O10.4 Performance of self-collected penile swabs for the detection ofchlamydia trachomatis,neisseria gonorrhoeae,trichomonas vaginalis, andmycoplasma genitalium

The objective of this study is to assess the predictors and frequency of persistent sexually transmitted infection (STI) positivity in human immunodeficiency virus (HIV)-infected pregnant women treated for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) or Trichomonas vaginalis (TV) infection. We enrolled HIV-infected pregnant women attending their first antenatal care visit and tested them for urogenital CT, NG and TV infection using Xpert® CT/NG and TV assays (Cepheid, Sunnyvale, CA). Those testing positive were treated. Participants either notified partners to seek treatment or were given extra medication to deliver to partners for treatment. Repeat testing was conducted approximately 21 days post-treatment or treatment initiation. Among 427 participants, 172 (40.3%) tested positive for any STI. Of the 136 (79.1%) that returned for repeat testing, 36 (26.5%) tested positive for the same organism: CT = 27 (26.5%), NG = 1 (6.3%), TV = 11 (16.7%). Persistent CT positivity was independently associated with having more than one sex partner in the preceding 12 months (adjusted-prevalence ratio [aPR] = 3.03, 95% CI: 1.44–6.37) and being newly diagnosed with HIV infection during the first antenatal care visit compared to those currently on antiretroviral therapy (aPR = 3.97, 95% CI: 1.09–14.43). Persistent TV positivity was associated with not knowing if a partner sought treatment following STI disclosure (aPR = 12.6, 95% CI: 2.16–73.5) and prior diagnosis of HIV but not currently on antiretroviral therapy. (aPR = 4.14; 95% CI: 1.25–13.79). We identified a high proportion of HIV-infected pregnant women with persistent CT or TV positivity after treatment. To decrease the risk of re-infection, enhanced strategies for partner treatment programmes are needed to improve the effectiveness of STI screening and treatment in pregnancy. The relationship between not being on antiretroviral therapy and persistent STI positivity needs further study.

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Human papillomavirus and Its Association with Other Sexually Transmitted Coinfection among Sexually Active Women from the Northeast of Brazil

Interdisciplinary Perspectives on Infectious Diseases ◽

10.1155/2020/8838317 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Ana Paula Almeida Cunha ◽

Ilka Kassandra Pereira Belfort ◽

Francisco Pedro Belfort Mendes ◽

Gerusinete Rodrigues Bastos dos Santos ◽

Lucas Henrique de Lima Costa ◽

...

Keyword(s):

Chlamydia Trachomatis ◽

Neisseria Gonorrhoeae ◽

Trichomonas Vaginalis ◽

Positive Association ◽

Hpv Infection ◽

Cervical Lesions ◽

Hpv Dna ◽

Sexually Transmitted ◽

Epithelial Lesions ◽

Intraepithelial Lesions

Objective. To verify the association between HPV infection and the presence of coinfections (Chlamydia trachomatis, Trichomonas vaginalis, and Neisseria gonorrhoeae) in women in the state of Maranhão. Methods. HPV-DNA detection was performed by the nested PCR, using the primers PGMY09/11 and GP + 5/GP + 6. For the identification of sexually transmitted agents, conventional PCR was performed using the following primers: KL1/KL2 (Chlamydia trachomatis), TVA5/TVA6 (Trichomonas vaginalis), and HO1/HO3 (Neisseria gonorrhoeae). DNA-HPV positive samples were subjected to automated sequencing for genotyping. Results. Among the 353 women evaluated, 204 (57.8%) had HPV-DNA, of which 140 (68.6%) exhibited HPV/STIs, while 64 (31.4%) had the only HPV. T. vaginalis infection showed a positive association with HPV ( p = 0.003 ). Women without cervical lesions were predominant (327/92.6%); however, the largest number of lesions was reported in women who had HPV/coinfections (18/8.8%). Multiple regression analysis showed that both HPV only and the concomitant presence of HPV/STI were able to indicate the occurrence of epithelial lesions (R = 0.164; R2 = 0.027). Conclusion. The findings suggest that the presence of T. vaginalis can contribute to HPV infection, and HPV/IST association may influence the development of cervical intraepithelial lesions that are precursors of cervical cancer.

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Prevalence of Neisseria gonorrhoeae and Trichomonas vaginalis in Portuguese women of childbearing age

Journal of Obstetrics and Gynaecology ◽

10.1080/01443615.2020.1736014 ◽

2020 ◽

pp. 1-5

Author(s):

Jani Silva ◽

Fátima Cerqueira ◽

Ana Luísa Teixeira ◽

Rui Campainha ◽

José Amorim ◽

...

Keyword(s):

Neisseria Gonorrhoeae ◽

Trichomonas Vaginalis ◽

Women Of Childbearing Age ◽

Childbearing Age

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Flora vaginal e correlação com aspectos citológicos

Revista de Saúde Pública ◽

10.1590/s0034-89101986000600003 ◽

1986 ◽

Vol 20 (6) ◽

pp. 415-420 ◽

Cited By ~ 1

Author(s):

Hygia Maria Nunes Guerreiro ◽

Helenemarie Schaer Barbosa ◽

João Lycio Conceição Filho ◽

Lúcia Maria Tishchenko ◽

Suraia Hagge

Keyword(s):

Candida Albicans ◽

Neisseria Gonorrhoeae ◽

Trichomonas Vaginalis ◽

Gardnerella Vaginalis ◽

Corynebacterium Sp

Com a finalidade de esclarecer a patogenicidade de microorganismos que compõem a microflora vaginal, particularmente nas chamadas vaginites inespecíficas e de determinar o valor da citologia como método para identificação da flora vaginal, foram estudadas 97 pacientes, realizando cultura, exame direto a fresco, Gram e citologia do material cérvico-vaginal. Entre os microorganismos de importância clínica reconhecida, Gardnerella vaginalis foi o mais freqüentemente isolado, 48,4%, seguido de Trichomonas vaginalis, 10,3%, Candida albicans, 7,2% e Neisseria gonorrhoeae, 1,1%. Alterações citológicas indicativas de cervicite e/ou vaginite estiveram presentes na maioria dos casos de G.vaginalis, C.albicans e em todos os casos de T.vaginalis. Foi ressaltada a importância da avaliação semi-quantitativa dos microorganismos nos meios de cultura. Na avaliação da citologia como método diagnóstico para a microflora vaginal, foi observada que este foi o melhor dos métodos utilizados na identificação de T.vaginalis, tendo sido detectado cerca de 50% dos casos de C.albicans. No diagnóstico de G.vaginalis a citologia foi positiva em 48,9% dos casos, destacando-se que dos 31 casos positivos ao exame citológico, oito tiveram culturas negativas para G.vaginalis, embora tenham sido isolados nestes últimos: Haemophilus sp. ou Corynebacterium sp.

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