scholarly journals Effects of culture media on detection of methicillin resistance in Staphylococcus aureus and coagulase negative staphylococci by disc diffusion methods.

1993 ◽  
Vol 46 (5) ◽  
pp. 394-397 ◽  
Author(s):  
L M Milne ◽  
M R Crow ◽  
A G Emptage ◽  
J B Selkon
Keyword(s):  
Staphylococcus Aureus ◽  
Culture Media ◽  
Methicillin Resistance ◽  
Disc Diffusion ◽  
Coagulase Negative Staphylococci

scholarly journals Comparison of culture media for detecting methicillin resistance in Staphylococcus aureus and coagulase negative staphylococci.

1987 ◽  
Vol 40 (10) ◽  
pp. 1178-1181 ◽  
Author(s):  
L M Milne ◽  
G D Curtis ◽  
M Crow ◽  
W A Kraak ◽  
J B Selkon
Keyword(s):  
Staphylococcus Aureus ◽  
Culture Media ◽  
Methicillin Resistance ◽  
Coagulase Negative Staphylococci

scholarly journals Evaluation of a Triplex PCR Assay To Discriminate Staphylococcus aureus from Coagulase-Negative Staphylococci and Determine Methicillin Resistance from Blood Cultures

2002 ◽  
Vol 40 (4) ◽  
pp. 1514-1517 ◽  
Author(s):  
N. Maes ◽  
J. Magdalena ◽  
S. Rottiers ◽  
Y. De Gheldre ◽  
M. J. Struelens
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Blood Cultures ◽  
Pcr Assay ◽  
Coagulase Negative Staphylococci

scholarly journals Bacterial Etiology of Wound Exudates in Tertiary Care Cancer Patients and Antibiogram of the Isolates

2020 ◽  
Vol 13 ◽  
pp. 117863372095207
Author(s):  
Kiran Duwadi ◽  
Sujan Khadka ◽  
Sanjib Adhikari ◽  
Sanjeep Sapkota ◽  
Pabitra Shrestha
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Methicillin Resistance ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Biochemical Tests ◽  
Cross Sectional

Introduction: Patients with malignancies frequently develop infections as a result of surgical procedures and fungating wounds leading to pus formation. This cross-sectional study was conducted to explore the bacteriological spectra of infections of various cancer sites and their antibiotic sensitivity patterns among the patients visiting minor operation theatre (OT) of B.P. Koirala Memorial Cancer Hospital (BPKMCH), Chitwan, Nepal. Methods: Over a period of 3 months from September to November 2018, a total of 183 wound exudates and pus samples were collected and analyzed by standard microbiological procedures. Isolates were identified based on the colony characters, Gram staining and an array of biochemical tests. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion technique according to criteria set by CLSI, 2016. Methicillin resistance in Staphylococcus aureus was tested with the help of cefoxitin using disc diffusion method. Results: Out of the 183 samples, 149 (81.4%) were culture positive. Among 13 different isolates identified, S. aureus (43.0%) was predominant followed by E. coli (14.0%). Higher incidence of bacteria was seen among the males (52.3%), in the age group 51 to 60 years (26.8%) and among the patients undergoing surgical intervention to deal with cancer (34.2%). The prevalence of wound infection was significantly affected by gender, age, and treatment regimen ( P < .01). Out of the total 68 S. aureus isolates, 38 (44.1%) were deemed as Methicillin-resistant Staphylococcus aureus (MRSA). Among the 158 isolates, 85 (53.8%) were multi-drug resistant (MDR). Cefepime was the most effective antibiotic for Gram positive isolates whereas both imipenem and meropenem were found to be equally more effective for Gram negative isolates. Conclusion: This study suggests that patients with malignancies harbor pathogenic bacteria; therefore, prudent use of antibiotics is essential to prevent the emergence of MDR pathogens.

