scholarly journals Stability of Arabica coffee genotype (Coffea arabica L.) against leaf rust (Hemileia vastatrix)

2021 ◽  
Vol 748 (1) ◽  
pp. 012002
Author(s):  
Sabam Malau ◽  
Albiner Siagian ◽  
Maria Rumondang Sihotang

Abstract Coffee is now experiencing a serious threat from fungus Hemileia vastatrix which caused epidemic of rust disease in America, Africa, and Asia. As solution, the use of resistant cultivars is the best way. However, interaction between genotype and environment can change the rank of genotypes that shows instability of these genotypes against leaf rust. Purpose of this research was to study stability of genotypes of Arabica coffee against coffee leaf rust. A field experiment was arranged as factorial randomized complete block design with 2 factors (genotypes and climate zones) with three replication. The observed parameters were branch rust incidence, leaf rust incidence, and leaf rust severity. This research result showed significant genotype x environment interaction in all variables. Length of dry season is the most important factor affecting coffee leaf rust because it had the highest correlation coefficient with leaf rust severity (r = 0.662**). Less length of dry season should be the first criteria for selection of coffee farms. The most desired genotype was G7 which performed low leaf rust severity (7.71%) and had a stable resistance indicated by the same leaf rust severity in all environments and 6 SMg. Due to the significant interaction between genotypes and the environment, the genotype to be planted in a region must be tested in that region first.

2022 ◽  
Vol 951 (1) ◽  
pp. 012056
Author(s):  
S Malau ◽  
M R Sihotang

Abstract Coffee leaf rust (CLR) is a pandemic and a serious threat for coffee sustainability in many coffee producing countries. To overcome this CLR, the world’s consensus is to use of resistant cultivars which can be created through coffee breeding program. This research aimed to study genotypic and phenotypic correlations between CLR symptoms of seven arabica coffee (Coffea arabica L.) genotypes that were selected from different districts of North Sumatra Province, Indonesia. This experimental research using a randomized complete block design with three replications was conducted at the experimental garden of the Faculty of Agriculture, Universitas HKBP Nommensen in Medan. The result of this study indicate that leaf rust severity (LRS) had a genotypic component variance of 86.8% which indicated that LRS was controlled more dominantly by plant genetics than other unknown factors. Severity indicated by LRS showed no genotypic and phenotypic correlation with dispersal indicated by branch rust incidence (BRI) and leaf rust incidence (LRI). BRI genotypically correlated with LRI. The results of this study could contribute to resistance coffee breeding for CLR.


2020 ◽  
Vol 8 (1) ◽  
pp. 21
Author(s):  
Sabam Malau

Coffee leaf rust (Hemileia vastatrix) caused large damage on Arabica coffee in Asia, Africa, and America. The resistant genotypes released to farmers in Brazil were developed from the existing cultivars. However, the cultivars of Arabica coffee existing in North Sumatra Province of Indonesia is not yet researched to find resistant genotypes. The goal of this reseach was to determine variability of coffee leaf rust and leaf morphology. In total of 84 genotypes growing in this Province were selected using nested design. Data was analyzed using nested design, correlation, stepwise regression, and hierarchical cluster analysis. This research revealed leaf rust severity had high genotypic variation, low heritability, and high genetic adveance. Ratios of leaf morhology showed moderate to high genotypic variation and heritabilities. Leaf rust severity (y) significantly correlated with both ratio of leaf length with leaf area (x1) and ratio of leaf length with leaf width (x2) with equation y = 2,04 + 62,48x1 3,95x2 with coefficient of multiple correlation R = 0,470**. By using leaf rust severity and these two ratios in cluster analysis, one cluster comprised 18 genotypes with medium leaf rust severity. It could be concluded that resistant genotypes of Arabica coffee might be possible to develop from the existing cultivars. Leaf rust severity might not be used as selection parameter in breeding for leaf rust resistance.


