Genetic analysis of pyriform microconidium production in mating population B of Gibberella fujikuroi

1994 ◽  
Vol 72 (8) ◽  
pp. 1209-1210 ◽  
Author(s):  
Fa-Jun Chen
Keyword(s):  
Genetic Analysis ◽  
Key Words ◽  
Single Gene ◽  
Gibberella Fujikuroi ◽  
Fusarium Subglutinans ◽  
Mendelian Gene ◽  
Mating Population

Isolates of Fusarium subglutinans that differ in their ability to produce globose or pyriform microconidia were sexually compatible members of mating population B of Gibberella fujikuroi. Single ascospore isolates from these matings segregated 1:1 for the production of globose or pyriform microconidia, suggesting that a single Mendelian gene controls production of globose or pyriform microconidia. The validity of Fusarium anthophilum as an anamorph species is questioned. Key words: pyriform microconidium, single gene, Fusarium subglutinans, Fusarium anthophilum.

Gibberella fujikuroi mating population A and Fusarium subglutinans from teosinte species and maize from Mexico and Central America

2000 ◽  
Vol 104 (7) ◽  
pp. 865-872 ◽  
Author(s):  
A.E. Desjardins ◽  
R.D. Plattner ◽  
T.R. Gordon
Keyword(s):  
Central America ◽  
Gibberella Fujikuroi ◽  
Fusarium Subglutinans ◽  
Mating Population

scholarly journals Fusarium subglutinans f. sp.pini Represents a Distinct Mating Population in theGibberella fujikuroi Species Complex

1999 ◽  
Vol 65 (3) ◽  
pp. 1198-1201 ◽  
Author(s):  
H. Britz ◽  
T. A. Coutinho ◽  
M. J. Wingfield ◽  
W. F. O. Marasas ◽  
T. R. Gordon ◽  
...  
Keyword(s):  
South Africa ◽  
Species Complex ◽  
Biological Species ◽  
Gibberella Fujikuroi ◽  
Pitch Canker ◽  
Fusarium Subglutinans ◽  
Mating Population ◽  
Standard Tester ◽  
Distinct Taxon

ABSTRACT Fusarium strains in the Gibberella fujikuroi species complex cause diseases on a variety of economically important plants. One of these diseases, pitch canker of Pinus spp., is caused by strains identified asFusarium subglutinans f. sp. pini. Fertile crosses were detected between F. subglutinans f. sp.pini strains from South Africa, California, and Florida.F. subglutinans f. sp. pini strains were not cross-fertile with the standard tester strains of six of the seven other mating populations of G. fujikuroi. Sporadic perithecia with ascospores were obtained in two crosses with the mating population B tester strains. These perithecia were homothallic, and the ascospores derived from these perithecia were vegetatively compatible with the mating population B tester strain parent. We concluded that fertile F. subglutinans f. sp. pini isolates represent a new mating population (mating population H) of G. fujikuroi and that they belong to a unique biological species in a distinct taxon.

scholarly journals A Genetic Map of Gibberella fujikuroi Mating Population A (Fusarium moniliforme)

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 175-189 ◽  
Author(s):  
Jin-rong Xu ◽  
John F Leslie
Keyword(s):  
Fusarium Moniliforme ◽  
Fragment Length Polymorphism ◽  
Fumonisin B1 ◽  
Gibberella Fujikuroi ◽  
Female Sterility ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Mating Population ◽  
Spore Killer ◽  
Chiasma Interference

Abstract We constructed a recombination-based map of the fungal plant pathogen Gibberella fujikuroi mating population A (asexual stage Fusarium moniliforme). The map is based on the segregation of 142 restriction fragment length polymorphism (RFLP) markers, two auxotrophic genes (arg1, nic1), mating type (matA+ / matA−), female sterility (ste1), spore-killer (Sk), and a gene governing the production of the mycotoxin fumonisin B1 (fum1) among 121 random ascospore progeny from a single cross. We identified 12 linkage groups corresponding to the 12 chromosome-sized DNAs previously observed in contour-clamped homogeneous electric field (CHEF) gels. Linkage groups and chromosomes were correlated via Southern blots between appropriate RFLP markers and the CHEF gels. Eleven of the 12 chromosomes are meiotically stable, but the 12th (and smallest) is subject to deletions in 3% (4/121) of the progeny. Positive chiasma interference occurred on five of the 12 chromosomes, and nine of the 12 chromosomes averaged more than one crossover per chromosome. The average kb/cM ratio in this cross is ~32.

