Ultrastructure of hyphal walls and septa of Cronartium ribicola on tissue cultures of Pinus monticola

1973 ◽  
Vol 51 (12) ◽  
pp. 2301-2305 ◽  
Author(s):  
Jane Robb ◽  
A. E. Harvey ◽  
Michael Shaw
Keyword(s):  
Electron Microscopy ◽  
Rust Fungus ◽  
Callus Cultures ◽  
Fungal Mycelium ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
Host Parasite ◽  
Lateral Walls

Colonies of the rust fungus Cronartium ribicola J. C. Fischer were grown axenically and then transferred so that they lay immediately next to callus cultures of the pine host (Pinus monticola Dougl.). Twelve days after the establishment of this association samples of the fungal mycelium taken 2 mm away from the pine callus and at the host–parasite interface were fixed for electron microscopy. Differences were found in the appearance and thickness of the lateral walls and extracellular coatings depending on the proximity of the fungus to the host–parasite interface. Septa were intermediate in structure between the Ascomycete and dolipore types. The origin of the extracellular sheath is discussed.

The relative susceptibility of needle- and stem-derived white pine tissue cultures to artificial inoculation with mycelium of Cronartium ribicola

1969 ◽  
Vol 47 (11) ◽  
pp. 1789-1790 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Rust Fungus ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Relative Susceptibility ◽  
Blister Rust ◽  
Leaf Mesophyll ◽  
Leaf Tissues ◽  
Artificial Inoculations

Tissue cultures of Pinus monticola Dougl. derived from stem cortex and leaf tissues were found susceptible to artificial inoculations with mycelium from the blister rust fungus (Cronartium ribicola Fisch. ex Rabenh.). Tissue cultures from leaf mesophyll grew slower and were colonized more rapidly by this fungus than those derived from stem cortex.

Inoculation of a nonhost tissue culture with Cronartium ribicola

1971 ◽  
Vol 49 (6) ◽  
pp. 881-882 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Tissue Culture ◽  
Pseudotsuga Menziesii ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola

Cultured tissues from Pseudotsuga menziesii (Mirb.) Franco were successfully inoculated with Cronartium ribicola J. C. Fisch. ex Rabenh. Typical intracellular haustoria were formed 60 days after inoculation. Invaded nonhost tissues were necrotic within 90 days, and dead within 120 days. Tissue cultures of either Pinus monticola Dougl. or P. menziesii had an attractant effect on nearby rust mycelium.

Histopathology and Cytology of Cronartium ribicola in Tissue Cultures of Pinus monticola

1971 ◽  
Vol 61 (7) ◽  
pp. 773 ◽  
Author(s):  
A. E. Harvey
Keyword(s):  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola

THE PHYSIOLOGY OF HOST–PARASITE RELATIONS: XVIII. DISTRIBUTION OF TRITIUM-LABELLED CYTIDINE, URIDINE, AND LEUCINE IN WHEAT LEAVES INFECTED WITH THE STEM RUST FUNGUS

1967 ◽  
Vol 45 (5) ◽  
pp. 555-563 ◽  
Author(s):  
P. K. Bhattacharya ◽  
Michael Shaw
Keyword(s):  
Stem Rust ◽  
Rust Fungus ◽  
Host Cells ◽  
Fungal Mycelium ◽  
Puccinia Graminis ◽  
Mesophyll Cells ◽  
Wheat Leaves ◽  
Host Parasite

Wheat leaves were detached 6 days after inoculation with the stem rust fungus (Puccinia graminis var. tritici Erikss. and Henn.) and fed with tritiated leucine, cytidine, uridine, or thymidine. Mesophyll cells in infected zones incorporated more leucine into protein and more cytidine and uridine into RNA than did cells in adjacent uninfected tissue. Leucine, cytidine, and uridine were also heavily incorporated by fungal mycelium and developing uredospores. Grain counts over host nuclei in the infected zone were two to three-fold of those over nuclei in adjacent uninfected zones. There was no detectable incorporation of thymidinemethyl-3H into either the fungus or the host cells. The results are discussed.

Ultrastructure of tissue cultures of Pinus monticola infected by Cronartium ribicola. I. Prepenetration host changes

1975 ◽  
Vol 5 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Jane Robb ◽  
A.E. Harvey ◽  
Michael Shaw
Keyword(s):  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola

Resistance to Cronartium ribicola in Pinus monticola: reduced needle-spot frequency

1980 ◽  
Vol 58 (5) ◽  
pp. 574-577 ◽  
Author(s):  
R. J. Hoff ◽  
G. I. McDonald
Keyword(s):  
Mating System ◽  
Rust Fungus ◽  
Low Frequency ◽  
Resistance Factor ◽  
Cronartium Ribicola ◽  
Pinus Monticola ◽  
Horizontal Resistance ◽  
White Pine ◽  
Blister Rust ◽  
Western White Pine

Low frequency of needle spots caused by the blister rust fungus (Cronartium ribicola J. C. Fisch. ex Rabenh.) in western white pine (Pinus monticola Dougl.) appears to be an expression of a horizontal resistance factor in secondary needles. Heritability averaged 37% for two sets of a 4 tester × 10 candidate mating system. We discuss the implications of these results with respect to developing varieties of western white pine resistant to blister rust.

Production of the nutritional requirements for growth of Cronartium ribicola by a nonhost species

1971 ◽  
Vol 49 (8) ◽  
pp. 1517-1519 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Pseudotsuga Menziesii ◽  
Rust Fungus ◽  
Molecular Size ◽  
Nutritional Requirements ◽  
Cellulose Membrane ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Nutritional Factors ◽  
Obligate Parasite ◽  
Ribes Spp

Tissue cultures derived from the cortex of Pseudotsuga menziesii provided the nutrients required to support growth of Cronartium ribicola, a rust fungus. In nature, this obligate parasite is sustained by Pinus and Ribes spp. only. The pathogen was separated from its source of nutrients by a cellulose membrane. These experiments demonstrated that the nutritional factors required for growth of this rust are not host-specific and have small molecular size.

In vivo verification of Cronartium ribicola propagated on tissue cultures of Pinus monticola

1970 ◽  
Vol 48 (7) ◽  
pp. 1429-1430 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Infected Tissue ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Rust Infection ◽  
Western White Pine ◽  
Host Tissues

Inoculations of western white pine seedling stems with rust-infected tissue cultures produced one successful rust infection after 5 months. The infection was typical of this rust (Cronartium ribicola J. C. Fisch. ex Rabenh.) and the presence of haustoria was confirmed. Infected cortex tissue from this seedling was used to reestablish the isolate on host tissues grown in vitro.

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