2016 ◽  
Vol 72 (2) ◽  
Author(s):  
Asmini BUDIANI ◽  
I SUSANTI ◽  
Surip MAWARDI ◽  
D A SANTOSO ◽  
. SISWANTO

Summary Leaf rust disease caused by Hemileia vastatrix is considered to be one of the most important diseases on arabica coffee plantation. In order to understand the mechanism underlying resistance of arabica coffee against leaf rust disease, this research was aimed to study expression of β-1,3 glucanase (GLU) and chitinase (CHI) genes in the arabica coffee S1934 and  BLP10 that have been reported respectively as a resistant and susceptible varieties to H. vastatrix. The two varieties were essayed against H. vastatrix, and an RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) using total RNAs  from the S1934 and BLP10, both inoculated with H. vastatrix and uninnoculated was carried out for studying the expression of GLU and CHI. Two primer pairs were designed to amplify the conserved region of GLU and CHI. Amplification products were sequenced and the nucleotide sequences were subjected to BlastX analysis. The result of bioassay confirmed that arabica coffee S1934 was resistant to H. vastatrix, while BLP10 was susceptible.   β-1,3 glucanase was expressed in all of the four samples, the inoculated and uninnoculated S1934, and BLP10 in different degree. S1934 expressed higher GLU compared to BLP10. In the inoculated S1934 the expression of this gene was higher compared to that of the uninoculated one. Expression of CHI was detected only in the S1934, both inoculated and uninoculated. Sequence analysis confirmed that the RT-PCR products were exon regions of genes encoding β-1,3 glucanase dan chitinase respectively. Both of the cDNA fragment have been cloned in E.coli.  Ringkasan Karat daun yang disebabkan oleh jamur Hemileia vastatrix merupakan salah satu penyakit penting pada perkebunan kopi arabika. Untuk memahami mekanisme ketahanan kopi arabika terhadap karat daun, penelitian ini bertujuan untuk mempelajari ekspresi gen β-1,3 glukanase dan kitinase pada varietas kopi arabika S1934 yang dilaporkan tahan karat daun dan varietas BLP10 yang termasuk rentan karat daun. Untuk itu kedua varietas diuji kembali ketahanannya terhadap H. vastatrix melalui bioesai dan dilakukan RT-PCR menggunakan RNA total dari S1934 dan BLP10, baik yang diinokulasi dengan H. vastatrix maupun yang tidak diinokulasi, untuk mempelajari ekspresi gen GLU dan CHI. Dua pasang primer spesifik dirancang untuk mengamplifikasi daerah konservatif kedua gen  tersebut. Hasil amplifikasi disekuen dan dianalisis menggunakan program BlastX. Hasil bioesai mengkonfirmasi bahwa S1934 tahan terhadap H. vastatrix, sedangkan  BLP10 rentan.  β-1,3 glukanase diekspresikan pada kedua varietas, baik yang diinokulasi maupun yang tidak diinokulasi, namun dengan tingkat ekspresi yang sedikit berbeda. Varietas S1934 mengekspresikan β-1,3 glukanase lebih tinggi dibandingkan dengan BLP10. Ekspresi gen tersebut pada S1934 yang diinokulasi lebih tinggi dibandingkan dengan yang tidak diinokulasi. Sedangkan kitinase hanya diekspresikan pada varietas S1934. Hasil sekuensing dan analisis DNA mengkonfirmasi bahwa sekuen hasil RT-PCR merupakan bagian ekson dari gen penyandi β-1,3 glukanase dan kitinase. Kedua fragmen tersebut telah diklon pada E. coli.


2020 ◽  
Vol 142 ◽  
pp. 01006
Author(s):  
Distiana Wulanjari ◽  
Ketut Anom Wijaya ◽  
Muhammad Ghufron Rosyady ◽  
Ali Wafa

The production of arabica coffee in Indonesia is still low because of leaf rust disease suppression caused by Hemileia vastatrix. Basically, it can produce well in the overlapping zone (1000-1500 masl). This potential can be used as basic for intensifying arabica coffee cultivation to the lowland. For anticipation steps to reduce the spread of leaf rust can be done by increasing the production of polyphenol as plant resistance. The addition of mineral element such as silica, boron, iodine, calcium, and potassium can stimulate the synthesis of plant polyphenol. In this research, five minerals above were tested into 7 treatment with single factor RCBD, that is without additional of the mineral element, 150 mg L-1 Si, 300 mg L-1 B, 3000 mg L-1 Ca, 20.000 mg L-1 K, 300 mg L-1 I, and the combination/mix of five mineral elements. The laboratory analysis shows that calcium able to produce the highest polyphenol, but it is not a real difference with silica. The polyphenol that synthesized by Si, I, and K was able to reduce the leaf rust incidence until 78,13%, while Ca can reduce the incidence up to 71,88%.


