Preliminary study using an indirect ELISA for the detection of serum antibodies to Alternaria in domestic cats

2005 ◽  
Vol 156 (20) ◽  
pp. 633-635 ◽  
Author(s):  
C. Dye ◽  
I. Peters ◽  
S. Tasker ◽  
S. M. A. Caney ◽  
S. Dye ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Domestic Cats ◽  
Serum Antibodies ◽  
Preliminary Study

scholarly journals A retrospective investigation of canine adenovirus (CAV) infection in adult dogs in Turkey : article

2009 ◽  
Vol 80 (2) ◽  
Author(s):  
S. Gur ◽  
A. Acar
Keyword(s):  
Indirect Elisa ◽  
Adenovirus Infection ◽  
Serum Antibodies ◽  
Serological Investigation ◽  
Retrospective Investigation

Canine adenovirus (CAV) type 1 and 2, respectively, cause infectious canine hepatitis and infectious canine laryngotracheitis in members of the families Canidae and Ursidae worldwide. Both of these infections are acute diseases, especially in young dogs. The aim of this study was to conduct a serological investigation of canine adenovirus infection. For this purpose, serumsamples were collected from native pure-bred Kangal (n = 11), and Akbash dogs (n = 17) and Turkish Greyhounds (n=15) in Eskisehir and Konya provinces. None ofthe dogs were previously vaccinated against CAV types. Indirect ELISA detected 88.2 %, 93.3 % and 100 % prevalences in Akbash, Greyhound and Kangal dogs, respectively. The remainder of the samples (n = 51) were collected at the Afyonkarahisar Municipality Shelter. Fourty-two of these dogs (82.3 %) were detected as seropositive. In total, 82 of 94 dogs (87.2 %) were found to be positive for CAV serum antibodies.

Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins

2002 ◽  
Vol 150 (16) ◽  
pp. 513-518 ◽  
Author(s):  
A. C. German ◽  
E. J. Flach ◽  
G. S. Shankland ◽  
J. Edwards
Keyword(s):  
Aspergillus Fumigatus ◽  
Indirect Elisa ◽  
Serum Antibodies

Molecular Detection of Zoonotic Microsporidia in Domestic Cats in Turkey: A Preliminary Study

2019 ◽  
Vol 64 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Didem Pekmezci ◽  
Gokmen Zafer Pekmezci ◽  
Alparslan Yildirim ◽  
Onder Duzlu ◽  
Abdullah Inci
Keyword(s):  
Molecular Detection ◽  
Domestic Cats ◽  
Preliminary Study

scholarly journals Prevalence and Risk Factors ofToxoplasma gondiiInfection in Domestic Cats from the Tropics of Mexico Using Serological and Molecular Tests

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Virgen J. Castillo-Morales ◽  
Karla Y. Acosta Viana ◽  
Eugenia del S. Guzmán-Marín ◽  
Matilde Jiménez-Coello ◽  
José C. Segura-Correa ◽  
...  
Keyword(s):  
Risk Factors ◽  
Body Condition ◽  
Chronic Infection ◽  
Indirect Elisa ◽  
Body Condition Score ◽  
Domestic Cats ◽  
Molecular Tests ◽  
Condition Score ◽  
Infection Risk Factors ◽  
The Tropics

The aim of this study was to determine the prevalence and risk factors associated withToxoplasma gondiiinfection in domestic cats using an indirect-ELISA (IgM and IgG) and PCR. Samples collected from 220 cats from Merida, Yucatan, Mexico, were analyzed. Cases were reported as acute or chronic. Cases when positive to IgM and IgG and PCR were considered as reactivated chronic infection. Risk factors (sex, age, body condition, diet access to hunting, and number of cats in home) were assessed with a multivariate analysis, 75.5% (166/220) of the cats were IgM and 91.8% (202/220) IgG-seropositive and 79% were PCR-positive (173/220). Number of cats per household and low body condition score were associated with reactivated chronic infection (). It is concluded thatT. gondiiis scattered in the studied population with several periods of reinfection, and therefore an environmental contamination with infecting oocysts exists and there are intrinsic associated factors in cats that increase the risk of becoming infected.

scholarly journals Development of a Blocking Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibodies to O157 Antigen ofEscherichia coli

