scholarly journals High anti-collagen type-II antibody levels and induction of proinflammatory cytokines by anti-collagen antibody-containing immune complexes in vitro characterise a distinct rheumatoid arthritis phenotype associated with acute inflammation at the time of disease onset

2006 ◽  
Vol 66 (4) ◽  
pp. 537-541 ◽  
Author(s):  
M. Mullazehi ◽  
L. Mathsson ◽  
J. Lampa ◽  
J. Ronnelid
Keyword(s):  
Rheumatoid Arthritis ◽  
Proinflammatory Cytokines ◽  
Immune Complexes ◽  
Acute Inflammation ◽  
Collagen Type ◽  
Disease Onset ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Antibody Levels

Phenotypic maintenance of articular chondrocytes in vitro requires BMP activity

2007 ◽  
Vol 20 (03) ◽  
pp. 185-191 ◽  
Author(s):  
A. O. Oshin ◽  
E. Caporali ◽  
C. R. Byron ◽  
A. A. Stewart ◽  
M. C. Stewart
Keyword(s):  
Articular Cartilage ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Soluble Form ◽  
Mrna Levels ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Endogenous Expression ◽  
Chondrocytic Phenotype

SummaryArticular chondrocytes are phenotypically unique cells that are responsible for the maintenance of articular cartilage. The articular chondrocytic phenotype is influenced by a range of soluble factors. In particular, members of the bone morphogenetic protein (BMP) family support the articular chondrocytic phenotype and stimulate synthesis of cartilaginous matrix. This study was carried out to determine the importance of BMPs in supporting the differentiated phenotype of articular chondrocytes in vitro. Exogenous BMP-2 supported expression of collagen type II and aggrecan in monolayer chondrocyte cultures, slowing the dedifferentiation process that occurs under these conditions. In contrast, BMP-2 had little effect on expression of these genes in three-dimensional aggregate cultures. Endogenous BMP-2 expression was lost in monolayer cultures, coincident with the down-regulation of collagen type II and aggrecan mRNAs, whereas BMP-2 mRNA levels were stable in aggregate cultures. Antagonism of endogenous BMP activity in aggregate cultures by Noggin or a soluble form of the BMP receptor resulted in reduced expression of collagen type II and aggrecan mRNAs, reduced collagen type II protein and sulfated glycosaminoglycan (GAG) deposition into the aggregate matrices and reduced secretion of GAGs into the culture media. These results indicate that endogenous BMPs are required for maintenance of the differentiated articular chondrocytic phenotype in vitro. These findings are of importance to cell-based strategies designed to repair articular cartilage. Articular chondrocytes require conditions that will support endogenous expression of BMPs to maintain the specialized phenotype of these cells.

scholarly journals Cartilage-specific autoimmunity in rheumatoid arthritis: characterization of a triple helical B cell epitope in the integrin-binding-domain of collagen type II

2001 ◽  
Vol 31 (6) ◽  
pp. 1666-1673 ◽  
Author(s):  
Hans-Georg Kraetsch ◽  
Christine Unger ◽  
Patrik Wernhoff ◽  
Christian Schneider ◽  
Joachim R. Kalden ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
B Cell ◽  
Collagen Type ◽  
Cell Epitope ◽  
Binding Domain ◽  
Type Ii ◽  
Collagen Type Ii ◽  
B Cell Epitope

scholarly journals Expression of the Heparan Sulfate Proteoglycan, Perlecan, during Mouse Embryogenesis and Perlecan Chondrogenic Activity In Vitro

1999 ◽  
Vol 145 (5) ◽  
pp. 1103-1115 ◽  
Author(s):  
M.M. French ◽  
S.E. Smith ◽  
K. Akanbi ◽  
T. Sanford ◽  
J. Hecht ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Collagen Type ◽  
Heparan Sulfate Proteoglycan ◽  
Surrounding Tissue ◽  
Collagen Type Iv ◽  
Type Ii ◽  
Sulfate Proteoglycan ◽  
Collagen Type Ii ◽  
Murine Embryonic Fibroblast

