Non-host resistance responses of Arabidopsis thaliana to the coffee leaf rust fungus (Hemileia vastatrix)

Botany ◽  
2010 ◽  
Vol 88 (7) ◽  
pp. 621-629 ◽  
Author(s):  
Helena Gil Azinheira ◽  
Maria do Céu Silva ◽  
Pedro Talhinhas ◽  
Clara Medeira ◽  
Isabel Maia ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Rust ◽  
Rust Fungus ◽  
Hemileia Vastatrix ◽  
Callose Deposition ◽  
Mesophyll Cells ◽  
Model Plant ◽  
Heterologous System ◽  
Coffee Leaf Rust ◽  
Model Plant Arabidopsis Thaliana

Leaf rust, caused by Hemileia vastatrix Berk & Broome, is the most destructive fungal disease of coffee. In the absence of a suitable gene validation system in coffee, the objective of this study was to investigate whether the model plant Arabidopsis thaliana (L.) Heynh. may be used as a heterologous system for the molecular dissection of coffee responses to leaf rust. Histological examination of A. thaliana (Col-0) leaves inoculated with H. vastatrix (race II) showed that by 24 h after inoculation (hai), H. vastatrix uredospores differentiated appressoria and penetrated the stomata, but failed to form haustoria. Arabidopsis thaliana cellular resistance responses included hypersensitive-like response (HR) of stomata guard cells together with accumulation of phenolic compounds and callose deposition in walls of epidermal and mesophyll cells. Results indicate that H. vastatrix infection triggered the induction of a set of defence-related genes peaking at 18 and 42 hai. The non-host HR triggered by H. vastatrix in the model plant A. thaliana makes it usable to infer the function of coffee genes involved in pre-haustorial rust resistance.

scholarly journals Agronomic characterization and responses to coffeeleaf rust in coffee progenies resistant to the gall nematode Meloidogyne exigua

2017 ◽  
Vol 38 (2) ◽  
pp. 671
Author(s):  
Ramiro Machado Rezende ◽  
Gladyston Rodrigues Carvalho ◽  
Alex Mendonça de Carvalho ◽  
Antonio Nazareno Mendes ◽  
Sônia Maria Lima Salgado ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Rust Fungus ◽  
Block Design ◽  
Coffee Production ◽  
Hemileia Vastatrix ◽  
Randomized Block Design ◽  
Coffee Beans ◽  
Coffee Leaf Rust ◽  
Specialty Coffee ◽  
Good Potential

The aim of this study was to determine agronomic characteristics and responses to the coffee leaf rust fungus (Hemileia vastatrix Berk. et Br.) in coffee progenies resistant to the gall nematode Meloidogyne exigua. The experiment was conducted in December 2000 at Ouro Verde Farm (FazendaOuro Verde), located in the municipality of Campos Altos – Minas Gerais (MG). Ten F3:4 progenies were evaluated. They were derived from crosses between CIFC 2570 Timor Hybrid selections and Catuaí cultivars known to be resistant to M. exigua. The Catuaí Vermelho IAC 99 cultivar served as a control. A randomized block design with three replicates was used. In total, there were thirty-three plots consisting of eight plants each. The following characteristics were assessed between the harvests of 2011/2012 and 2014/2015: (a) yield expressed as processed coffee bags ha-1; (b) rust incidence and severity; (c) vegetative vigor; (d) percentage of coffee fruits at the “cherry” stage; (e) percentage of floaters; (f) crown diameter; (g) percentage of coffee beans of size sieve 17 and higher; and (h) sensory analysis of the coffee beverage. The results indicated the following: (a) the H514-7-14-2, H514-7-4-5, H493-1-2-2, H514-7-16-3, H514-7-8-11, H518-2-10-1, and H514-5-2-4 progenies were the most productive; (b) all progenies showed promising resistance to coffee leaf rust; (c) all genotypes had the potential for specialty coffee production; (d) the H493-1-2-2 progeny showed resistance both to rust and the nematode, and has good potential for specialty coffee production; and (e) the yields of the H514-7-8-11, H518-2-10-1, H514-5-2-4, H514-7-16-3, H514-7-14-2, H514-7-4-5, and H493-1-2-2 progenies were significantly higher than that of the Catuaí IAC 99 control.

scholarly journals Fused Graphical Lasso Recovers Flowering Time Mutation Genes in Arabidopsis Thaliana

Inventions ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 52
Author(s):  
Rajan Kapoor ◽  
Aniruddha Datta ◽  
Michael Thomson
Keyword(s):  
Arabidopsis Thaliana ◽  
Crop Improvement ◽  
Phenotypic Traits ◽  
The Novel ◽  
Model Plant ◽  
Graphical Lasso ◽  
Analysis Strategy ◽  
Model Plant Arabidopsis Thaliana ◽  
Beneficial Traits ◽  
Delayed Flowering

