scholarly journals Multiple cutaneous mast cell tumours in a Boa imperator

2020 ◽  
Vol 8 (1) ◽  
pp. e001040
Author(s):  
Ann-Kathrin Onkels ◽  
Christina Stadler ◽  
Udo Hetzel ◽  
Jana Mueller ◽  
Christiane Herden
Keyword(s):  
Mast Cell ◽  
Subcutaneous Tissue ◽  
Skin Lesions ◽  
Giemsa Stain ◽  
Cell Populations ◽  
Round Cell ◽  
Dense Granules ◽  
The Family ◽  
Cutaneous Mast Cell ◽  
Metachromatic Granules

Mast cell tumours (MCTs) are common in dogs and cats, but are only rarely reported in reptiles. This case documents the clinical and pathomorphological results from a Boa imperator with multiple cutaneous nodules, diagnosed as MCT based on histopathology, immunohistochemistry and electron microscopy approaches. Grossly, there were multifocal, poorly demarcated, mostly ulcerated nodules ~3 cm in diameter on the skin. Histologically, the dermis and the subcutaneous tissue were infiltrated by round cell populations with eosinophilic granules. Toluidine blue and Giemsa stain revealed metachromatic granules. Using immunohistochemistry, some cells exhibited cytoplasmic immunostaining positive for tryptase. Ultrastructurally, variable quantities of intracytoplasmic, spherical and electron-dense granules were also detected. The MCT literature on snakes is scarce, especially for the family Boidae, but MCTs should be considered a differential diagnosis for nodular skin lesions in reptiles.

Mast Cell Tumors and Histiocytomas in Domestic Goats and Diagnostic Utility of CD117/c-Kit and Iba1 Immunohistochemistry

2021 ◽  
Vol 58 (3) ◽  
pp. 508-515
Author(s):  
T. William O’Neill ◽  
Christiane V. Löhr
Keyword(s):  
Mast Cell ◽  
Neuronal Cell ◽  
Normal Lung ◽  
Surgical Removal ◽  
Round Cell ◽  
Diagnostic Challenge ◽  
Mast Cell Tumors ◽  
Neuronal Cell Bodies ◽  
Cutaneous Mast Cell ◽  
Round Cell Tumors

Cutaneous round cell tumors in goats present a diagnostic challenge. In this article, we provide a description of caprine cutaneous mast cell tumors (MCT) and histiocytomas, and report on the validation of anti-human antibodies to CD117/KIT and Iba1 by immunohistochemistry on a range of caprine tissues. Cells immunolabeled for CD117/KIT included resident mast cells in normal lung and skin, interstitial cells of Cajal (intestine), and neuronal cell bodies (brain). Cells immunolabeled for Iba1 included resident macrophages in many tissues including normal lung, dendritic cells (hemolymphatic tissues), Kupffer cells, and microglia. Of 5 cutaneous MCT, only one had metachromasia of cytoplasmic granules; however, neoplastic cells of all 5 MCT had positive immunolabeling for CD117/KIT. The CD117/KIT immunolabeling pattern was predominately focal paranuclear in 3 cases, and cytoplasmic or membranous in 1 case each. Two histiocytomas were identified and had strong positive immunolabeling for Iba1 but not CD117/KIT. All 7 cutaneous round cell tumors described herein occurred in goats less than 4 years of age; the 2 cutaneous histiocytomas were in goats less than 14 months of age. Neither of the cutaneous histiocytomas recurred within 24 months of surgical removal.

scholarly journals Multiple cutaneous mast cell tumors in a pig

2011 ◽  
Vol 23 (6) ◽  
pp. 1222-1225 ◽  
Author(s):  
Jorge Martínez ◽  
Vicente Martínez ◽  
Llorenç Grau-Roma ◽  
Javier López ◽  
Joaquim Segalés
Keyword(s):  
Mast Cell ◽  
Blue Staining ◽  
High Magnification ◽  
Toluidine Blue Staining ◽  
Mast Cell Tumors ◽  
Subcutaneous Tissues ◽  
Poorly Differentiated ◽  
Cutaneous Mast Cell ◽  
Metachromatic Granules ◽  
Current Report

Cutaneous tumors are not usually observed in pigs, and mast cell tumors, in particular, have been rarely described. The current report describes numerous, pink to red, elevated, 1–5-cm nodules in the skin of a 6-month-old slaughter pig. The histopathology of the nodules revealed well-demarcated, but poorly differentiated, populations of round cells that extended from the dermis into subcutaneous tissues. The presence of eosinophils and the observation, at high magnification, of cells with cytoplasmic metachromatic granules, after toluidine blue staining, confirmed the diagnosis of multiple cutaneous mast cell tumors.

scholarly journals Disseminated Cutaneous Mast Cell Tumors with Epitheliotropism and Systemic Mastocytosis in a Domestic Cat

2009 ◽  
Vol 21 (5) ◽  
pp. 710-715 ◽  
Author(s):  
Catherine G. Lamm ◽  
Adam W. Stern ◽  
Amanda J. Smith ◽  
Emily J. Cooper ◽  
Steven W. Ullom ◽  
...  
Keyword(s):  
Mast Cell ◽  
Messenger Rna ◽  
Systemic Mastocytosis ◽  
Present Report ◽  
Skin Lesions ◽  
Domestic Cat ◽  
Neoplastic Cells ◽  
Diagnostic Laboratory ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell

