Evaluation of the pressure chamber technique for measurement of leaf water potential in cassava (Manihot species)

1978 ◽  
Vol 56 (14) ◽  
pp. 1638-1641 ◽  
Author(s):  
I. F. Ike ◽  
G. W. Thurtell ◽  
K. R. Stevenson
Keyword(s):  
Water Potential ◽  
Leaf Water Potential ◽  
Pressure Chamber ◽  
Leaf Water ◽  
Dew Point ◽  
Manihot Esculenta Crantz ◽  
Osmotic Potentials ◽  
Chamber Technique ◽  
Cassava Varieties

The pressure chamber technique was evaluated as a method for estimating leaf water potential in cassava (Manihot esculenta Crantz). Xylem pressure potentials (ψP) measured with the pressure chamber were compared with leaf water potential (ψL) obtained for the same leaf with the in situ dew-point hygrometer.In both cassava varieties studied, ψL and ψP were linearly related (r2 = 0.87 and 0.98 for CMC9 and CMC40 respectively). The length of petiole exposed outside the chamber affects the relation between ψL and ψP and should be kept at between 1 and 3 cm for better agreement. In CMC40, ψP was consistently lower (drier) than ψL by about 1.0 bar (1 bar = 100 kPa) in the entire range of water potential studied, but was not the case in CMC9. The reason for this difference is unclear but may be due to a filling of tissues other than xylem tissues (Boyer 1967) during the measurement of ψP in CMC40. Average xylem osmotic potentials (ψS) were low (−1.0 ± 0.2 bars and −1.0 ± 0.4 bars for CMC9 and CMC40 respectively). It is, therefore, unnecessary to correct for ψS when using the pressure chamber to estimate leaf water potentials in cassava.

The relationship between steady-state transpiration rates and leaf water potential in cassava (Manihot esculenta cv. Llanera)

1978 ◽  
Vol 56 (13) ◽  
pp. 1537-1539 ◽  
Author(s):  
I. F. Ike ◽  
G. W. Thurtell ◽  
K. R. Stevenson
Keyword(s):  
Steady State ◽  
Water Potential ◽  
Leaf Water Potential ◽  
Manihot Esculenta ◽  
Regression Line ◽  
Leaf Water ◽  
Dew Point ◽  
Manihot Esculenta Crantz ◽  
The Relationship

The relationship between leaf water potential (ψL) and transpiration rate (T) was investigated using indoor-grown cassava plants (Manihot esculenta Crantz cv. Llanera). Leaf water potentials were measured with in situ dew-point hygrometer and transpiration rates by gas exchange analysis technique.Regression analyses of the data showed that T was consistently linearly related to ψL (r2 = 0.94). This implies that the plant resistance to flow was constant and hence that an Ohm's Law analog is valid for the transpiration range studied. Extrapolated values of leaf water potential at zero transpiration were close to the osmotic potential of the nutrient solution. Calculated resistance values (slope of regression line for individual plants) varied between 2.90 and 3.05 bars dm2 h g−1 (1 bar = 100 kPa).

A Comparison of Thermocouple Psychrometer and Pressure Chamber Measurements of Leaf Water Potential in Peanuts

1988 ◽  
Vol 24 (3) ◽  
pp. 355-359 ◽  
Author(s):  
G. C. Wright ◽  
A. Rahmianna ◽  
P. M. Hatfield
Keyword(s):  
Water Stress ◽  
Water Potential ◽  
Leaf Water Potential ◽  
Comparative Studies ◽  
Pressure Chamber ◽  
Leaf Water ◽  
Thermocouple Psychrometer ◽  
Absolute Measurements ◽  
Chamber Measurements ◽  
Chamber Technique

SUMMARYThe pressure chamber method for measuring leaf water potential in peanuts was compared with the thermocouple psychrometer technique. The pressure chamber over-estimated leaf water potential by an average of 0.4 MPa over the range −0.5 to −5.0 MPa. It is suggested that the pressure chamber technique may be appropriate in comparative studies of peanut water stress. However, where absolute measurements are required, as in the calculation of leaf turgor potential, either a correction factor should be applied or, preferably, the thermocouple psychrometer technique used. If no account is taken of the discrepancy between techniques, erroneously large negative turgor potentials will result.

scholarly journals Hydraulic Press Measurements of Leaf Water Potential in Groundnuts

1983 ◽  
Vol 19 (4) ◽  
pp. 287-291 ◽  
Author(s):  
G. Rajendrudu ◽  
M. Singh ◽  
J. H. Williams
Keyword(s):  
Regression Analysis ◽  
Water Potential ◽  
Leaf Water Potential ◽  
Error Variance ◽  
Hydraulic Press ◽  
Pressure Chamber ◽  
Leaf Water ◽  
Dew Point ◽  
Functional Relations ◽  
The Press

SUMMARYThe hydraulic press was compared with the dew point psychrometer and the pressure chamber methods for measuring leaf water potential (Ψ) in groundnuts (Arachis hypogaea L.). For measurements on the same leaf, regression analysis revealed that the slopes did not differ significantly from unity. An analysis of functional relations between measurements made by the press and the dew point psychrometer or the press and the pressure chamber showed that the error variance of the press was similar to those of the two other methods. Therefore, we conclude that for groundnuts the performance of the press, the dew point psychrometer and the pressure chamber are similar.

Evaluation of the hydraulic press for measurement of leaf water potentials in cassava

1983 ◽  
Vol 101 (2) ◽  
pp. 407-410 ◽  
Author(s):  
J. A. Palta
Keyword(s):  
Water Potential ◽  
Leaf Water Potential ◽  
Hydraulic Press ◽  
Pressure Chamber ◽  
Leaf Water ◽  
Moisture Loss ◽  
Total Leaf Area ◽  
Water Potentials ◽  
Wide Range ◽  
Poor Relationship

SUMMARYIn the application of the Scholander pressure chamber technique to cassava water relations studies, the leaf water potential measured on central lobules was initially compared with that measured on entire leaves (including petiole). Measurements made using both a Campbell-Brewster hydraulic press and a pressure chamber of the leaf water potential in six different cassava clones were also compared. Although the central lobules showed a greater sensitivity to moisture loss after sampling than entire leaves, their leaf water potential was in close agreement with those measured on the entire leaves (r3 = 0·96). Therefore, for routine and field estimates in cassava, measurements made on the central lobules may be used to avoid the large reduction in total leaf area. The Campbell-Brewster hydraulic press satisfactorily estimated leaf water potential in M.Col. 1684 clone, which had the longest and narrowest lobules, but in other clones the leaf water potential was overestimated at high leaf potential (> -12·5) and underestimated at low water potentials (< -12·5). Over a wide range of leaf water potentials, a poor relationship between leaf water potentials estimated with hydraulic press and with the pressure chamber was observed for cassava because press estimates are influenced by lobule length and lobule width.

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