Effects of temperature on the detection of methicillin resistance in Staphylococcus aureus using cefoxitin disc diffusion testing with Iso-Sensitest agar

2009 ◽  
Vol 63 (4) ◽  
pp. 699-703 ◽  
Author(s):  
R. Skov ◽  
R. Smyth ◽  
A. Yusof ◽  
A. Karlsson ◽  
K. Mills ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Disc Diffusion ◽  
Effects Of Temperature

scholarly journals Novel Type XII Staphylococcal Cassette ChromosomemecHarboring a New Cassette Chromosome Recombinase, CcrC2

2015 ◽  
Vol 59 (12) ◽  
pp. 7597-7601 ◽  
Author(s):  
Zhaowei Wu ◽  
Fan Li ◽  
Dongliang Liu ◽  
Huping Xue ◽  
Xin Zhao
Keyword(s):  
United States ◽  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
The United States ◽  
Gene Complex ◽  
Coagulase Negative Staphylococci ◽  
Typing Method ◽  
Content Type ◽  
Staphylococcal Cassette Chromosome ◽  
Sequence Identities

ABSTRACTExcision and integration of staphylococcal cassette chromosomemec(SCCmec) are mediated by cassette chromosome recombinases (Ccr), which play a crucial role in the worldwide spread of methicillin resistance in staphylococci. We report a novelccrgene,ccrC2, in the SCCmecof aStaphylococcus aureusisolate, BA01611, which showed 62.6% to 69.4% sequence identities to all publishedccrC1sequences. A further survey found that theccrC2gene was mainly located among coagulase-negative staphylococci (CoNS) and could be found in staphylococcal isolates from China, the United States, France, and Germany. Theccrgene complex harboring theccrC2gene was designated a type 9 complex, and the SCCmecof BA01611 was considered a novel type and was designated type XII (9C2). This novel SCCmecelement in BA01611 was flanked by a pseudo-SCC element (ΨSCCBA01611) carrying a truncatedccrA1gene. Both individual SCC elements and a composite SCC were excised from the chromosome based on detection of extrachromosomal circular intermediates. We advocate inclusion of the ccrC2gene and type 9ccrgene complex during revision of the SCCmectyping method.

scholarly journals Comparison of mecA gene-based PCR with CLSI cefoxitin and oxacillin disc diffusion methods for detecting methicillin resistance in Staphylococcus aureus clinical isolates

2013 ◽  
Vol 7 (21) ◽  
pp. 2438-2441 ◽  
Author(s):  
Rostami Soodabeh ◽  
Moosavian Mojtaba ◽  
Shoja Saeed ◽  
Torabipour Maryam ◽  
Farshadzadeh Zahra
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Clinical Isolates ◽  
Disc Diffusion

scholarly journals Molecular-genetic Method for Fast Direct Detection of Staphylococcus Aureus and Methicillin Resistance in Blood Cultures and Punctures

Folia Medica ◽  
2019 ◽  
Vol 61 (4) ◽  
pp. 559-565
Author(s):  
Raina T. Gergova ◽  
Virna-Maria S. Tsitou ◽  
Ivan G. Mitov
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Direct Detection ◽  
Methicillin Resistance ◽  
Molecular Genetic ◽  
Empirical Therapy ◽  
Blood Cultures ◽  
Coagulase Negative Staphylococci ◽  
Adequate Treatment ◽  
Genetic Method

