An enzyme-linked immunosorbent assay for enzootic bovine leukosis virus antibodies

1980 ◽  
Vol 107 (6) ◽  
pp. 124-126 ◽  
Author(s):  
D. Todd ◽  
B. Adair ◽  
G. Wibberley
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enzootic Bovine Leukosis ◽  
Bovine Leukosis ◽  
Bovine Leukosis Virus

scholarly journals Enzootic bovine Leukosis: development of an indirect enzyme linked immunosorbent assay (I-Elisa) in seroepidemiological studies

1999 ◽  
Vol 30 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Ester Teresa González ◽  
Estela Beatriz Bonzo ◽  
María Gabriela Echeverría ◽  
María Licursi ◽  
María Elisa Etcheverrigaray
Keyword(s):  
Core Protein ◽  
Leukemia Virus ◽  
Test Procedure ◽  
Immunological Response ◽  
Negative Control ◽  
Etiologic Agent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Enzootic Bovine Leukosis ◽  
Bovine Leukosis

Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.

A case study of enzootic bovine leukosis in an Albanian dairy herd based on se-rological and hematological test results

2021 ◽  
Vol 1 (1) ◽  
pp. 28-33
Author(s):  
Majlind Sulçe ◽  
◽  
Anita Koni ◽  
Gerald Muça ◽  
Pëllumb Zalla ◽  
...  
Keyword(s):  
Serological Test ◽  
Dairy Herd ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Scale ◽  
Test Results ◽  
Enzootic Bovine Leukosis ◽  
Cattle Herds ◽  
Bovine Leukosis ◽  
Disease Stages

Enzootic bovine leukosis (EBL) is a notifiable infectious disease with sporadic fre-quency in Albania. EBL is not a zoonotic disease, but it has an important impact on the cattle trade. Diagnosis of this disease can be performed by many diagnostic proce-dures, including agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) tests. In some cases, where persis-tent lymphocytosis is present, cell count can turn useful. This study aimed to evaluate serological and hematological tests' suitability for the diagnosis of EBL and assess the manual and automatic hematological count technique diagnostic parameters com-pared to the serological test. Results suggest that strategic use of hematological and AGID tests in parallel can increase the sensitivity of diagnosis and facilitate the detec-tion of infected animals in different disease stages. Moreover, our results indicate that this approach is feasible in small scale cattle herds size, which properly fits Albanian circumstances

Enzootic bovine leukosis virus in Brazil

1981 ◽  
Vol 13 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Carlos H. Romero ◽  
Cheryl A. Rowe
Keyword(s):  
Enzootic Bovine Leukosis ◽  
Bovine Leukosis ◽  
Bovine Leukosis Virus

scholarly journals Seropositivity and risk factors associated with the presentation of bovine leukosis virus in Sotaquirá, Colombia

2021 ◽  
pp. 2212-2218
Author(s):  
Diana M. Bulla-Castañeda ◽  
Adriana M. Díaz-Anaya ◽  
Diego J. Garcia-Corredor ◽  
Julio C. Tobón-Torreglosa ◽  
Diego Ortiz Ortega ◽  
...  
Keyword(s):  
Risk Factors ◽  
Simple Random Sampling ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Seroprevalence Rate ◽  
High Seroprevalence ◽  
Cross Sectional ◽  
Holstein Breed ◽  
Bovine Leukosis ◽  
Bovine Leukosis Virus

Background and Aim: Enzootic bovine leukosis is a disease economically important to the dairy farming industry worldwide. The virus is of the Deltaretrovirus genus and is primarily transmitted iatrogenically. Most bovines infected with the virus remain asymptomatic with only 5-10% of cattle having lymphomas. This study aimed to determine the seroprevalence of bovine leukosis virus (BLV) in Sotaquirá, Boyacá, Colombia. Materials and Methods: We conducted a descriptive, observational epidemiological cross-sectional study using the simple random sampling method with a sample size of 1000. Blood samples from random bovine were processed using the SERELISA® BLV Ab Mono Blocking indirect enzyme-linked immunosorbent assay kit (Zoetis, USA). The assay had a sensitivity of 97% and a specificity of 98%. The collected data were processed using Epi Info® (Centers for Disease Control and Prevention; Atlanta, Georgia). From the study, we could determine a high seroprevalence of BLV in Sotaquirá. Results: We established a high seroprevalence on BLV in the municipality, with 31.1% apparent seroprevalence and 30.6% real seroprevalence rate. We found that male cattle more than 4 years old (39.4%) and the Ayrshire breed (45.5%) had the highest prevalence rates of the virus. In this study, we could establish statistically significant associations according to breed, age, and gender of the cattle under study. Moreover, we identified the risk factors for BLV infection. We found that in cattle aged <1 year and those older than 4 years of age and those of the Holstein breed, the presentation of infectious bovine rhinotracheitis, mucosal secretions, mastitis, fetal death, the presence of a corral, and the implementation of artificial insemination practices were risk factors for BLV infection. Conclusion: Determining the prevalence of BLV within the herd and identifying the associated risk factors for the disease are fundamental in developing efficient programs for the control and eradication of BLV within herds.

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