Inoculation of a nonhost tissue culture with Cronartium ribicola

1971 ◽  
Vol 49 (6) ◽  
pp. 881-882 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Tissue Culture ◽  
Pseudotsuga Menziesii ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola

Cultured tissues from Pseudotsuga menziesii (Mirb.) Franco were successfully inoculated with Cronartium ribicola J. C. Fisch. ex Rabenh. Typical intracellular haustoria were formed 60 days after inoculation. Invaded nonhost tissues were necrotic within 90 days, and dead within 120 days. Tissue cultures of either Pinus monticola Dougl. or P. menziesii had an attractant effect on nearby rust mycelium.

Histopathology and Cytology of Cronartium ribicola in Tissue Cultures of Pinus monticola

1971 ◽  
Vol 61 (7) ◽  
pp. 773 ◽  
Author(s):  
A. E. Harvey
Keyword(s):  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola

Ultrastructure of tissue cultures of Pinus monticola infected by Cronartium ribicola. I. Prepenetration host changes

1975 ◽  
Vol 5 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Jane Robb ◽  
A.E. Harvey ◽  
Michael Shaw
Keyword(s):  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola

The relative susceptibility of needle- and stem-derived white pine tissue cultures to artificial inoculation with mycelium of Cronartium ribicola

1969 ◽  
Vol 47 (11) ◽  
pp. 1789-1790 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Rust Fungus ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Relative Susceptibility ◽  
Blister Rust ◽  
Leaf Mesophyll ◽  
Leaf Tissues ◽  
Artificial Inoculations

Tissue cultures of Pinus monticola Dougl. derived from stem cortex and leaf tissues were found susceptible to artificial inoculations with mycelium from the blister rust fungus (Cronartium ribicola Fisch. ex Rabenh.). Tissue cultures from leaf mesophyll grew slower and were colonized more rapidly by this fungus than those derived from stem cortex.

Ultrastructure of hyphal walls and septa of Cronartium ribicola on tissue cultures of Pinus monticola

1973 ◽  
Vol 51 (12) ◽  
pp. 2301-2305 ◽  
Author(s):  
Jane Robb ◽  
A. E. Harvey ◽  
Michael Shaw
Keyword(s):  
Electron Microscopy ◽  
Rust Fungus ◽  
Callus Cultures ◽  
Fungal Mycelium ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
Host Parasite ◽  
Lateral Walls

Colonies of the rust fungus Cronartium ribicola J. C. Fischer were grown axenically and then transferred so that they lay immediately next to callus cultures of the pine host (Pinus monticola Dougl.). Twelve days after the establishment of this association samples of the fungal mycelium taken 2 mm away from the pine callus and at the host–parasite interface were fixed for electron microscopy. Differences were found in the appearance and thickness of the lateral walls and extracellular coatings depending on the proximity of the fungus to the host–parasite interface. Septa were intermediate in structure between the Ascomycete and dolipore types. The origin of the extracellular sheath is discussed.

Production of the nutritional requirements for growth of Cronartium ribicola by a nonhost species

1971 ◽  
Vol 49 (8) ◽  
pp. 1517-1519 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Pseudotsuga Menziesii ◽  
Rust Fungus ◽  
Molecular Size ◽  
Nutritional Requirements ◽  
Cellulose Membrane ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Nutritional Factors ◽  
Obligate Parasite ◽  
Ribes Spp

Tissue cultures derived from the cortex of Pseudotsuga menziesii provided the nutrients required to support growth of Cronartium ribicola, a rust fungus. In nature, this obligate parasite is sustained by Pinus and Ribes spp. only. The pathogen was separated from its source of nutrients by a cellulose membrane. These experiments demonstrated that the nutritional factors required for growth of this rust are not host-specific and have small molecular size.

In vivo verification of Cronartium ribicola propagated on tissue cultures of Pinus monticola

1970 ◽  
Vol 48 (7) ◽  
pp. 1429-1430 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Infected Tissue ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Rust Infection ◽  
Western White Pine ◽  
Host Tissues

Inoculations of western white pine seedling stems with rust-infected tissue cultures produced one successful rust infection after 5 months. The infection was typical of this rust (Cronartium ribicola J. C. Fisch. ex Rabenh.) and the presence of haustoria was confirmed. Infected cortex tissue from this seedling was used to reestablish the isolate on host tissues grown in vitro.

Ultrastructure of tissue cultures of Pinus monticola infected by Cronartium ribicola. II. Penetration and post-penetration

1975 ◽  
Vol 5 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Jane Robb ◽  
A.E. Harvey ◽  
Michael Shaw
Keyword(s):  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola

Growth of Cronartium ribicola in the absence of physical contact with its host

1970 ◽  
Vol 48 (1) ◽  
pp. 71-73 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Host Tissue ◽  
Physical Contact ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Western White Pine ◽  
Agar Media ◽  
Healthy Host

Rust mycelia from tissue cultures infected with Cronartium ribicola J. C. Fisch. ex Rabenh. were grown on cellophane membranes in the absence of physical contact with host tissue. These membranes were in contact with agar media containing imbedded, actively growing western white pine (Pinus monticola Dougl.) tissue cultures. Identities of these cultures were verified by reestablishing infections in healthy host tissue cultures.

Inoculation of western white pine tissue cultures with basidiospores of Cronartium ribicola

1970 ◽  
Vol 48 (7) ◽  
pp. 1309-1311 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Western White Pine ◽  
Direct Inoculation

Rust infections were established in healthy, undifferentiated tissue cultures of Pinus monticola Dougl. by direct inoculation with germinating basidiospores of Cronartium ribicola J. C. Fisch. ex Rabenh. Infections were verified by confirming the presence of typical rust haustoria and extensive intracellular ramification by the mononucleate rust mycelium.

Polysaccharides of plant tissue cultures. III. Enzymatic hydrolysis of industrial wastes of the biomass of a ginseng tissue culture

1988 ◽  
Vol 24 (4) ◽  
pp. 415-421
Author(s):  
E. P. Kukhta ◽  
I. V. Aleksandrova ◽  
S. P. Afanas'ev ◽  
M. A. Lyal'chenko
Keyword(s):  
Tissue Culture ◽  
Enzymatic Hydrolysis ◽  
Plant Tissue ◽  
Industrial Wastes ◽  
Tissue Cultures ◽  
Plant Tissue Cultures ◽  
Hydrolysis Of
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