Background: Invasive infections caused by methicillin resistant Staphylococcus aureus and coagulase-negative staphylococci (MRSA/MRSCoN) require fast, adequate treatment.&nbsp; The aim of this study was to develop a faster protocol for direct detection of MRSA/MRSCoN in blood cultures and in abscess punctures based on mecA and species specific identification of S. aureus by polymerase-chain reaction (PCR). Materials and methods: We examined 77 growth-positive BACTEC blood cultures and 50 abscess punctures by routine microbiological assay and simultaneous PCR detection of MRSA/MRSCoN. The speci&#64257;city of the PCR was evaluated by using DNA from another 15 microbial species for negative controls. We determined the minimum inhibitory concentration (MIC) of oxacillin, vancomycin, tigecycline, linezolid, levofloxacin, clindamycin, and erythromycin against the S. aureus isolates using the E-test.&nbsp; Results: In the blood cultures, the two methods detected 39.3% of MRSA, and 93.9% of MRCoNS. In the punctures, the PCR assay identified 20.9% of MRSA and 79.2% of MSSA. In the puncture cases, there were three PCR MRSA positive and culture negative samples. Screening for susceptibility to 14 antimicrobial agents demonstrated significantly higher (p<0.05) methicillin resistance in blood culture isolates than in the puncture ones (39.3% and 20.0%, respectively).&nbsp; Conclusion: The new PCR protocol was very fast and specific. It was more sensitive in detecting MRSA from abscess punctures than the routine microbiological techniques. This protocol will speed up the right choice of empirical therapy, which is extremely important for saving patients&rsquo; lives.

scholarly journals Isolation, Characterization and Antibacterial Activity of Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) from Kenyan Ganoderma lucidum

2020 ◽  
pp. 48-57
Author(s):  
Ebrahim Sande ◽  
Danstone Lilechi Baraza ◽  
Selline Ooko ◽  
Peter Kuloba Nyongesa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Ganoderma Lucidum ◽  
Methicillin Resistance ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Aims: To determine the chemical composition and antibacterial activity of Kenyan Ganoderma lucidum. Study Design: Structural determination of the isolated compound was done using spectral evidences and in comparison with literature. The antibacterial properties of the compound was done using disc diffusion method. Place and Duration of Study: Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, between January and November, 2019. Methodology: Sequential extraction of dried samples of Kenyan G. lucidum were done using solvents hexane, ethyl acetate and methanol. Chromatographic separation of hexane extract of Ganoderma lucidum was done using spectroscopic data. The compound was assayed against Escherichia coli, Klebsiella pneumoniae, Methicillin–Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Streptococcus pyogenes. Standard antibiotic namely; ampicillin was used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial activity. Results: From hexane extract of Ganoderma lucidum, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated. Ethylacetate and methanol extracts produced a mixture of complex compounds. Ergosta-5,7,22-triene-3β,14α-diol (22Z) exhibited significant activity against Methicillin-Resistance Staphylococcus aureus (MRSA) (p=0.022) and Streptococcus pyogenes (p = 0.05). The most sensitive microbe was Streptococcus pyogenes. Conclusion: One major compound, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated, characterized and antibacterial activity determined.

Evaluation of phenotypic methods for methicillin resistance characterization in coagulase-negative staphylococci (CNS)

2004 ◽  
Vol 53 (12) ◽  
pp. 1195-1199 ◽  
Author(s):  
Juliana Caierão ◽  
Maiara Musskopf ◽  
Silvana Superti ◽  
Eliane Roesch ◽  
Cícero G Dias ◽  
...  
Keyword(s):  
Nosocomial Infections ◽  
Staphylococcus Epidermidis ◽  
Methicillin Resistance ◽  
Common Species ◽  
Disc Diffusion ◽  
Coagulase Negative Staphylococci ◽  
Resistant Strains ◽  
Staphylococcus Hominis ◽  
Phenotypic Methods ◽  
Microscan System

Coagulase-negative staphylococci (CNS) are the major cause of nosocomial infections. Methicillin-resistant strains are particularly important because they narrow therapeutic options. Detecting methicillin resistance among CNS has been a challenge for years. The objective of this study was to determine the accuracy of an agar screening test (0.6 and 4 μg oxacillin ml−1), disc diffusion and the automated MicroScan system to characterize methicillin resistance among CNS. One hundred and seventy five strains were analysed: 41.1 % Staphylococcus epidermidis and 59.9 % other species; 69.1 % were mecA-positive. The results showed that the methods have optimal correlation with the detection of mecA gene for S. epidermidis, Staphylococcus hominis and Staphylococcus haemolyticus. However, accuracy of the tests is impaired when less common species are analysed. The only 100 % accurate test was agar screening with 4 μg oxacillin ml−1.

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