Biochemical differentiation in flax tissue culture. Phenolic compounds

1973 ◽  
Vol 51 (4) ◽  
pp. 820-823 ◽  
Author(s):  
Sally Liau ◽  
R. K. Ibrahim
Keyword(s):  
Tissue Culture ◽  
Phenolic Compounds ◽  
White Light ◽  
Culture Medium ◽  
Callus Tissue ◽  
Agar Medium ◽  
Coconut Water ◽  
Growth Substances ◽  
Biochemical Differentiation

Flax cotyledonary tissue loses its biosynthetic potential for flavonoid and sinapoyl compounds when grown on agar medium containing sucrose, NAA, kinetin, and coconut water. The pattern of phenolic compounds of the callus tissue, however, exhibits a marked differentiation which is related to the levels of growth substances or phenolic precursors added to the culture medium. The induced formation of a C-glycoflavone (iso-orientin) and an anthocyanin (cyanidin-3,5-diglucoside) under high levels of kinetin and white light was demonstrated.

scholarly journals Pengaruh Komposisi Media dalam Perbanyakan Protocorm Like Bodies, Pertumbuhan Planlet, dan Aklimatisasi Phalaenopsis amabilis

2015 ◽  
Vol 4 (3) ◽  
pp. 131 ◽  
Author(s):  
Erick Raynalta ◽  
Dewi Sukma
Keyword(s):  
Tissue Culture ◽  
Survival Rate ◽  
Culture Medium ◽  
Culture Media ◽  
Coconut Water ◽  
Basic Medium ◽  
Water Medium ◽  
Media Composition ◽  
Fresh Weight ◽  
Tissue Culture Media

<p>ABSTRACT</p><p>Phalaenopsis  amabilis  orchid  is one   of the most  popular  species  of orchids and  was inaugurated as one of the  national  flower ' Puspa  Pesona'. The purpose  of this study  was to determine the effects of   various  tissue culture   media composition on  Protocorm Like Bodies (PLBs)  multiplication,  growth  of plantlets,  and  acclimatization  of  Phalaenopsis  amabilis. Basic medium  used was  a half strengh of  Murashige and  Skoog (1/2MS)  and  Hyponex with additional  coconut  water,  benzyladeninepurine,  and  chitosan.  In  PLBs multiplication experiments ,   ½  MS  +  15%  coconut  water  medium produces  the  highest   percentage  of survival  rate.  ½  MS  +  15%  coconut water  medium  and  Hyponex  2  g  L-1+  15%  coconut water  +  2.5 ppm chitosan  medium  gave  the best  effect on  the fresh   weight  of  plantlets. The composition of  the culture medium   significantly affect   the fresh   weight and length of   root   on 8  WAP  (week after planting)   in the   acclimatization stage.</p><p>Key words: benzyladeninepurine, chitosan, coconut water, Hyponex,  tissue culture</p><p>ABSTRAK</p><p>Phalaenopsis amabilis  yang dikenal dengan  nama anggrek bulan merupakan salah satu jenisanggrek  yang  paling  populer  dan  dinobatkan  sebagai  salah satu  bunga  nasional  dengan  sebutan ‘Puspa Pesona’.  Tujuan penelitian ini untuk  mengetahui pengaruh berbagai komposisi media kultur jaringan dalam perbanyakan  Protocorm Like Bodies  (PLBs),  pertumbuhan  planlet, dan aklimatisasi Phalaenopsis amabilis. Media dasar yang digunakan adalah setengah konsentrasi media  Murashige dan  Skoog  (1/2MS)  dan  Hyponex dengan  tambahan  air  kelapa,  benziladeninpurin  (BAP),  dan kitosan. Pada percobaan perbanyakan PLBs, media ½ MS + 15% air kelapa menghasilkan persentase hidup tertinggi.  Media ½ MS + 15% air kelapa dan Hyponex  2 g L-1+ 15% AK + 2.5 ppm  kitosanmemberikan  respon  terbaik  terhadap  bobot planlet.  Komposisi  media  kultur  berpengaruh  nyata terhadap bobot segar dan panjang akar pada 8-MST (minggu setelah tanam) dalam tahap aklimatisasi.</p><p>Kata kunci: benzylaminopurin, kitosan, air kelapa, Hyponex, kultur jaringan</p>

scholarly journals Identity of the Fungal Symbiont of Sirex Noctilio

1969 ◽  
Vol 22 (4) ◽  
pp. 905 ◽  
Author(s):  
I PC Gaut
Keyword(s):  
Tissue Culture ◽  
Pinus Radiata ◽  
Gel Electrophoresis ◽  
Culture Medium ◽  
Callus Tissue ◽  
Starch Gel Electrophoresis ◽  
Polystyrene Film ◽  
Fungal Symbiont ◽  
Sirex Noctilio ◽  
Starch Gel