1998 ◽  
Vol 5 (2) ◽  
pp. 242-246 ◽  
Author(s):  
William Laegreid ◽  
Mark Hoffman ◽  
James Keen ◽  
Robert Elder ◽  
Jimmy Kwang
Keyword(s):  
Escherichia Coli ◽  
Immunosorbent Assay ◽  
Brucella Abortus ◽  
Indirect Elisa ◽  
Bacterial Species ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Method ◽  
Structural Elements ◽  
Serum Antibodies ◽  
E Coli

ABSTRACT The O157 antigen of Escherichia coli shares structural elements with lipopolysaccharide (LPS) antigens of other bacterial species, notably Brucella abortus and Yersinia enterocolitica O9, a fact that confounds the interpretation of assays for anti-O157 antibodies. To address this problem, a blocking enzyme-linked immunosorbent assay (bELISA) was designed with E. coli O157:H7 LPS as the antigen and a monoclonal antibody specific for E. coli O157, designated 13B3, as the competing antibody. The bELISA had equivalent sensitivity to, and significantly higher specificity than, the indirect ELISA (iELISA), detecting anti-O157 antibodies in sera from cattle experimentally inoculated with O157:H7. Only 13% of sera from naive heifers vaccinated for or experimentally infected with B. abortus had increased anti-O157 bELISA titers, while 61% of anti-O157 iELISA titers were increased. The bELISA is a sensitive and specific method for the detection of serum antibodies resulting from exposure to E. coli O157.

The Ultrastructure of Metastatic Human Mammary Carcinoma After Prolonged Estrogen Therapy

1967 ◽  
Vol 25 ◽  
pp. 180-181
Author(s):  
John H.L. Watson ◽  
John L. Swedo ◽  
R.W. Talley
Keyword(s):  
Mammary Carcinoma ◽  
Osmium Tetroxide ◽  
Estrogen Therapy ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Human Mammary Carcinoma ◽  
Surgical Biopsy ◽  
Lead Hydroxide ◽  
Preliminary Study ◽  
Hormonal Therapies

A preliminary study of human mammary carcinoma on the ultrastructural level is reported for a metastatic, subcutaneous nodule, obtained as a surgical biopsy. The patient's tumor had responded favorably to a series of hormonal therapies, including androgens, estrogens, progestins, and corticoids for recurring nodules over eight years. The pertinent nodule was removed from the region of the gluteal maximus, two weeks following stilbestrol therapy. It was about 1.5 cms in diameter, and was located within the dermis. Pieces from it were fixed immediately in cold fixatives: phosphate buffered osmium tetroxide, glutaraldehyde, and paraformaldehyde. Embedment in each case was in Vestopal W. Contrasting was done with combinations of uranyl acetate and lead hydroxide.

Fine structure of early degenerating myofibers in the tibialis anterior of young ‘MDX’ mouse

1988 ◽  
Vol 46 ◽  
pp. 322-323
Author(s):  
H.D. Geissinger ◽  
C.K. McDonald-Taylor
Keyword(s):  
Fine Structure ◽  
Muscle Regeneration ◽  
Tibialis Anterior ◽  
Genetic Defect ◽  
Mdx Mouse ◽  
Mdx Mice ◽  
Histopathological Changes ◽  
Electron Microscopic ◽  
Dystrophic Mouse ◽  
Preliminary Study

A new strain of mice, which had arisen by mutation from a dystrophic mouse colony was designated ‘mdx’, because the genetic defect, which manifests itself in brief periods of muscle destruction followed by episodes of muscle regeneration appears to be X-linked. Further studies of histopathological changes in muscle from ‘mdx’ mice at the light microscopic or electron microscopic levels have been published, but only one preliminary study has been on the tibialis anterior (TA) of ‘mdx’ mice less than four weeks old. Lesions in the ‘mdx’ mice vary between different muscles, and centronucleation of fibers in all muscles studied so far appears to be especially prominent in older mice. Lesions in young ‘mdx’ mice have not been studied extensively, and the results appear to be at variance with one another. The degenerative and regenerative aspects of the lesions in the TA of 23 to 26-day-old ‘mdx’ mice appear to vary quantitatively.

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