Nonselective voltage-gated ionic channels in type II taste cells

2009 ◽  
Vol 3 (1) ◽  
pp. 71-80
Author(s):  
R. A. Romanov ◽  
N. V. Kabanova ◽  
S. L. Malkin ◽  
S. S. Kolesnikov
Keyword(s):  
Ionic Channels ◽  
Type Ii ◽  
Voltage Gated ◽  
Taste Cells

scholarly journals How do taste cells lacking synapses mediate neurotransmission? CALHM1, a voltage-gated ATP channel

BioEssays ◽  
2013 ◽  
Vol 35 (12) ◽  
pp. 1111-1118 ◽  
Author(s):  
Akiyuki Taruno ◽  
Ichiro Matsumoto ◽  
Zhongming Ma ◽  
Philippe Marambaud ◽  
J. Kevin Foskett
Keyword(s):  
Voltage Gated ◽  
Taste Cells

scholarly journals Quantal and Nonquantal Transmission in Calyx-Bearing Fibers of the Turtle Posterior Crista

2007 ◽  
Vol 98 (3) ◽  
pp. 1083-1101 ◽  
Author(s):  
Joseph C. Holt ◽  
Shilpa Chatlani ◽  
Anna Lysakowski ◽  
Jay M. Goldberg
Keyword(s):  
Ampa Receptor ◽  
Hair Cells ◽  
Ultrastructural Study ◽  
Nerve Fibers ◽  
Type I ◽  
Outer Face ◽  
Type Ii ◽  
Intracellular Recordings ◽  
External Concentration ◽  
Voltage Gated

Intracellular recordings were made from nerve fibers in the posterior ampullary nerve near the neuroepithelium. Calyx-bearing afferents were identified by their distinctive efferent-mediated responses. Such fibers receive inputs from both type I and type II hair cells. Type II inputs are made by synapses on the outer face of the calyx ending and on the boutons of dimorphic fibers. Quantal activity, consisting of brief mEPSPs, is reduced by lowering the external concentration of Ca2+ and blocked by the AMPA-receptor antagonist CNQX. Poisson statistics govern the timing of mEPSPs, which occur at high rates (250–2,500/s) in the absence of mechanical stimulation. Excitation produced by canal-duct indentation can increase mEPSP rates to nearly 5,000/s. As the rate increases, mEPSPs can change from a monophasic depolarization to a biphasic depolarizing–hyperpolarizing sequence, both of whose components are blocked by CNQX. Blockers of voltage-gated currents affect mEPSP size, which is decreased by TTX and is increased by linopirdine. mEPSP size decreases severalfold after impalement. The size decrease, although it may be triggered by the depolarization occurring during impalement, persists even at hyperpolarized membrane potentials. Nonquantal transmission is indicated by shot-noise calculations and by the presence of voltage modulations after quantal activity is abolished pharmacologically. An ultrastructural study shows that inner-face inputs from type I hair cells outnumber outer-face inputs from type II hair cells by an almost 6:1 ratio.

scholarly journals Expression and Secretion of TNF-α in Mouse Taste Buds: A Novel Function of a Specific Subset of Type II Taste Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e43140 ◽  
Author(s):  
Pu Feng ◽  
Hang Zhao ◽  
Jinghua Chai ◽  
Liquan Huang ◽  
Hong Wang
Keyword(s):  
Taste Buds ◽  
Type Ii ◽  
Specific Subset ◽  
Tnf Α ◽  
Taste Cells ◽  
Mouse Taste

Inhibition of voltage-gated calcium currents in type II vestibular hair cells by cinnarizine

2004 ◽  
Vol 369 (6) ◽  
pp. 570-575 ◽  
Author(s):  
Sonja F. Arab ◽  
Philip D�wel ◽  
Eberhard J�ngling ◽  
Martin Westhofen ◽  
Andreas L�ckhoff
Keyword(s):  
Hair Cells ◽  
Calcium Currents ◽  
Type Ii ◽  
Voltage Gated ◽  
Vestibular Hair Cells

Voltage-gated ionic channels in cultured rabbit articular chondrocytes

1996 ◽  
Vol 115 (3) ◽  
pp. 223-232 ◽  
Author(s):  
Tadashi Sugimoto ◽  
Masami Yoshino ◽  
Masato Nagao ◽  
Sciichi Ishii ◽  
Hideyo Yabu
Keyword(s):  
Articular Chondrocytes ◽  
Ionic Channels ◽  
Voltage Gated ◽  
Rabbit Articular Chondrocytes

scholarly journals Identification of electrophysiologically distinct cell subpopulations in Necturus taste buds.

