In vitro studies of floral shoot apices of Pharbitis nil

1970 ◽  
Vol 48 (7) ◽  
pp. 1355-1358 ◽  
Author(s):  
D. S. Bhar
Keyword(s):  
Dark Period ◽  
Floral Organ ◽  
In Vitro Studies ◽  
Pharbitis Nil ◽  
Shoot Apices ◽  
Intact Plants ◽  
Full Complement

Shoot apices of Pharbitis nil were excised and cultured in vitro at different intervals after the intact plants were photoperiodically induced. The induced apices excised immediately after and up to 24 h after an inductive dark period, grew vegetatively for at least 4 weeks in culture. The apices excised and cultured from intact plants at 36 and 48 h after induction initiated a full complement of floral-organ primordia when observed after 4 weeks of growth.

Vegetative and reproductive development of shoot apices of Pharbitis nil as influenced by photoperiodism

1969 ◽  
Vol 47 (9) ◽  
pp. 1403-1406 ◽  
Author(s):  
D. S. Bhar ◽  
N. W. Radforth
Keyword(s):  
Mitotic Activity ◽  
Dark Period ◽  
Pollen Grains ◽  
Reproductive Development ◽  
Epidermal Cells ◽  
Floral Transition ◽  
Pharbitis Nil ◽  
Shoot Apices ◽  
The Third

In shoot apices of Pharbitis nil, cytohistological zonation is not clearly delineated at germination. With increase in age, zonation develops and the granular appearance of the cytoplasm disappears. After induction by a single 16-hour dark period the first recognizable sign of floral transition becomes evident on the second day, with increase in the mitotic activity of the apex. On the third and fourth days, the sepals are initiated and the petals and stamens are initiated simultaneously on the fifth day. On the sixth day the carpel primordia originate. Between the 11th and 12th days after the inductive dark period, the pollen grains develop and the style extends. Between the 19th and 20th days the epidermal cells of the stigma become secretory.

scholarly journals The effect of photoperiodic treatments on mitotic activity in Pharbitic nil Chois

10.5586/1201 ◽  
2014 ◽  
Vol 60 (1-2) ◽  
pp. 133-138
Author(s):  
Joanna Czaplewska ◽  
Jan Kopcewicz
Keyword(s):  
Mitotic Activity ◽  
Mitotic Index ◽  
Dark Period ◽  
Continuous Light ◽  
The State ◽  
Pharbitis Nil ◽  
Shoot Apices ◽  
Inductive Cycle ◽  
Short Day ◽  
Inductive Photoperiod

The short-day plant, <i>Pharbitis nil</i>, requires only a single inductive cycle with a 16-hour dark period for flowering. The mitotic activity in the shoot apices was studied directly after the termination of the inductive photoperiod. A pronounced rise in the mitotic index was found in the 2nd and the 8th-14th hours. Control plants grown under noninductive conditions (continuous light, a light interruption in the middle of the dark period) did not flower and did not show an increased mitotic index. The increased mitotic activity in the shoot apices of <i>Pharbitis</i> seems to be causally connected with the phytochrome-controlled entry of the plants into the state of generative induction.

The Behaviour of Shoot Apices of Lolium temulentum in vitro as the Basis of an Assay System for Florigenic Extracts

1993 ◽  
Vol 20 (3) ◽  
pp. 337 ◽  
Author(s):  
RW King ◽  
C Blundell ◽  
LT Evans
Keyword(s):  
Gibberellic Acid ◽  
Growth Medium ◽  
High Irradiance ◽  
Plant Age ◽  
Assay System ◽  
Shoot Apices ◽  
Long Day ◽  
Intact Plants ◽  
Short Days

Previous experiments have shown that shoot apices excised from plants of L. temulentum that had been exposed to one long day (LD) could form inflorescence primordia in vitro if gibberellic acid (GA3) was present in the medium whereas apices from plants in short days (SD) could not. We show here that apices from older plants grown under high irradiance can undergo inflorescence differentiation in vitro after one LD even in the absence of GA3, as can apices from plants in SD if GA3 is present in the medium. For apices excised from both SD and one LD plants, the order of effectiveness of gibberellins for inflorescence induction was 2,2-dimethyl GA4 > GA5 > GA3 > GA1, in agreement with their ranking for effectiveness on intact plants. Application of GA3 to leaves before apex excision can substitute for GA3 in the growth medium. The presence of GA3 in the medium is not required until 4-6 days after excision from plants given one LD, and appears to be necessary for differentiation beyond the spikelet primordia stage. Three hypotheses concerning the relation between GAs and the LD stimulus to flowering, as affected by plant age, are considered.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production

Determination of floral organ type in cultured Silene shoot apices

1993 ◽  
Vol 89 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Iain Simon Donnison ◽  
Dennis Francis
Keyword(s):  
Floral Organ ◽  
Shoot Apices ◽  
Organ Type

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

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