Grating-based phase-contrast CT (PCCT): histopathological correlation of human liver cirrhosis and hepatocellular carcinoma specimen

AimsTo correlate signal intensities in grating-based phase-contrast CT (PCCT) images obtained at a synchrotron light source and a conventional X-ray source with tissue components in human liver cirrhosis and hepatocellular carcinoma (HCC) specimen.MethodsStudy approval was obtained by the institutional review board. Human specimen of liver cirrhosis and HCC were imaged at experimental grating-based PCCT setups using either a synchrotron radiation source or a conventional X-ray tube. Tissue samples were sectioned and processed for H&E and Elastica van Gieson staining. PCCT and histological images were manually correlated. Depending on morphology and staining characteristics tissue components like fibrosis, HCC, inflammation, connective tissue and necrosis were differentiated and visually correlated with signal intensity in PCCT images using a 5-point Likert scale with normal liver parenchyma as a reference.ResultsGrating-based PCCT images of human cirrhotic liver and HCC specimen showed high soft-tissue contrast allowing correlation with histopathological sections. Signal intensities were similar in both setups independent of the nature of the radiation source. Connective tissue and areas of haemorrhage displayed the highest signal intensities, fibrotic liver tissue the lowest.ConclusionsGrating-based PCCT provides comparable results for the characterisation of human specimen of liver cirrhosis and HCC using either a synchrotron light source or a conventional X-ray tube. Due to its high soft-tissue contrast and its applicability to conventional X-ray tubes grating-based PCCT holds potential for preclinical research and virtual histology applications.

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Comparison of Thy–1 expression in human liver cirrhosis and different models of rat liver injury

Zeitschrift für Gastroenterologie ◽

10.1055/s-2006-931649 ◽

2006 ◽

Vol 44 (01) ◽

Author(s):

T Mansuroglu ◽

J Dudas ◽

B Saile ◽

D Batusic ◽

G Ramadori

Keyword(s):

Liver Cirrhosis ◽

Liver Injury ◽

Rat Liver ◽

Human Liver ◽

Human Liver Cirrhosis

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Design and performance of an X-ray scanning microscope at the Hard X-ray Nanoprobe beamline of NSLS-II

Journal of Synchrotron Radiation ◽

10.1107/s1600577517011183 ◽

2017 ◽

Vol 24 (6) ◽

pp. 1113-1119 ◽

Cited By ~ 42

Author(s):

E. Nazaretski ◽

H. Yan ◽

K. Lauer ◽

N. Bouet ◽

X. Huang ◽

...

Keyword(s):

Material Science ◽

Phase Contrast ◽

Heat Dissipation ◽

Regulation System ◽

Scanning Microscope ◽

National Synchrotron Light Source ◽

X Ray ◽

Synchrotron Light ◽

Differential Phase Contrast ◽

And Performance

A hard X-ray scanning microscope installed at the Hard X-ray Nanoprobe beamline of the National Synchrotron Light Source II has been designed, constructed and commissioned. The microscope relies on a compact, high stiffness, low heat dissipation approach and utilizes two types of nanofocusing optics. It is capable of imaging with ∼15 nm × 15 nm spatial resolution using multilayer Laue lenses and 25 nm × 26 nm resolution using zone plates. Fluorescence, diffraction, absorption, differential phase contrast, ptychography and tomography are available as experimental techniques. The microscope is also equipped with a temperature regulation system which allows the temperature of a sample to be varied in the range between 90 K and 1000 K. The constructed instrument is open for general users and offers its capabilities to the material science, battery research and bioscience communities.

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Hepatocyte Growth Factor mRNA in Human Liver Cirrhosis as Evidenced by in situ Hybridization

Scandinavian Journal of Gastroenterology ◽

10.3109/00365529609052003 ◽

1996 ◽

Vol 31 (9) ◽

pp. 921-927 ◽

Cited By ~ 16

Author(s):

K. Yamaguchi ◽

M. A. Nalesnik ◽

G. K. Michalopoulos

Keyword(s):

Liver Cirrhosis ◽

In Situ Hybridization ◽

Growth Factor ◽

Hepatocyte Growth Factor ◽

Human Liver ◽

Human Liver Cirrhosis ◽

Hepatocyte Growth Factor Mrna ◽

Hepatocyte Growth

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Different protein-binding patterns in the P3 promoter region of the human insulin-like growth factor II gene in the human liver cirrhosis and hepatocellular carcinoma tissues

Journal of Korean Medical Science ◽

10.3346/jkms.1998.13.2.171 ◽

1998 ◽

Vol 13 (2) ◽

pp. 171

Author(s):

J H Seo ◽

K W Kim ◽

B C Park

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cirrhosis ◽

Growth Factor ◽

Protein Binding ◽

Human Insulin ◽

Promoter Region ◽

Human Liver ◽

Insulin Like Growth Factor ◽

Factor Ii ◽

Human Liver Cirrhosis

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Assessment of intraductal carcinoma in situ (DCIS) using grating-based X-ray phase-contrast CT at conventional X-ray sources: An experimental ex-vivo study

PLoS ONE ◽

10.1371/journal.pone.0210291 ◽

2019 ◽

Vol 14 (1) ◽

pp. e0210291 ◽

Cited By ~ 3

Author(s):

Karin Hellerhoff ◽

Lorenz Birnbacher ◽

Anikó Sztrókay-Gaul ◽

Susanne Grandl ◽

Sigrid Auweter ◽

...

Keyword(s):

Carcinoma In Situ ◽

Phase Contrast ◽

Ex Vivo ◽

Intraductal Carcinoma ◽

X Ray ◽

Contrast Ct ◽

Ex Vivo Study

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A feasibility study of X-ray phase-contrast mammographic tomography at the Imaging and Medical beamline of the Australian Synchrotron

Journal of Synchrotron Radiation ◽

10.1107/s160057751501766x ◽

2015 ◽

Vol 22 (6) ◽

pp. 1509-1523 ◽

Cited By ~ 29

Author(s):

Yakov I. Nesterets ◽

Timur E. Gureyev ◽

Sheridan C. Mayo ◽

Andrew W. Stevenson ◽

Darren Thompson ◽

...

Keyword(s):

Phase Contrast ◽

Three Dimensional ◽

High Sensitivity ◽

Contrast Imaging ◽

Detector Resolution ◽

X Ray ◽

Processing Strategies ◽

Characteristics Analysis ◽

Tissue Characteristics ◽

Contrast Ct

Results are presented of a recent experiment at the Imaging and Medical beamline of the Australian Synchrotron intended to contribute to the implementation of low-dose high-sensitivity three-dimensional mammographic phase-contrast imaging, initially at synchrotrons and subsequently in hospitals and medical imaging clinics. The effect of such imaging parameters as X-ray energy, source size, detector resolution, sample-to-detector distance, scanning and data processing strategies in the case of propagation-based phase-contrast computed tomography (CT) have been tested, quantified, evaluated and optimized using a plastic phantom simulating relevant breast-tissue characteristics. Analysis of the data collected using a Hamamatsu CMOS Flat Panel Sensor, with a pixel size of 100 µm, revealed the presence of propagation-based phase contrast and demonstrated significant improvement of the quality of phase-contrast CT imaging compared with conventional (absorption-based) CT, at medically acceptable radiation doses.

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