2017 ◽  
Vol 38 (2) ◽  
pp. 671
Author(s):  
Ramiro Machado Rezende ◽  
Gladyston Rodrigues Carvalho ◽  
Alex Mendonça de Carvalho ◽  
Antonio Nazareno Mendes ◽  
Sônia Maria Lima Salgado ◽  
...  

The aim of this study was to determine agronomic characteristics and responses to the coffee leaf rust fungus (Hemileia vastatrix Berk. et Br.) in coffee progenies resistant to the gall nematode Meloidogyne exigua. The experiment was conducted in December 2000 at Ouro Verde Farm (FazendaOuro Verde), located in the municipality of Campos Altos – Minas Gerais (MG). Ten F3:4 progenies were evaluated. They were derived from crosses between CIFC 2570 Timor Hybrid selections and Catuaí cultivars known to be resistant to M. exigua. The Catuaí Vermelho IAC 99 cultivar served as a control. A randomized block design with three replicates was used. In total, there were thirty-three plots consisting of eight plants each. The following characteristics were assessed between the harvests of 2011/2012 and 2014/2015: (a) yield expressed as processed coffee bags ha-1; (b) rust incidence and severity; (c) vegetative vigor; (d) percentage of coffee fruits at the “cherry” stage; (e) percentage of floaters; (f) crown diameter; (g) percentage of coffee beans of size sieve 17 and higher; and (h) sensory analysis of the coffee beverage. The results indicated the following: (a) the H514-7-14-2, H514-7-4-5, H493-1-2-2, H514-7-16-3, H514-7-8-11, H518-2-10-1, and H514-5-2-4 progenies were the most productive; (b) all progenies showed promising resistance to coffee leaf rust; (c) all genotypes had the potential for specialty coffee production; (d) the H493-1-2-2 progeny showed resistance both to rust and the nematode, and has good potential for specialty coffee production; and (e) the yields of the H514-7-8-11, H518-2-10-1, H514-5-2-4, H514-7-16-3, H514-7-14-2, H514-7-4-5, and H493-1-2-2 progenies were significantly higher than that of the Catuaí IAC 99 control.


2016 ◽  
Vol 72 (2) ◽  
Author(s):  
Asmini BUDIANI ◽  
I SUSANTI ◽  
Surip MAWARDI ◽  
D A SANTOSO ◽  
. SISWANTO

Summary Leaf rust disease caused by Hemileia vastatrix is considered to be one of the most important diseases on arabica coffee plantation. In order to understand the mechanism underlying resistance of arabica coffee against leaf rust disease, this research was aimed to study expression of β-1,3 glucanase (GLU) and chitinase (CHI) genes in the arabica coffee S1934 and  BLP10 that have been reported respectively as a resistant and susceptible varieties to H. vastatrix. The two varieties were essayed against H. vastatrix, and an RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) using total RNAs  from the S1934 and BLP10, both inoculated with H. vastatrix and uninnoculated was carried out for studying the expression of GLU and CHI. Two primer pairs were designed to amplify the conserved region of GLU and CHI. Amplification products were sequenced and the nucleotide sequences were subjected to BlastX analysis. The result of bioassay confirmed that arabica coffee S1934 was resistant to H. vastatrix, while BLP10 was susceptible.   β-1,3 glucanase was expressed in all of the four samples, the inoculated and uninnoculated S1934, and BLP10 in different degree. S1934 expressed higher GLU compared to BLP10. In the inoculated S1934 the expression of this gene was higher compared to that of the uninoculated one. Expression of CHI was detected only in the S1934, both inoculated and uninoculated. Sequence analysis confirmed that the RT-PCR products were exon regions of genes encoding β-1,3 glucanase dan chitinase respectively. Both of the cDNA fragment have been cloned in E.coli.  Ringkasan Karat daun yang disebabkan oleh jamur Hemileia vastatrix merupakan salah satu penyakit penting pada perkebunan kopi arabika. Untuk memahami mekanisme ketahanan kopi arabika terhadap karat daun, penelitian ini bertujuan untuk mempelajari ekspresi gen β-1,3 glukanase dan kitinase pada varietas kopi arabika S1934 yang dilaporkan tahan karat daun dan varietas BLP10 yang termasuk rentan karat daun. Untuk itu kedua varietas diuji kembali ketahanannya terhadap H. vastatrix melalui bioesai dan dilakukan RT-PCR menggunakan RNA total dari S1934 dan BLP10, baik yang diinokulasi dengan H. vastatrix maupun yang tidak diinokulasi, untuk mempelajari ekspresi gen GLU dan CHI. Dua pasang primer spesifik dirancang untuk mengamplifikasi daerah konservatif kedua gen  tersebut. Hasil amplifikasi disekuen dan dianalisis menggunakan program BlastX. Hasil bioesai mengkonfirmasi bahwa S1934 tahan terhadap H. vastatrix, sedangkan  BLP10 rentan.  β-1,3 glukanase diekspresikan pada kedua varietas, baik yang diinokulasi maupun yang tidak diinokulasi, namun dengan tingkat ekspresi yang sedikit berbeda. Varietas S1934 mengekspresikan β-1,3 glukanase lebih tinggi dibandingkan dengan BLP10. Ekspresi gen tersebut pada S1934 yang diinokulasi lebih tinggi dibandingkan dengan yang tidak diinokulasi. Sedangkan kitinase hanya diekspresikan pada varietas S1934. Hasil sekuensing dan analisis DNA mengkonfirmasi bahwa sekuen hasil RT-PCR merupakan bagian ekson dari gen penyandi β-1,3 glukanase dan kitinase. Kedua fragmen tersebut telah diklon pada E. coli.