A 15-year-old female Domestic Medium Hair cat presented to the referring veterinarian with a 2-month history of multiple, raised, disseminated, nodular skin lesions. A biopsy of 1 of the lesions was submitted to the Oklahoma Animal Disease Diagnostic Laboratory for evaluation. Histologically, there were multiple dermal nodules composed of sheets of neoplastic round cells. Multifocally, the neoplastic cells formed multiple small clusters of 3–5 cells within the epidermis. Distinct cytoplasmic granules were evident within the neoplastic cells with toluidine blue and Giemsa stains. The neoplastic cells were immunoreactive for c-KIT and lacked immunoreactivity for cluster of differentiation 3 with immunohistochemistry. Based on these findings, multiple epitheliotropic cutaneous mast cell tumors were diagnosed. The cat's health declined rapidly despite aggressive treatment, and the animal was humanely euthanatized. A complete necropsy revealed sheets of similar neoplastic mast cells within the spleen, liver, and individual cells scattered within the bone marrow. Exon 11 of the c-KIT messenger RNA from 1 of the cutaneous masses and the spleen was amplified with reverse transcription polymerase chain reaction, sequenced, and compared with the published c-KIT messenger RNA sequence from fetal cat tissues. The maximum identity was 100% for both tissue samples. To the authors’ knowledge, the present report is the first to describe disseminated cutaneous mast cell tumors with epitheliotropism and systemic mastocytosis in a domestic cat.

scholarly journals Stereology in Grading and Prognosis of Canine Cutaneous Mast Cell Tumors

2021 ◽  
pp. 030098582098513
Author(s):  
Mafalda Casanova ◽  
Sandra Branco ◽  
Inês Berenguer Veiga ◽  
André Barros ◽  
Pedro Faísca
Keyword(s):  
Mast Cell ◽  
Clinical Outcome ◽  
Prognostic Value ◽  
Histological Grade ◽  
Intraobserver Variability ◽  
Low Grade ◽  
High Grade ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Grade Iii

Canine cutaneous mast cell tumors (ccMCTs) are currently graded according to Patnaik and Kiupel grading schemes. The qualitative and semiquantitative parameters applied in these schemes may lead to inter- and intraobserver variability. This study investigates the prognostic value of volume-weighted mean nuclear volume ([Formula: see text]), a stereological estimation that provides information about nuclear size and its variability. [Formula: see text] of 55 ccMCTs was estimated using the “point-sampled intercept” method and compared with histological grade and clinical outcome. The clinical history of dogs treated with surgical excision alone was available for 30 ccMCTs. Statistical differences in [Formula: see text] were found between grade II ([Formula: see text]= 115 ± 29 µm3) and grade III ccMCTs ([Formula: see text]= 197 ± 63 µm3), as well as between low-grade ([Formula: see text]= 113 ± 28 µm3) and high-grade ccMCTs ([Formula: see text]= 184 ± 63 µm3). An optimal cutoff value of [Formula: see text] ≥ 150 µm3 and [Formula: see text] ≥ 140 µm3 was determined for grade III and high-grade ccMCTs, respectively. In terms of prognosis, [Formula: see text] was not able to predict the clinical outcome in 42% of the cases; however, cases with [Formula: see text]<125 µm3 had a favorable outcome. These results indicate that, despite having limited prognostic value when used as a solitary parameter, [Formula: see text] is highly reproducible and is associated with histological grade as well as with benign behavior.

scholarly journals Sinonasal FUS-ERG-Rearranged Ewing’s Sarcoma Mimicking Glomangiopericytoma

2020 ◽  
Vol 13 (3) ◽  
pp. 1393-1396
Author(s):  
Maggie Zhou ◽  
Yen Chen Kevin Ko ◽  
Gregory W. Charville ◽  
Kristen N. Ganjoo
Keyword(s):  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Staining Pattern ◽  
Gene Arrangement ◽  
Round Cell ◽  
The Family ◽  
History Of ◽  
Blue Cell ◽  
Aggressive Tumor ◽  
Membranous Staining

Ewing’s sarcoma is a rare and aggressive tumor that typically arises in the long bones of the extremities. It belongs in the family of small round blue cell tumors and is characterized immunohistochemically by diffuse CD99 expression and molecularly by one of several oncogenic translocations, most commonly t(11;22)(q24;q12) between the <i>EWSR1</i> gene and the <i>FLI1</i> gene. Here we present a rare case of Ewing’s sarcoma in the sinonasal tract with <i>FUS-ERG</i> gene arrangement that was regarded for almost a decade as a sinonasal-type hemangiopericytoma (glomangiopericytoma). This case illustrates the surprisingly prolonged natural history of Ewing’s sarcoma that did not receive therapy for many years and the importance of considering alternative genetic translocations. Our experience suggests that the presence of diffuse CD99 membranous staining pattern in a small blue round cell tumor with morphology typical for Ewing’s sarcoma but FISH negative for <i>EWSR1</i> rearrangement should prompt consideration of <i>FUS-ERG</i> fusion.

scholarly journals RETRACTED ARTICLE: Cutaneous mast cell tumor (Mastocytoma): Cyto- histopathological and haematological investigations

2014 ◽  
Vol 9 (1) ◽  
Author(s):  
Ehsan Hosseini ◽  
Behnam Pedram ◽  
Ali Mohammad Bahrami ◽  
Mohammad Hossein Jaberi Moghaddam ◽  
Javad Javanbakht ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Tumor ◽  
Mast Cell Tumor ◽  
Retracted Article ◽  
Cutaneous Mast Cell
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