Ultrastructural localization of calcium in the organic acid secreting trichomes of chickpea (Cicer arietinum)

1992 ◽  
Vol 70 (12) ◽  
pp. 2319-2325 ◽  
Author(s):  
Mark D. Lazzaro ◽  
William W. Thomson
Keyword(s):  
Organic Acid ◽  
Cicer Arietinum ◽  
Cell Walls ◽  
Ultrastructural Localization ◽  
Secretory Vesicles ◽  
X Ray ◽  
Cicer Arietinum L ◽  
Calcium Deposits ◽  
Acid Secreting ◽  
Calcium Secretion

In the organic acid secreting trichomes of chickpea (Cicer arietinum L.), calcium is localized in the stalk and head cells using pyroantimonate and X-ray microanalysis. Light calcium deposits are present in the endoplasmic reticulum, mitochondria, and cytoplasm of the stalk and head cells and in the cell walls of the stalk cells. Dense calcium deposits are present in the vacuoles of stalk and head cells and within the plasmodesmata between stalk cells. In the head cells, heavy calcium deposits are present in small, secretory vesicles that fuse with the plasma membrane. In addition, calcium deposits are localized in the cell wall space around the head cells, in the secretion chamber, and in collected secretions. These observations suggest that secretion from the head cells occurs predominantly through an exocytotic, vesicular pathway. We conclude that once secreted, calcium diffuses through the walls to the collecting chamber and subsequently through the cuticular pores into the secretion droplet. Key words: calcium secretion, Cicer arietinum, pyroantimonate, secretory vesicles, trichome.

ENDOCYTOSIS OF LANTHANUM NITRATE IN THE ORGANIC ACID-SECRETING TRICHOMES OF CHICKPEA (CICER ARIETINUM L.)

1992 ◽  
Vol 79 (10) ◽  
pp. 1113-1118 ◽  
Author(s):  
Mark D. Lazzaro ◽  
William W. Thomson
Keyword(s):  
Organic Acid ◽  
Cicer Arietinum ◽  
Lanthanum Nitrate ◽  
Cicer Arietinum L ◽  
Acid Secreting

Ultrastructure of organic acid secreting trichomes of chickpea (Cicer arietinum)

1989 ◽  
Vol 67 (9) ◽  
pp. 2669-2677 ◽  
Author(s):  
Mark D. Lazzaro ◽  
William W. Thomson
Keyword(s):  
Endoplasmic Reticulum ◽  
Granular Material ◽  
Organic Acid ◽  
Cicer Arietinum ◽  
Basal Cell ◽  
Fixed Carbon ◽  
Mesophyll Cells ◽  
Dense Cytoplasm ◽  
Acid Secreting ◽  
Organic Acid Secretion

The acid-secreting trichomes of chickpea (Cicer arietinum L.) were composed of 18 cells, including 1 basal cell, 3 elongate stalk cells, and 14 head cells. A subcuticular secretion chamber with cuticular pores was present above the head cells at the trichome tip. The basal and stalk cells had large central vacuoles, endoplasmic reticulum, mitochondria, and small vacuoles. In the stalk cells, these small vacuoles were aligned along microtubles extending from the bottom to the top of the cells. Head cells had more dense cytoplasm than stalk cells and also had numerous mitochondria and small vacuoles. A labyrinth of tubules and vesicles at the edges of the head cells contained granular material similar to that observed in the extraplasmic space of the head cell and in the secretion chamber. In older head cells, the tubules were thinner and lacked granular material, the cells contained sequestering membranes and vacuoles, and calcium oxalate crystals were observed in the extraplasmic space. Plasmodesmata were not observed between the basal cell and the surrounding mesophyll cells, although numerous plasmodesmata with associated desmotubules and endoplasmic reticulum connected the trichome cells. Chloroplasts were not observed in the head or stalk cells, whereas the basal cell had small chloroplasts with reduced thylakoid networks and the mesophyll cells had large chloroplasts with well-developed thylakoids that may provide the fixed carbon for organic-acid secretion.

scholarly journals Pectin Depolymerase Activities Associated with Cell Walls from Cicer arietinum L. Epicotyl

1997 ◽  
Vol 38 (11) ◽  
pp. 1259-1263 ◽  
Author(s):  
J. M. Sevillano ◽  
I. Zarra ◽  
J. L. Acebes
Keyword(s):  
Cicer Arietinum ◽  
Cell Walls ◽  
Cicer Arietinum L

The effect of varied fertilization on the yield and chemical composition of chickpea seed (Cicer arietinum L.)

2019 ◽  
Vol 23 (1) ◽  
Author(s):  
Volodymyr Lykhochvor ◽  
◽  
Volodymyr Pushchak ◽  
Keyword(s):  
Chemical Composition ◽  
Cicer Arietinum ◽  
Cicer Arietinum L

DIFERENCIACIÓN DE ACCESIONES DE GARBANZO MEDIANTE CARACTERES MORFOLÓGICOS Y MOLECULARES

2018 ◽  
Vol 41 (4) ◽  
pp. 469-476
Author(s):  
Ernestina Valadez-Moctezuma ◽  
Anselmo de J. Cabrera-Hidalgo
Keyword(s):  
Cicer Arietinum ◽  
Cicer Arietinum L

El garbanzo (Cicer arietinum L.) es una de las principales leguminosas de grano cultivadas en el mundo. México es de los diez principales productores a nivel mundial con una producción total de 171 mil toneladas de grano. Este cultivo presenta alto nivel de autogamia y de monotonía genética, lo que dificulta la diferenciación de genotipos élite. En este estudio preliminar se evaluó la variabilidad de 57 genotipos de diferentes áreas geográficas mediante caracteres morfológicos y marcadores moleculares tipo RAPD e ISSR. El análisis de correspondencia múltiple mostró que los mayores valores discriminantes fueron el color de la flor azul y semillas grandes y de forma redondeada, pero el agrupamiento respectivo no diferenció a las accesiones, incluyendo las especies silvestres; sin embargo, el análisis UPGMA logró una mejor separación. Los marcadores RAPD aun cuando generaron perfiles de ADN, no fueron informativos, mientras que los ISSR diferenciaron a las 57 accesiones de C. arietinum utilizadas y a la especie silvestre C. reticulatum, lo que los hace buenos candidatos para caracterizar este cultivo. Este estudio sirvió como base para desarrollar otro sistema de marcadores moleculares más eficiente en esta especie.

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