S107 Effects of Exposure to Cigarette Smoke Condensate on Pneumococcal Gene Expression in Relation to Biofilm Formation

Thorax ◽  
2012 ◽  
Vol 67 (Suppl 2) ◽  
pp. A52.1-A52
Author(s):  
R Cockeran ◽  
J Herbert ◽  
TJ Mitchell ◽  
HC Steel ◽  
ND Mutepe ◽  
...  
2014 ◽  
Vol 14 (1) ◽  
pp. 61 ◽  
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Abdelhabib Semlali ◽  
Kerstin Killer ◽  
Humidah Alanazi ◽  
Witold Chmielewski ◽  
Mahmoud Rouabhia

2020 ◽  
Vol 80 (2) ◽  
pp. 204-209
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Riana Cockeran ◽  
Thèrése Dix-Peek ◽  
Caroline Dickens ◽  
Helen C. Steel ◽  
Ronald Anderson ◽  
...  

2010 ◽  
Vol 138 (4) ◽  
pp. 427-434 ◽  
Author(s):  
Fernanda Brasil Baboni ◽  
Odilon Guariza Filho ◽  
Andréa Novais Moreno ◽  
Edvaldo Antonio Ribeiro Rosa

2018 ◽  
Vol 29 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Yasmin Mohammed Alzayer ◽  
Grace F. Gomez ◽  
George J. Eckert ◽  
John A. Levon ◽  
Richard L. Gregory

2005 ◽  
Vol 86 (1) ◽  
pp. 84-91 ◽  
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Wanda R. Fields ◽  
Randi M. Leonard ◽  
Pamela S. Odom ◽  
Brian K. Nordskog ◽  
Michael W. Ogden ◽  
...  

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Moloko C. Cholo ◽  
Sipho S. M. Rasehlo ◽  
Eudri Venter ◽  
Chantelle Venter ◽  
Ronald Anderson

Background and Objectives. Cigarette smoke (CS) is a major risk factor contributing to the burden of tuberculosis. Little is known, however, about the effects of CS exposure on growth and persistence of Mycobacterium tuberculosis (Mtb) organisms. This issue has been addressed in the current study, which is focused on the effects of cigarette smoke condensate (CSC) on the growth and viability of Mtb planktonic and biofilm-forming cultures. Materials and Methods. The planktonic and biofilm-forming cultures were prepared in Middlebrook 7H9 and Sauton broth media, respectively, using Mtb strain, H37Rv. The effects of CSC at concentrations of 0.05-3.12 mg/L on growth, biofilm formation and structure were evaluated using microplate Alamar Blue assay, spectrophotometric procedure and scanning electron microscopy (SEM), respectively. Involvement of reactive oxygen species in CSC-mediated biofilm formation was investigated by including catalase in biofilm-forming cultures. Results. CSC did not affect the growth of planktonic bacteria, but rather led to a statistically significant increase in biofilm formation at concentrations of 0.4-3.12 mg/L, as well as in the viability of biofilm-forming bacteria at CSC concentrations of 0.2-1.56 mg/L. SEM confirmed an agglomerated biofilm matrix and irregular bacterial morphology in CSC-treated biofilms. Inclusion of catalase caused significant attenuation of CSC-mediated augmentation of biofilm formation by Mtb, implying involvement of oxidative stress. These findings demonstrate that exposure of Mtb to CSC resulted in increased biofilm formation that appeared to be mediated, at least in part, by oxidative stress, while no effect on planktonic cultures was observed. Conclusion. Smoking-related augmentation of biofilm formation by Mtb may contribute to persistence of the pathogen, predisposing to disease reactivation and counteracting the efficacy of antimicrobial chemotherapy.


2012 ◽  
Vol 41 (2) ◽  
pp. 392-395 ◽  
Author(s):  
N. Daphney Mutepe ◽  
Riana Cockeran ◽  
Helen C. Steel ◽  
Annette J. Theron ◽  
Tim J. Mitchell ◽  
...  

Oncogene ◽  
2010 ◽  
Vol 29 (25) ◽  
pp. 3650-3664 ◽  
Author(s):  
F Liu ◽  
J K Killian ◽  
M Yang ◽  
R L Walker ◽  
J A Hong ◽  
...  

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