Author(s):  
Riza Kusuma Widia Siska ◽  
Lahmuddin Lubis ◽  
Lisnawati Lisnawati

Kabupaten Mandailing Natal merupakan daerah penghasil kopi di Sumatera Utara. Salah satu factor pembatas produksi kopi di kabupaten ini adalah adanya serangan penyakit karat daun yang disebabkan oleh Hemileia vastatrix. Namun informasi sebaran dan keparahan penyakit karat daun kopi di kabupaten ini belum ada.Padaha linformasi ini diperlukan untuk menentukan strategi pengendalian yang tepat. Penelitian ini bertujuan untuk mengetahui keparahan penyakit dan pemetaan sebaranpenyakit karat daun kopi pada perkebunan kopi rakyat di Kabupaten Mandailing Natal. Penelitian dilaksanakan dengan metode purposive random sampling (PRS) di tiga kecamatan masing-masing Kecamatan Pakantan, Puncak Sorik Marapi dan Ulu Pungkut dengan lokasi geografis 0° 10° – 1° 50° LU dan 98° 50° – 100° 10° BT dengan ketinggian tempatantara 861-1353 meter diatas permukaan laut. Hasil penelitian menunjukkan penyakit karat daun kopi telah tersebar pada letak geografis 0°10°-1°50°LU dan 98°50°-100°10°BT dengan keparahan penyakit karat daun kopi tertinggi yaitu 45% terdapat di Desa Huta Namale, Kecamatan Puncak Sorik Marapi, sebaliknya keparahan penyakit karat daun kopi yang terendah terdapatdi Desa Huta Julu, Kecamatan Pakantan yaitu sebesar1%. Faktor suhu, ketinggian tempat dan jarak tanam berkorelasi sangat rendah terhadap tingkat kejadian dan keparahan penyakit karat daun kopi. Mandailing Natal Regency is a coffee-producing area in North Sumatra. One of the limiting factors for coffee production in this district is the attack of coffee leaf rust disease caused by Hemileia vastatrix. The information on the distribution and severity of coffee leaf rust disease in this district is needed to determine its control strategy. This study aims to determine the severity of the disease and the mapping of the spread of coffee leaf rust disease on people's coffee plantations in Mandailing Natal District. The study was carried out by purposive random sampling (PRS) method in three sub-districts of Pakantan Subdistrict, Sorik Marapi and Ulu Pungkut Peak with a geographical location of 0 ° 10 ° - 1 ° 50 ° N and 98 ° 50 ° - 100 ° 10 ° with the altitude between 861-1353 meters above sea level. The results showed that the coffee leaf rust disease was spread over a geographical location of 0 ° 10 ° -1 ° 50 ° N and 98 ° 50 ° -100 ° 10 ° East with the highest severity of coffee leaf rust disease, 45% in Huta Namale Village, District Sorik Marapi Peak, on the contrary the lowest severity of coffee leaf rust disease is in Huta Julu Village, Pakantan District, which is equal to 1%. Temperature, altitude, and spacing factors are very low correlated with the incidence and severity of coffee leaf rust disease.


