Pancreatitis associated protein as an early marker of acute pancreatitis.

We investigated pancreatic gene expression in the rat in response to taurocholate-induced acute pancreatitis. Concentrations of transcripts encoding pancreatic protein showed noncoordinated alterations. Contents in amylase, trypsinogen I, chymotrypsinogen B, elastase 1, and procarboxypeptidase A mRNAs decreased by greater than 50% during the acute phase (days 0-2), whereas actin and lithostathine mRNAs increased 5 and 0.6 times, respectively, and pancreatitis-associated protein (PAP) mRNA increased greater than 200 times, indicating redirection of the pattern of gene expression. Synthesis of pancreatic proteins was also altered in a noncoordinated manner. During the acute phase, it decreased more for trypsinogen I and chymotrypsinogen B than for amylase and lipase, whereas synthesis of the PAP increased dramatically. For amylase and chymotrypsinogen B, we compared the patterns of changes in mRNA concentrations, rates of synthesis, and pancreatic contents. Changes in enzyme contents and synthetic rates were temporally correlated during the acute phase. On the contrary, changes in mRNA concentrations and enzyme synthesis were not coordinated, suggesting that control of synthesis partly occurred at the posttranscriptional level. It was concluded that induction of pancreatitis is accompanied by transcriptional and posttranscriptional modifications resulting in rapid and massive rearrangement of the pattern of pancreatic protein gene expression.

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PAP, a pancreatic secretory protein induced during acute pancreatitis, is expressed in rat intestine

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1993.265.4.g611 ◽

1993 ◽

Vol 265 (4) ◽

pp. G611-G618 ◽

Cited By ~ 3

Author(s):

J. L. Iovanna ◽

V. Keim ◽

A. Bosshard ◽

B. Orelle ◽

J. M. Frigerio ◽

...

Keyword(s):

Acute Pancreatitis ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Secretory Protein ◽

Enzyme Linked Immunosorbent Assay ◽

Rat Intestine ◽

Mrna Concentration ◽

Initial Content ◽

Pancreatitis Associated Protein

The pancreatitis-associated protein (PAP) is a lectin-related secretory protein present in small amounts in the rat pancreas and rapidly overexpressed during the acute phase of pancreatitis. We demonstrate in this report that PAP is also expressed in rat intestine. A cDNA library from rat jejunum was probed with pancreatic PAP cDNA. The inserts of the selected recombinant clones corresponded to a transcript whose nucleotide sequence was identical to that of pancreatic PAP mRNA. The transcript was detected in duodenum, jejunum, ileum, and colon. A protein with same molecular mass (16 kDa) and pI (8.2) as pancreatic PAP was actually immunodetected in ileum homogenate after separation by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intestinal PAP was immunolocalized to the epithelial cells of the lower part of the villi. The protein accounted respectively for 0.02, 0.05, and 0.1% of soluble proteins in duodenum, jejunum, and ileum homogenates, as measured by enzyme-linked immunosorbent assay, and could not be detected in stomach and colon. Influence of fasting and feeding on PAP mRNA concentration was analyzed in ileum. Concentration decreased by 81 and 94% after animals were fasted for 24 and 48 h, respectively. Feeding restored the initial content within 6 h. On the other hand, intestinal PAP mRNA concentration was not altered during acute pancreatitis

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Already existing pseudocysts in acute pancreatitis can be an early marker of chronic pancreatitis - Analysis of 1270 cases from a prospective cohort

Pancreatology ◽

10.1016/j.pan.2020.07.162 ◽

2020 ◽

Vol 20 ◽

pp. S96-S97

Author(s):

N. Vörhendi ◽

B. Tinusz ◽

N. Gede ◽

Vincze î ◽

T. Takács ◽

...

Keyword(s):

Acute Pancreatitis ◽

Chronic Pancreatitis ◽

Prospective Cohort ◽

Early Marker

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p8 Improves Pancreatic Response to Acute Pancreatitis by Enhancing the Expression of the Anti-inflammatory Protein Pancreatitis-associated Protein I

Journal of Biological Chemistry ◽

10.1074/jbc.m309152200 ◽

2003 ◽

Vol 279 (8) ◽

pp. 7199-7207 ◽

Cited By ~ 83

Author(s):

Sophie Vasseur ◽

Emma Folch-Puy ◽

Verena Hlouschek ◽

Stephane Garcia ◽

Fritz Fiedler ◽

...

Keyword(s):

Acute Pancreatitis ◽

Inflammatory Protein ◽

Anti Inflammatory ◽

Pancreatitis Associated Protein

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SP214DETERMINATION OF NEUTROPHIL GELATINASE - ASSOCIATED LIPOCALIN AS EARLY MARKER OF ACUTE KIDNEY INJURY IN ACUTE PANCREATITIS

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfw162.33 ◽

2016 ◽

Vol 31 (suppl_1) ◽

pp. i157-i158

Author(s):

Agnieszka Gala-Ba˛dzińska ◽

Mateusz Sporek ◽

Paulina Dumnicka ◽

Marek Kuzniewski ◽

Malgorzata Mazur-Laskowska ◽

...

Keyword(s):

Acute Kidney Injury ◽

Acute Pancreatitis ◽

Kidney Injury ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Early Marker ◽

Neutrophil Gelatinase

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Macrophage migration inhibitory factor is an early marker of severe acute pancreatitis based on the revised Atlanta classification

BMC Gastroenterology ◽

10.1186/s12876-020-01598-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Dingcheng Shen ◽

Caixi Tang ◽

Shuai Zhu ◽

Gengwen Huang

Keyword(s):

Acute Pancreatitis ◽

Severe Acute Pancreatitis ◽

Migration Inhibitory Factor ◽

Predictive Value ◽

Macrophage Migration Inhibitory Factor ◽

Serum Markers ◽

Apache Ii ◽

Inhibitory Factor ◽

Macrophage Migration ◽

Early Marker

Abstract Background Various serum markers for early identification of severe acute pancreatitis (SAP) have been studied. Serum macrophage migration inhibitory factor (MIF) was reported to be correlated with severity of acute pancreatitis (AP) based on the 1992 Atlanta classification. However, MIF has never been proven to be predictive of disease severity based on the revised Atlanta classification (RAC). The potential predictive value of MIF needs to be further validated. Methods Consecutive patients with AP within 48 h after symptom onset and 10 healthy control volunteers were enrolled prospectively. Serum MIF levels were measured by enzyme-linked immunosorbent assay (ELISA). The predictive value of MIF, clinical scores and other serum markers were determined. Results Among 143 patients with AP, there were 52 (36.4%), 65 (45.5%) and 26 (18.1%) with mild, moderate and severe disease based on the RAC respectively. Compared with healthy volunteers, serum levels of MIF were significantly higher in AP patients, especially those with SAP (P < 0.001). Multivariate regression analysis indicated that increased serum MIF (cut-off 2.30 ng/ml, OR = 3.16, P = 0.008), IL-6 (cut-off 46.8 pg/ml, OR = 1.21, P = 0.043), APACHE II score (cut-off 7.5, OR = 2.57, P = 0.011) and BISAP score (cut-off 1.5, OR = 1.01, P = 0.038) were independent risk factors for predicting SAP (P < 0.05). By using the area under the receiver operating characteristic (ROC) curve (AUC), MIF (AUC 0.950) demonstrated more excellent discriminative power for predicting SAP than APACHE II (AUC 0.899), BISAP (AUC 0.886), and IL-6 (AUC 0.826). Conclusions Serum MIF is a valuable early marker for predicting the severity of AP based on the RAC.

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