scholarly journals Enzootic bovine Leukosis: development of an indirect enzyme linked immunosorbent assay (I-Elisa) in seroepidemiological studies

1999 ◽  
Vol 30 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Ester Teresa González ◽  
Estela Beatriz Bonzo ◽  
María Gabriela Echeverría ◽  
María Licursi ◽  
María Elisa Etcheverrigaray
Keyword(s):  
Core Protein ◽  
Leukemia Virus ◽  
Test Procedure ◽  
Immunological Response ◽  
Negative Control ◽  
Etiologic Agent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Enzootic Bovine Leukosis ◽  
Bovine Leukosis

Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.

scholarly journals Performance assessment of imported ELISA in the serodiagnosis of the enzootic bovine leukosis in herds of Pernambuco state, Brazil

2019 ◽  
Vol 86 ◽  
Author(s):  
Luiz Carlos Fontes Baptista Filho ◽  
Artur Cesar de Carvalho Fernandes ◽  
Tamyres Izarelly Barbosa da Silva ◽  
Taciana Rabelo Ramalho Ramos ◽  
Lúcio Esmeraldo Honório de Melo
Keyword(s):  
Leukemia Virus ◽  
High Sensitivity ◽  
Serum Levels ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Enzootic Bovine Leukosis ◽  
Efficient Test ◽  
Bovine Leukosis ◽  
Low Prevalence

ABSTRACT: Enzootic bovine leukosis (EBL) is an infectious disease of cosmopolitan distribution and chronic character caused by a virus of the Retroviridae family, bovine leukemia virus (BLV). The epidemiological situation of EBL in Brazil has motivated studies to improve its diagnosis, based on the recommended serological techniques: agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA). This study was designed to evaluate the use of imported ELISA for the detection of BLV in dairy herds raised in Pernambuco, Brazil, comparing it to AGID. Blood serum samples from 327 dairy cattle from the state of Pernambuco were tested to AGID and the imported commercial ELISA CHEKIT-Leucose-serum, produced by the IDEXX® laboratory for the diagnosis of EBL. Discarding 25 inconclusive samples from one or both tests, 302 samples were analyzed, being 24.1% positive (73/302) in the AGID and 45% (136/302) in the ELISA, which compared to the AGID, a technique considered standard, presented sensitivity of 98.6%, specificity of 72% and Kappa coefficient of 0.55. The lack of agreement in the diagnostic methods was probably due to the high sensitivity of the ELISA, which makes it possible to detect antibodies even in situations with low serum levels. Although AGID has been shown to be an efficient test so far, in more advanced stages of an EBL control and eradication program, with low prevalence rates, ELISA will present better performance, due to its higher sensitivity, avoiding the permanence of animals that spread the disease in the herds.

Distribution of bovine leukemia virus proviral DNA sequences in tissues of animals with enzootic bovine leukosis

1978 ◽  
Vol 2 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Richard Kettmann ◽  
Arsène Burny ◽  
Yvette Cleuter ◽  
Jacques Ghysdael ◽  
Marc Mammerickx
Keyword(s):  
Dna Sequences ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Enzootic Bovine Leukosis ◽  
Proviral Dna ◽  
Bovine Leukosis

Enzootic Bovine Leukosis and Bovine Leukemia Virus

1983 ◽  
pp. 231-245
Author(s):  
G. Marbaix ◽  
R. Kettmann ◽  
J. Deschamps ◽  
D. Couez ◽  
M. Mammerickx ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Enzootic Bovine Leukosis ◽  
Bovine Leukosis

scholarly journals Bovine leukosis and the possibility to cause cancer in humans: A scientific review

2018 ◽  
Vol 42 (1) ◽  
pp. 52-60
Author(s):  
Huda Hameed Kadhim Alabbody
Keyword(s):  
Bovine Leukemia Virus ◽  
Human Cancer ◽  
Leukemia Virus ◽  
Cell Leukemia ◽  
Scientific Review ◽  
Enzootic Bovine Leukosis ◽  
Human T Cell ◽  
T Cell Leukemia Virus ◽  
Bovine Leukosis ◽  
Control And Eradication