2020 ◽  
pp. 517-529
Author(s):  
Leonel Alvarado-Huamán ◽  
Ricardo Roberto Borjas-Ventura ◽  
Viviana Castro-Cépero ◽  
Leslie García-Nieves ◽  
Jorge Jimenez-Dávalos ◽  
...  

Introduction. Coffee (Coffea arabica L.) is an important crop in producing countries like Peru, where approximately two million families depend on its production, distribution, and marketing. But in recent years, climate change has increased the presence of coffee leaf rust - CLR (H. vastatrix), a disease that has decreased Peruvian production by up to 27%. Objective. Monitor the severity of CLR in different genotypes of coffee cv. Typica from April-2017 to March-2018. Material and methods. The experiment was carried out in the coffee germplasm bank at the Development Regional Institute (IRD)-Selva of the Universidad Nacional Agraria La Molina. The severity and the area under the disease progress curve (AUDPC) in the lower, middle, and upper part of coffee trees were quantified. Result. There was high severity and AUDPC in the dry season (low precipitation) compared to the rainy season (high precipitation). Severity and AUDPC gradually decreased from the bottom to the top of the plant (lower>middle>upper). Also, UNACAF-24A, UNACAF-16, UNACAF-158, and UNACAF-162 genotypes stood out by showing lowest severity (0-1.1 %) and AUDPC (0-714) in the experiment. Conclusion. In this experiment, the genotypes in dry season presented high peaks of severity and UDPC of CLR, meanwhile, in rainy season the presence of CLR was lower. However, in both seasons, severity and AUDPC gradually decreased from the lower third to the upper thirf of the plant. Finally, UNACAF-24A, UNACAF-16, UNACAF-158, and UNACAF-162 presented the lowest degrees of severity of CLR.


2019 ◽  
Vol 6 (2) ◽  
pp. 69
Author(s):  
Sabam Malau

<p>Coffee leaf rust disease (<em>Hemileia vastatrix</em>) causes large damage to Arabica coffee plantation in Asia, Africa, and America.  In Indonesia, particularly in North Sumatra, the resistance level of Arabica coffee genotypes is still unknown. The objective of this research was to determine the resistance variability of Arabica coffee genotypes to leaf rust disease and its relation to leaf morphology.  A total of 84 genotypes grown in North Sumatra were selected in November 2015 and 2016, and December 2017 using a nested design.  Data were analyzed using nested design, correlation, stepwise regression, and cluster hierarchy analysis. The result showed that the G56 genotype performed the most resistant to leaf rust disease, with a severity of 5.21%.  The severity of leaf rust disease has high genotypic variation, low heritability, and high genetic advance.  Leaf morphological ratios showed moderate to high genotypic variation and heritability.  The severity of leaf rust (y) significantly correlated with the ratio of leaf length to leaf area (x<sub>1</sub>) and the ratio of leaf length to leaf width (x<sub>2</sub>) with the equation y = 2.04 + 62.48x<sub>1 </sub>- 3.95x<sub>2,</sub> and multiple correlation coefficients R = 0.470 **. By using the leaf rust severity and the two ratios in the cluster analysis, these 84 genotypes were grouped into five clusters.  The result showed that several Arabica coffee genotypes with a high level of resistance to leaf rust disease are potential to be further developed.</p>


1993 ◽  
Vol 33 (1) ◽  
pp. 97 ◽  
Author(s):  
TV Price ◽  
R Gross ◽  
Wey J Ho ◽  
CF Osborne

Using disease diagrams, visual assessments of coffee leaf rust from Papua New Guinea overestimated severity by >2 times when >20% leaf area was rusted. Nevertheless, a significant linear relationship was established between visual and planimeter assessments of coffee rust severity. Colour image analysis, using a Matrox digitiser, was more accurate than visual estimates or black and white imaging systems in discriminating between rusted and healthy leaf tissues in colour transparency photographs of rusted coffee leaves. Electronic flash photography of rusted coffee leaves produced images with reflections from the waxy leaf surface which made it difficult to discriminate between healthy tissues and the background.


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