Hepatocyte Growth Factor mRNA in Human Liver Cirrhosis as Evidenced by in situ Hybridization

1996 ◽  
Vol 31 (9) ◽  
pp. 921-927 ◽  
Author(s):  
K. Yamaguchi ◽  
M. A. Nalesnik ◽  
G. K. Michalopoulos
Keyword(s):  
Liver Cirrhosis ◽  
In Situ Hybridization ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Human Liver ◽  
Human Liver Cirrhosis ◽  
Hepatocyte Growth Factor Mrna ◽  
Hepatocyte Growth

Essential role of HGF (hepatocyte growth factor) in blood formation in Xenopus

Blood ◽  
2004 ◽  
Vol 103 (9) ◽  
pp. 3320-3325 ◽  
Author(s):  
Nobutaka Koibuchi ◽  
Yasufumi Kaneda ◽  
Yoshiaki Taniyama ◽  
Kunio Matsumoto ◽  
Toshikazu Nakamura ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Tyrosine Kinase Domain ◽  
Blood Island ◽  
Significant Difference ◽  
Blood Formation ◽  
Hepatocyte Growth

Abstract In this study, we investigated the role of hepatocyte growth factor (HGF) in blood formation during Xenopus development. First, we examined the gene expression of HGF and its receptor, c-met, by whole-mount in situ hybridization during development. Strong signals of HGF as well as c-met were detected early in the developing ventral mesoderm, which later gives rise to the ventral blood island. Furthermore, to study the role of HGF, we blocked the HGF signaling pathway in Xenopus embryos by using truncated c-met lacking the tyrosine kinase domain. Injection of truncated c-met mRNA resulted in a marked decrease in the number of circulating blood cells. Similar results were obtained using morpholino antisense HGF oligonucleotides. Moreover, we also analyzed the expression of several early primitive blood markers in the blood island of these embryos. RNA in situ analysis revealed a significant reduction (or absence) of stem cell leukemia (SCL), α-globin, and GATA-1 expression, but not GATA-2 expression. In contrast, no significant difference was observed in the levels of expression of early definitive blood markers, SCL, GATA-2, and GATA-3 in the dorsolateral plate, as analyzed by in situ hybridization. Overall, the present study demonstrated that HGF is necessary for primitive hematopoiesis by regulating the expression of SCL. (Blood. 2004;103:3320-3325)

scholarly journals Fibroblast Hepatocyte Growth Factor Promotes Invasion of Human Mammary Ductal Carcinoma In situ

2009 ◽  
Vol 69 (23) ◽  
pp. 9148-9155 ◽  
Author(s):  
Christopher Jedeszko ◽  
Bernadette C. Victor ◽  
Izabela Podgorski ◽  
Bonnie F. Sloane
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Hepatocyte Growth ◽  
Mammary Ductal Carcinoma

Gene therapy for rat liver cirrhosis by naked hepatocyte growth factor gene transffer into liver

2000 ◽  
Vol 118 (4) ◽  
pp. A1014
Author(s):  
Masaharu Takeuchi ◽  
Hiranari Kanemura ◽  
Takahiro Ueki ◽  
Kiyoshi Horiguchi ◽  
Yasukane Asano ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Liver Cirrhosis ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Rat Liver ◽  
Factor Gene ◽  
Growth Factor Gene ◽  
Hepatocyte Growth
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