Conventional breeding approaches that focus on yield under highly favorable nutrient conditions have resulted in reduced genetic and trait diversity in crops. Under the growing threat from climate change, the mining of novel genes in more resilient varieties can help dramatically improve trait improvement efforts. In this work, we propose the use of the joint graphical lasso for discovering genes responsible for desired phenotypic traits. We prove its efficiency by using gene expression data for wild type and delayed flowering mutants for the model plant. Arabidopsis thaliana shows that it recovers the mutation causing genes LNK1 and LNK2. Some novel interactions of these genes were also predicted. Observing the network level changes between two phenotypes can also help develop meaningful biological hypotheses regarding the novel functions of these genes. Now that this data analysis strategy has been validated in a model plant, it can be extended to crop plants to help identify the key genes for beneficial traits for crop improvement.

scholarly journals A fungus-eat-fungus world: Digitopodium, with particular reference to mycoparasites of the coffee leaf rust, Hemileia vastatrix

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Adans A. Colmán ◽  
Harry C. Evans ◽  
Sara S. Salcedo-Sarmiento ◽  
Uwe Braun ◽  
Kifle Belachew-Bekele ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Democratic Republic Of Congo ◽  
Sequence Data ◽  
Molecular Study ◽  
Classical Biological Control ◽  
Coffea Canephora ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust

AbstractDigitopodium hemileiae was described originally in 1930 as Cladosporium hemileiae; growing as a mycoparasite of the coffee leaf rust (CLR), Hemileia vastatrix, in a sample of diseased leaves of Coffea canephora collected in the Democratic Republic of Congo. No cultures from this material exist. More recently, the type material was re-examined and, based on morphological features, considered to be incorrectly placed in Cladosporium. The new genus Digitopodium was erected to accommodate this species. Interest in fungal antagonists of H. vastarix, as potential biocontrol agents of CLR, led to comprehensive surveys for mycoparasites, both in the African centre of origin of the rust, as well as in its South American exotic range. Among the rust specimens from Ethiopia, one was found to be colonized by a fungus congeneric with, and similar to, D. hemileiae. Pure cultures obtained from the Ethiopian material enabled a molecular study and for its phylogenetic position to be elucidated, based on DNA sequence data from the ITS and LSU regions. Molecular data showed that two members of the recently erected genus Hyalocladosporiella (Herpotrichiellaceae: Chaetothyriales) are congeneric with Digitopodium from Ethiopia and morphologically similar to both D. hemileiae and the two Ethiopian isolates. These isolates were found to be morphologically and genetically identical to H. tectonae, described previously from Brazil. Thus, species of Hyalocladosporiella are re-allocated to Digitopodium here; including D. tectonae, and a novel species, D. canescens, recently found in Brazil growing as a mycoparasite of Puccinia thaliae. The potential use of D. hemileiae and D. tectonae for classical biological control of CLR is discussed.

scholarly journals The scope of flavin-dependent reactions and processes in the model plant Arabidopsis thaliana

2021 ◽  
Vol 189 ◽  
pp. 112822
Author(s):  
Reinmar Eggers ◽  
Alexandra Jammer ◽  
Shalinee Jha ◽  
Bianca Kerschbaumer ◽  
Majd Lahham ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Model Plant ◽  
Model Plant Arabidopsis Thaliana ◽  
Plant Arabidopsis Thaliana

scholarly journals Stability of Arabica coffee genotype (Coffea arabica L.) against leaf rust (Hemileia vastatrix)

2021 ◽  
Vol 748 (1) ◽  
pp. 012002
Author(s):  
Sabam Malau ◽  
Albiner Siagian ◽  
Maria Rumondang Sihotang
Keyword(s):  
Leaf Rust ◽  
Block Design ◽  
Research Result ◽  
Dry Season ◽  
Environment Interaction ◽  
Rust Disease ◽  
Hemileia Vastatrix ◽  
Arabica Coffee ◽  
Coffee Leaf Rust ◽  
Rust Severity

Abstract Coffee is now experiencing a serious threat from fungus Hemileia vastatrix which caused epidemic of rust disease in America, Africa, and Asia. As solution, the use of resistant cultivars is the best way. However, interaction between genotype and environment can change the rank of genotypes that shows instability of these genotypes against leaf rust. Purpose of this research was to study stability of genotypes of Arabica coffee against coffee leaf rust. A field experiment was arranged as factorial randomized complete block design with 2 factors (genotypes and climate zones) with three replication. The observed parameters were branch rust incidence, leaf rust incidence, and leaf rust severity. This research result showed significant genotype x environment interaction in all variables. Length of dry season is the most important factor affecting coffee leaf rust because it had the highest correlation coefficient with leaf rust severity (r = 0.662**). Less length of dry season should be the first criteria for selection of coffee farms. The most desired genotype was G7 which performed low leaf rust severity (7.71%) and had a stable resistance indicated by the same leaf rust severity in all environments and 6 SMg. Due to the significant interaction between genotypes and the environment, the genotype to be planted in a region must be tested in that region first.

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