The fungus associated with S. noctilio F. in Australia is identified as Amyloster-eum areolatum (Fr.) Boidin. By using a polystyrene film to prevent the breakup of the chains of arthrospores during staining, it was possible to demonstrate that the spores are homokaryotic though usually multinucleate. Viable homokaryons were established from arthrospores by using a tissue culture medium incorporating growing callus tissue of Pinus radiata. Homokaryons and heterokaryons of the S. noctilio fungus were opposed in all possible combinations against homokaryons and heterokaryons of A. chailleti'i (Pers. ex Fr.) Boidin, A.laevigatum (Fr.) Boidin, and A. areolatum, the only known species of Amylostereum. The S. noctilio fungus was interfertile only with A. areolatum, as judged by production of anastomoses, clamped mycelium, and fertile fruit bodies. Starch-gel electrophoresis showed that the soluble proteins of the S. noctilio fungus corresponded more closely with those of A. areolatum than with those of A. chailletii.

scholarly journals ULASAN Masalah Pencoklatan pada Kultur Jaringan

2016 ◽  
Vol 4 (2) ◽  
pp. 83 ◽  
Author(s):  
Sri Hutami
Keyword(s):  
Tissue Culture ◽  
Phenolic Compounds ◽  
Redox Potential ◽  
Plant Species ◽  
Culture Medium ◽  
Substrate Availability ◽  
Plant Parts ◽  
Tropical Plant ◽  
High Concentrations

<p>Several<br />tropical plant species contain high concentrations of phenolic<br />compounds, which become oxidised when their cells<br />are wounded or when the plant parts become senescences.<br />In tissue culture, the phenolic compounds usually leach into<br />the medium from the cut surfaces of explants. The phenolic<br />compounds caused the culture medium turns to dark brown<br />in colour due to oxidation. This is detrimental to the culture,<br />because it causes the isolated tissue fails to grow. The<br />browning of tissue culture and the medium can often be<br />prevented by one of the several different approaches, such<br />as by removing the phenolic compounds produced, modifying<br />the redox potential, inactivating phenolase enzymes,<br />reducing phenolase activity and substrate availability, as well<br />as pre-treatments by soaking and preconditioning on a basal<br />medium.</p>

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

ON TETHELIN AS A TISSUE CULTURE MEDIUM

1930 ◽  
Vol 1 (9) ◽  
pp. 289-290
Author(s):  
K. C. Richardson ◽  
E. S. Horning
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Tissue Culture Medium

scholarly journals In Vitro Production of Erythropoietin by Kidneys Perfused With a Serum-free Solution

Blood ◽  
1974 ◽  
Vol 44 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Allan J. Erslev
Keyword(s):  
Tissue Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Kidney Tissue ◽  
In Vitro Production ◽  
In Vitro Perfusion ◽  
Serum Free ◽  
Enzymatic Activation ◽  
Vitro Production

Abstract Normal rabbits exposed to 0.4 atmospheric pressure for 3 hr will generate about 40-60 U of erythropoietin during a subsequent 3-hr period. If the kidneys were removed from 3-hr hypoxic animals, washed carefully, and perfused for 3 hr by recirculation with a serum-tissue culture mixture, each kidney generated about 14 U of erythropoietin in vitro. Perfusion of normal kidneys did not result in the production of erythropoietin, and only small amounts were generated if the perfusate contained Puromycin. Three-hour hypoxic kidneys perfused for 3 hr with a serum-free tissue culture medium were found to generate about 8 U of erythropoietin per kidney and similar kidneys perfused with saline about 1 U. These results indicate that erythropoietin is synthesized by kidney tissue and not produced by enzymatic activation of a plasma substrate.

scholarly journals THE ANTIGENIC POTENCY OF EPIDEMIC INFLUENZA VIRUS FOLLOWING INACTIVATION BY ULTRAVIOLET RADIATION

1940 ◽  
Vol 72 (6) ◽  
pp. 729-745 ◽  
Author(s):  
Jonas E. Salk ◽  
G. I. Lavin ◽  
Thomas Francis
Keyword(s):  
Tissue Culture ◽  
Influenza Virus ◽  
Ultraviolet Radiation ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Intraperitoneal Route ◽  
Active Virus ◽  
High Concentrations

A study of the antigenic potency of influenza virus inactivated by ultraviolet radiation has been made. Virus so inactivated is still capable of functioning as an immunizing agent when given to mice by the intraperitoneal route. In high concentrations inactivated virus appears to be nearly as effective as active virus but when quantitative comparisons of the immunity induced by different dilutions are made, it is seen that a hundredfold loss in immunizing capacity occurs during inactivation. Virus in suspensions prepared from the lungs of infected mice is inactivated more rapidly than virus in tissue culture medium. A standard for the comparison of vaccines of epidemic influenza virus is proposed.

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