    This review was made to explore the recent multiple studies on enzootic bovine leukosis, focusing on its prevalence, economic impact, the link with public health and the possibility to cause cancer in humans. The causative agent of enzootic bovine leukosis is a virus closely related to human T- cell leukemia virus (HTLV-1). The closeness between the two viruses helps the progress of cancer research in diagnosis and treatment, also the development of a vaccine in both human and veterinary medicine .The enzootic bovine leukosis is widely spread in the continents. The economic loses of enzootic bovine leukosis is related to the lowered productivity of effected cattle, morbidity, mortality and cost of control and eradication. This review proved that bovine leukemia virus is innocent from human cancer infection and there is no proof of virus living in human tissues. But this subject needs a lot of research to know the mechanism of the virus and its affects in cellular content of the organism.

scholarly journals Comparative Analysis of the Serological Reactivity of Individuals with Clinical History of Malaria using Two Different ELISA Tests

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 168
Author(s):  
Yorleydy Ruiz Moreno ◽  
Silvia Tavares Donato ◽  
Fátima Nogueira ◽  
Marcelo Sousa Silva
Keyword(s):  
Clinical History ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Serum Samples ◽  
Serological Tests ◽  
Serological Reactivity ◽  
History Of ◽  
Low Levels ◽  
Antigenic Reactivity

Early diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection.

scholarly journals First Report of Bovine Leukemia Virus Infection in Yaks (Bos mutus) in China

2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Jian-Gang Ma ◽  
Wen-Bin Zheng ◽  
Dong-Hui Zhou ◽  
Si-Yuan Qin ◽  
Ming-Yang Yin ◽  
...  
Keyword(s):  
Risk Factors ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Northwest China ◽  
Gansu Province ◽  
Serum Samples ◽  
First Report ◽  
Elisa Kit ◽  
Bovine Leukosis ◽  
Positive Results

Enzootic bovine leukosis (EBL) is a chronic lymphosarcoma disease of cattle caused by bovine leukemia virus (BLV). No information is available concerning the epidemiology of BLV infection in yaks (Bos mutus). One thousand five hundred and eighty-four serum samples from 610 black yaks and 974 white yaks from Gansu province, northwest China, were collected between April 2013 and March 2014 and tested for BLV antibodies using a commercially available ELISA kit. The overall BLV seroprevalence in yaks was 21.09% (334/1584), with 24.26% (148/610) black yaks and 19.10% (186/974) white yaks yielding positive results. Risk factor analysis indicated that with the exception of breed (OR = 1.36, 95% CI = 1.06–1.73,P<0.05), the age, region, gender, farm, and the numbers of pregnancies were not considered as risk factors for the presence of BLV in yaks included in this study. This is the first report of BLV infection in yaks in China, which provides information for controlling BLV infection in yaks.

Molecular epidemiology of bovine leukemia virus associated with enzootic bovine leukosis in Japan

2011 ◽  
Vol 155 (1) ◽  
pp. 343-348 ◽  
Author(s):  
Keiko Matsumura ◽  
Emi Inoue ◽  
Yoshiaki Osawa ◽  
Katsunori Okazaki
Keyword(s):  
Molecular Epidemiology ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Enzootic Bovine Leukosis ◽  
Bovine Leukosis

A case study of enzootic bovine leukosis in an Albanian dairy herd based on se-rological and hematological test results

2021 ◽  
Vol 1 (1) ◽  
pp. 28-33
Author(s):  
Majlind Sulçe ◽  
◽  
Anita Koni ◽  
Gerald Muça ◽  
Pëllumb Zalla ◽  
...  
Keyword(s):  
Serological Test ◽  
Dairy Herd ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Scale ◽  
Test Results ◽  
Enzootic Bovine Leukosis ◽  
Cattle Herds ◽  
Bovine Leukosis ◽  
Disease Stages

Enzootic bovine leukosis (EBL) is a notifiable infectious disease with sporadic fre-quency in Albania. EBL is not a zoonotic disease, but it has an important impact on the cattle trade. Diagnosis of this disease can be performed by many diagnostic proce-dures, including agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) tests. In some cases, where persis-tent lymphocytosis is present, cell count can turn useful. This study aimed to evaluate serological and hematological tests' suitability for the diagnosis of EBL and assess the manual and automatic hematological count technique diagnostic parameters com-pared to the serological test. Results suggest that strategic use of hematological and AGID tests in parallel can increase the sensitivity of diagnosis and facilitate the detec-tion of infected animals in different disease stages. Moreover, our results indicate that this approach is feasible in small scale cattle herds size, which properly fits Albanian circumstances

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