr.Chitra P Rao ◽  
...  

2001 ◽  
Vol 16 (2) ◽  
pp. 75-81 ◽  
Author(s):  
Gracinda De Lourdes Jorge ◽  
Luiz Sergio Leonardi ◽  
Ilka de Fatima Santana Ferreira Boin ◽  
Orlando de Castro e Silva Jr ◽  
Cecilia Amelia Fazzio Escanhoela

The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.


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