Tomato bushy stunt virus from Prunus avium. II. Serological typing and the characterization of antibody types and activities

1968 ◽  
Vol 46 (3) ◽  
pp. 229-233 ◽  
Author(s):  
Wayne R. Allen
Keyword(s):  
Type Strain ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Tomato Bushy Stunt Virus ◽  
Cross Reactivity ◽  
Primary Response ◽  
Maximum Value ◽  
Serological Typing ◽  
Maximum Titer

Serological comparisons between the type strain of tomato bushy stunt virus and a strain from sweet cherry demonstrated that the degree of relationship was highly dependent upon the time of bleeding during the primary response. Cross-reactivity decreased after injection until just before the maximum titer was reached, and then gradually increased until the maximum value was attained. The increase in cross-reactivity was due, mainly, to a decline in synthesis and (or) release of specific antibodies. Sera fractionation revealed specific and cross-reactive antibodies among both light and heavy globulins. The cross-reactivity of light antibodies increased from the time of injection, but it remained nearly constant for heavy antibodies. Immunoelectrophoresis associated the light and heavy antibodies with IgG and IgM globulins, respectively.

Biochemical and phenotypic characterization of sweet cherry (Prunus avium L.) cultivars with induced surface pitting

2021 ◽  
Vol 175 ◽  
pp. 111494
Author(s):  
Excequel Ponce ◽  
Blanca Alzola ◽  
Natalia Cáceres ◽  
Madeline Gas ◽  
Catalina Ferreira ◽  
...  
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Phenotypic Characterization ◽  
Prunus Avium L

scholarly journals Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 57 ◽  
Author(s):  
Kadriye Çağlayan ◽  
Vahid Roumi ◽  
Mona Gazel ◽  
Eminur Elçi ◽  
Mehtap Acioğlu ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Open Reading Frames ◽  
Cherry Tree ◽  
Coding Regions ◽  
Sweet Cherries ◽  
Identification And Characterization ◽  
Reading Frames

High throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5′ UTR and 3′ UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, RdRp, TGB1, TGB2, TGB3 and CP share amino acid identities ranging from 43–53%, 44–60%, 39–43%, 38–44% and 45–50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies.

TOMATO BUSHY STUNT VIRUS FROM PRUNUS AVIUM L.: I. FIELD STUDIES AND VIRUS CHARACTERIZATION

1967 ◽  
Vol 45 (12) ◽  
pp. 2375-2383 ◽  
Author(s):  
Wayne R. Allen ◽  
T. R. Davidson
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Field Studies ◽  
Tomato Bushy Stunt Virus ◽  
Veinal Necrosis ◽  
Fruit Flesh ◽  
Particle Size And Morphology ◽  
Size And Morphology ◽  
First Time ◽  
Prunus Avium L

Tomato bushy stunt virus was isolated apparently for the first time from naturally infected rosaceous plants. The virus was recovered from leaves, flower parts, seeds, and fruit flesh of sweet cherry trees growing in Ontario. Characteristic symptoms associated with the presence of the virus were pitted fruit flesh, veinal necrosis, leaf twisting, and severe shoot stunt. Orchard spread was not proved and the virus was not recovered from the soil or from orchard weeds. Results from tests on transmission by pollen and by Olpidium zoospores were also negative. The cherry isolate was distinguishable from the type strain in Datura and tomato. Both viruses appeared to have the same particle size and morphology, but differed slightly in their rates of sedimentation and in antigenic composition.

scholarly journals Optimization and characterization of Royal Dawn cherry (Prunus avium) phenolics extraction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lisard Iglesias-Carres ◽  
Anna Mas-Capdevila ◽  
Francisca Isabel Bravo ◽  
Miquel Mulero ◽  
Begoña Muguerza ◽  
...  
Keyword(s):  
Food Industry ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Specific Method ◽  
Phenolic Composition ◽  
Phenolic Profile ◽  
Beneficial Effects ◽  
Sweet Cherries ◽  
Precise Characterization

AbstractTo correlate the beneficial effects of cherry consumption with their phenolic composition, a full and precise characterization is required. However, there is not a specific method to fully extract all phenolic compounds from sweet cherries. Thus, this study aimed to optimize the extraction of sweet cherry phenolics by response surface methodology and fully characterize the phenolic profile of Royal Dawn sweet cherries by HPLC-ESI-MS/MS. Extraction conditions were evaluated and optimized to 55 °C, MeOH 72%, 12 mL/g in two extraction steps. Royal Dawn sweet cherries presented rutin as the predominant phenolic compound, unlike most sweet cherry varieties. Additionally, ethanol was evaluated as a replacement solvent, obtaining lower extraction rates, especially for anthocyanins. However, in terms of total amounts, non-anthocyanin compounds were similarly extracted. The developed methodology was fast and can be routinely used in the evaluation of the phenolic profile of sweet cherries and to produce phenolic-rich extracts for the food industry.

scholarly journals Fruit Quality Characterization of New Sweet Cherry Cultivars as a Good Source of Bioactive Phenolic Compounds with Antioxidant and Neuroprotective Potential

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 677
Author(s):  
Fabiana Antognoni ◽  
Giulia Potente ◽  
Roberto Mandrioli ◽  
Cristina Angeloni ◽  
Michela Freschi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Total Anthocyanin ◽  
Sweet Cherries ◽  
Array Detection ◽  
Sweet Cherry Cultivars ◽  
New Cultivars

Sweet cherries (Prunus avium L.) are highly appreciated fruits for their taste, color, nutritional value, and beneficial health effects. In this work, seven new cultivars of sweet cherry were investigated for their main quality traits and nutraceutical value. The phytochemical profile of three classes of phenolic compounds and the antioxidant activity of the new cultivars were investigated through high-performance liquid chromatography with diode array detection (HPLC-DAD) and spectrophotometric assays, respectively, and compared with those of commonly commercialized cultivars. Cyanidine-3-O-rutinoside was the main anthocyanin in all genotypes, and its levels in some new cultivars were about three-fold higher than in commercial ones. The ORAC-assayed antioxidant capacity was positively correlated with the total anthocyanin index. The nutraceutical value of the new cultivars was investigated in terms of antioxidant/neuroprotective capacity in neuron-like SH-SY5Y cells. Results demonstrated that the new cultivars were more effective in counteracting oxidative stress and were also able to upregulate brain-derived neurotrophic factor (BDNF), a pro-survival neurotrophin, suggesting their potential pleiotropic role in counteracting neurodegenerations.

scholarly journals Characterization of the S-RNase promoters from sweet cherry (Prunus avium L.)

2003 ◽  
Vol 78 (2) ◽  
pp. 191-194 ◽  
Author(s):  
Takako Ishizaka ◽  
Hideaki Nakano ◽  
Takashi Suzuki ◽  
Hiroyasu Kitashiba
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Prunus Avium L
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