Expression of the basement membrane heparan sulfate proteoglycan (HSPG), perlecan (Pln), mRNA, and protein has been examined during murine development. Both Pln mRNA and protein are highly expressed in cartilaginous regions of developing mouse embryos, but not in areas of membranous bone formation. Initially detected at low levels in precartilaginous areas of d 12.5 embryos, Pln protein accumulates in these regions through d 15.5 at which time high levels are detected in the cartilage primordia. Laminin and collagen type IV, other basal lamina proteins commonly found colocalized with Pln, are absent from the cartilage primordia. Accumulation of Pln mRNA, detected by in situ hybridization, was increased in d 14.5 embryos. Cartilage primordia expression decreased to levels similar to that of the surrounding tissue at d 15.5. Pln accumulation in developing cartilage is preceded by that of collagen type II. To gain insight into Pln function in chondrogenesis, an assay was developed to assess the potential inductive activity of Pln using multipotential 10T1/2 murine embryonic fibroblast cells. Culture on Pln, but not on a variety of other matrices, stimulated extensive formation of dense nodules reminiscent of embryonic cartilaginous condensations. These nodules stained intensely with Alcian blue and collagen type II antibodies. mRNA encoding chondrocyte markers including collagen type II, aggrecan, and Pln was elevated in 10T1/2 cells cultured on Pln. Human chondrocytes that otherwise rapidly dedifferentiate during in vitro culture also formed nodules and expressed high levels of chondrocytic marker proteins when cultured on Pln. Collectively, these studies demonstrate that Pln is not only a marker of chondrogenesis, but also strongly potentiates chondrogenic differentiation in vitro.

scholarly journals Humoral immune response to citrullinated collagen type II determinants in early rheumatoid arthritis

2005 ◽  
Vol 35 (5) ◽  
pp. 1643-1652 ◽  
Author(s):  
Harald Burkhardt ◽  
Bettina Sehnert ◽  
Robert Bockermann ◽  
Åke Engström ◽  
Jochen R. Kalden ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Immune Response ◽  
Early Rheumatoid Arthritis ◽  
Humoral Immune Response ◽  
Collagen Type ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Humoral Immune

scholarly journals Cross-Reactivity between the Rheumatoid Arthritis-Associated Motif EQKRAA and Structurally Related Sequences Found inProteus mirabilis

1999 ◽  
Vol 67 (6) ◽  
pp. 2769-2775 ◽  
Author(s):  
Harmale Tiwana ◽  
Clyde Wilson ◽  
Alison Alvarez ◽  
Ramadan Abuknesha ◽  
Sukhvinder Bansal ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Collagen Type ◽  
Molecular Mimicry ◽  
Cross Reactivity ◽  
Mouse Fibroblast ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Negative Bacterium ◽  
Underlying Mechanisms ◽  
Similar Peptide

ABSTRACT Cross-reactivity or molecular mimicry may be one of the underlying mechanisms involved in the etiopathogenesis of rheumatoid arthritis (RA). Antiserum against the RA susceptibility sequence EQKRAA was shown to bind to a similar peptide ESRRAL present in the hemolysin of the gram-negative bacterium Proteus mirabilis, and an anti-ESRRAL serum reacted with EQKRAA. There was no reactivity with either anti-EQKRAA or anti-ESRRAL to a peptide containing the EDERAA sequence which is present in HLA-DRB1∗0402, an allele not associated with RA. Furthermore, the EQKRAA and ESRRAL antisera bound to a mouse fibroblast transfectant cell line (Dap.3) expressing HLA-DRB1∗0401 but not to DRB1∗0402. However, peptide sequences structurally related to the RA susceptibility motif LEIEKDFTTYGEE (P. mirabilisurease), VEIRAEGNRFTY (collagen type II) and DELSPETSPYVKE (collagen type XI) did not bind significantly to cell lines expressing HLA-DRB1∗0401 or HLA-DRB1∗0402 compared to the control peptide YASGASGASGAS. It is suggested here that molecular mimicry between HLA alleles associated with RA and P. mirabilis may be relevant in the etiopathogenesis of the disease.

IMMUNE COMPLEXES, BUT NOT STREPTOCOCCAL CELL WALLS OR ZYMOSAN, CAUSE CHRONIC ARTHRITIS IN MOUSE STRAINS SUSCEPTIBLE FOR COLLAGEN TYPE II AUTO-IMMUNE ARTHRITIS

Cytokine ◽  
1999 ◽  
Vol 11 (12) ◽  
pp. 1046-1056 ◽  
Author(s):  
Arjen B Blom ◽  
Peter L.E.M van Lent ◽  
Astrid E.M Holthuysen ◽  
Wim B van den Berg
Keyword(s):  
Immune Complexes ◽  
Cell Walls ◽  
Collagen Type ◽  
Chronic Arthritis ◽  
Mouse Strains ◽  
Type Ii ◽  
Collagen Type Ii
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