1993 ◽  
Vol 102 (1) ◽  
pp. 143-170 ◽  
Author(s):  
A Bigiani ◽  
S D Roper
Keyword(s):  
Lucifer Yellow ◽  
Taste Buds ◽  
Taste Bud ◽  
Basal Cells ◽  
Patch Clamp Technique ◽  
Lingual Epithelium ◽  
Voltage Gated ◽  
Receptor Cells ◽  
Taste Cells ◽  
K Currents

We used the patch clamp technique to record from taste cells in thin transverse slices of lingual epithelium from Necturus maculosus. In this preparation, the epithelial polarity and the cellular organization of the taste buds, as well as the interrelationships among cells within the taste bud, were preserved. Whole-cell recording, combined with cell identification using Lucifer yellow, allowed us to identify distinct subpopulations of taste cells based on their electrophysiological properties. Receptor cells could be divided in two groups: one group was characterized by the presence of voltage-gated Na+, K+, and Ca2+ currents; the other group was characterized by the presence of K+ currents only. Therefore, receptor cells in the first group would be expected to be capable of generating action potentials, whereas receptor cells in the second group would not. Basal taste cells could also be divided into two different groups. Some basal cells possessed voltage-gated Na+, K+, and Ca2+ conductances, whereas other basal cells only had K+ conductance. In addition to single taste cells, we were able to identify electrically coupled taste cells. We monitored cell-cell coupling by measuring membrane capacitance and by observing Lucifer yellow dye coupling. Electrical coupling in pairs of dye-coupled taste receptor cells was strong, as indicated by experiments with the uncoupling agent 1-octanol. Electrically coupled receptor cells possessed voltage-gated currents, including Na+ and K+ currents. The electrophysiological differentiation among taste cells presumably is related to functional diversifications, such as different chemosensitivities.

scholarly journals A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli

2019 ◽  
Author(s):  
Debarghya Dutta Banik ◽  
Eric D. Benfey ◽  
Laura E. Martin ◽  
Kristen E. Kay ◽  
Gregory C. Loney ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Taste Receptor ◽  
Live Cell ◽  
Type I ◽  
Type Ii Cells ◽  
Type Ii ◽  
Type Iii ◽  
Umami Taste ◽  
Taste Cells ◽  
Multiple Signaling

ABSTRACTTaste receptor cells use multiple signaling pathways to detect chemicals in potential food items. These cells are functionally grouped into different types: Type I cells act as support cells and have glial-like properties; Type II cells detect bitter, sweet, and umami taste stimuli; and Type III cells detect sour and salty stimuli. We have identified a new population of taste cells that are broadly tuned to multiple taste stimuli including bitter, sweet, sour and umami. The goal of this study was to characterize these broadly responsive (BR) taste cells. We used an IP3R3-KO mouse (does not release calcium (Ca2+) from Type II cells when stimulated with bitter, sweet or umami stimuli) to characterize the BR cells without any potentially confounding input from Type II cells. Using live cell Ca2+ imaging in isolated taste cells from the IP3R3-KO mouse, we found that BR cells are a subset of Type III cells that respond to sour stimuli but also use a PLCβ3 signaling pathway to respond to bitter, sweet and umami stimuli. Unlike Type II cells, individual BR cells are broadly tuned and respond to multiple stimuli across different taste modalities. Live cell imaging in a PLCβ3-KO mouse confirmed that BR cells use a PLCβ3 signaling pathway to generate Ca2+ signals to bitter, sweet and umami stimuli. Analysis of c-Fos activity in the nucleus of the solitary tract (NTS) and short term behavioral assays revealed that BR cells make significant contributions to taste.

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