Putrescine as a source of instant energy in the small intestine of the rat

Background and aims—It has been suggested that putrescine acts as a growth factor in the gut, but its exact function in some aspects of cellular metabolism is still in question. The aim of the present work was to identify some functions of putrescine in small bowel metabolism.Animals—Rats (about 80 g), in groups of five, were given either phytohaemagglutinin- or lactalbumin-containing diets, fed ad libitum or were fasted for 48 hours and re-fed for six or twelve hours before being killed.Methods—Uptake of intraperitoneally or intragastrically administered [14C]putrescine and its conversion to succinate by the rat small bowel mucosa was measured. Tissue polyamine and succinate contents were measured by high performance liquid chromatography and amino acid analysis respectively.Results—Uptake of putrescine by the small bowel mucosa from the systemic circulation and conversion of about 30% of this to succinate occurs in the epithelium of the healthy small bowel. Compared with rats given food ad libitum, putrescine uptake was doubled in fasted animals and more than 70% of it was converted to succinate. All these changes returned to control values on refeeding. Using phyto- haemagglutinin induced gut growth as a model, the uptake of putrescine from the systemic circulation by the serosal side of the small intestinal epithelium was increased immediately after growth was stimulated. During phytohaemagglutinin induced growth of the gut, putrescine was converted to succinate in the same proportion as in the healthy small bowel.Conclusions—The experiments identified a novel function for putrescine in gut metabolism: it can be used as an instant energy source when required.

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No Effect of Bolus Glutamine Supplementation on the Postresectional Adaptation of Small Bowel Mucosa in Rats Receiving Chow ad libitum

Digestive Surgery ◽

10.1159/000018844 ◽

2000 ◽

Vol 17 (3) ◽

pp. 256-260 ◽

Cited By ~ 20

Author(s):

Hua Yang ◽

Jörgen Larsson ◽

Johan Permert ◽

Ylva Braaf ◽

Mikael Wirén

Keyword(s):

Small Bowel ◽

Glutamine Supplementation ◽

Small Bowel Mucosa ◽

Ad Libitum ◽

Bowel Mucosa

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Influence of 16,16–Dimethyl Prostaglandin E2on Morphology and Brush Border Enzymes of Small-Bowel Mucosa Differences in Reactivity between Adult and Suckling Rats

Scandinavian Journal of Gastroenterology ◽

10.3109/00365528509092211 ◽

1985 ◽

Vol 20 (sup112) ◽

pp. 41-44 ◽

Cited By ~ 8

Author(s):

A. Baumgartner ◽

H. R. Koelz ◽

M. J. Lentze ◽

F. Halter

Keyword(s):

Small Bowel ◽

Brush Border ◽

Brush Border Enzymes ◽

Suckling Rats ◽

Small Bowel Mucosa ◽

Bowel Mucosa

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Effect of gluten-free diet on the growth and nutritional status of children with coeliac disease

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh0912632r ◽

2009 ◽

Vol 137 (11-12) ◽

pp. 632-637 ◽

Cited By ~ 11

Author(s):

Nedeljko Radlovic ◽

Marija Mladenovic ◽

Zoran Lekovic ◽

Dragana Zivanovic ◽

Radivoj Brdar ◽

...

Keyword(s):

Growth Rate ◽

Small Bowel ◽

Nutritional Status ◽

Coeliac Disease ◽

Gluten Free Diet ◽

Gluten Free ◽

Small Bowel Mucosa ◽

And Gender ◽

Nutritional Status Of Children ◽

Bowel Mucosa

Introduction. Gluten-free diet (GFD) presents the basis of coeliac disease (CD) treatment. If strictly applied, the disorders of the small bowel mucosa and other disease signs rapidly resolve. Objective. The goal of the study was to evaluate the effect of GFD on the growth and nutritional status of children with the classical form of CD. In addition, we analyzed the differences between these parameters with the duration and the patients' compliance with GFD. Methods. The study goals were achieved on a sample of 90 children, 56 female and 34 male, aged 0.5-7.5 (1.53?1.05) years, with the classic CD diagnosed on the basis of typical pathohistological findings of the small bowel mucosa and clinical recovery of patients on GFD. The duration of the patients' follow-up was 1.08-8.75 (3.03?1.14) years, i.e. until the age of 2.5-15 (4.59?1.78) years. The initial and control values of body height (BH) in relation to matched values for age and gender were expressed in percentiles, while the deviation in body weight (BW) for the matched values of height and gender was expressed in percentages. The referent haemoglobin (Hb) rate in blood, as a laboratory indicator of nutritional status in children aged up to 5 years was ?110 g/L, and for those aged above 5 years it was ?115 g/L. Compliance with GFD was based on the pathohistological findings of the small bowel mucosa or determination of tissue transglutaminase. Results. Over the studied period, the effect of GFD was highly significant, both on the increase of BH percentiles (37.62?26.26 vs. 57.22?25.29; p<0.001), and on the decrease of BW deficit 11.58?10.80 vs. 0.89?8.194; p<0.001). After the treatment period, none of the children showed slowed growth rate or BW deficit above 20%, while BW deviation ranging between 10-20% in relation to the referent values was registered in 17 (18.19%) and the excess of over 20% in 2 patients. In 86 (95.56%) patients, control Hb values in blood were normal, while mild anaemia was registered in 4 patients, all compliant with GFD. The difference between the compliant and non-compliant patients with GFD was not detected either in BH percentiles (p=0.586) or in BW percentage deviation as compared to standard values (p=0.516) or in blood Hb values (p=0.445). In addition, differences between the children on GFD lasting over and below 3 years were not detected either in BH percentiles (p=0.915) or in BW deviation percentages in relation to the ideal rate (p=0.476). Conclusion. GFD applied for 1-3 years has a highly significant effect on the growth rate and nutritional status of children with the classical form of CD. Significant differences in these parameters of the disease were not detected between strictly compliant and non-compliant patients on GFD.

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