scholarly journals GENETIC ANALYSIS OF FAMILIES HAVING AUTOSOMAL RECESSIVE INTELLECTUAL DISABILITY

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Jamshed Khan ◽  
Muhammad Junaid ◽  
Shahab Uddin ◽  
Khalida Moeed ◽  
Usman Ullah ◽  
...  
Keyword(s):  
Intellectual Disability ◽  
Genetic Analysis ◽  
Single Gene ◽  
Cross Sectional Study ◽  
Molecular Diagnostic ◽  
Developmental Defect ◽  
Chromosomal Disorders ◽  
Cross Sectional ◽  
Pathogenic Variants ◽  
Development Delay

Background: Intellectual disability (ID) is a neuro-developmental defect that is manifested by development delay and learning disability. Such defects may be caused due to chromosomal disorders (trisomy 18 or Down syndrome) or single gene mutation. Its worldwide prevalence is estimated to be 1-3%. The genetic etiology of non-syndromic ID is poorly understood. To date, more than 100 loci have been reported to be associated with non-syndromic ID. The objective of this study was to identify the causative genes for three Materials & Methods: This cross-sectional study was conducted in the Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan from March 2014 to August 2015. The inclusion criteria set for the families was consanguineous relation and more than two patients per family (including cousins). All the patients were tested individually in friendly atmosphere using IQ test to scale the ID on the basis of performance. Thereafter, blood samples were taken by aseptic method and DNA was extracted for the purpose of doing genetic analysis. In genetic analysis, exome sequencing was performed to find the pathogenic variants. Subsequently. Sanger sequencing was also done to see the segregation of pathogenic variants. Results: Genetic analysis found mutation in AP4B1 in Family 1, in WDR62 in Family 2, while Family 3 was unremarkable. Conclusion: The study involved genetic analysis of three consanguineous families and found mutation in AP4B1 in Family 1, in WDR62 in Family 2, while Family 3 was unremarkable. The present research will help in devising molecular diagnostic technics for pre-marital and pre-conception testing.

scholarly journals Collapsing-based and kernel-based single-gene analyses applied to Genetic Analysis Workshop 17 mini-exome data

2011 ◽  
Vol 5 (S9) ◽  
Author(s):  
Lun Li ◽  
Wei Zheng ◽  
Joon Sang Lee ◽  
Xianghua Zhang ◽  
John Ferguson ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Genetic Analysis Workshop ◽  
Single Gene ◽  
Exome Data

Incidence of Fusarium spp. and Levels of Fumonisin B1 in Maize in Western Kenya

1999 ◽  
Vol 65 (1) ◽  
pp. 41-44 ◽  
Author(s):  
C. J. Kedera ◽  
R. D. Plattner ◽  
A. E. Desjardins
Keyword(s):  
Poor Quality ◽  
Selective Medium ◽  
Gibberella Fujikuroi ◽  
Human Consumption ◽  
Ear Rot ◽  
Western Kenya ◽  
Maize Kernel ◽  
Smallholder Farm ◽  
High Incidence ◽  
Mating Population

ABSTRACT Maize kernel samples were collected in 1996 from smallholder farm storages in the districts of Bomet, Bungoma, Kakamega, Kericho, Kisii, Nandi, Siaya, Trans Nzoia, and Vihiga in the tropical highlands of western Kenya. Two-thirds of the samples were good-quality maize, and one-third were poor-quality maize with a high incidence of visibly diseased kernels. One hundred fifty-three maize samples were assessed for Fusarium infection by culturing kernels on a selective medium. The isolates obtained were identified to the species level based on morphology and on formation of the sexual stage inGibberella fujikuroi mating population tests.Fusarium moniliforme (G. fujikuroi mating population A) was isolated most frequently, but F. subglutinans (G. fujikuroi mating population E),F. graminearum, F. oxysporum, F. solani, and other Fusarium species were also isolated. The high incidence of kernel infection with the fumonisin-producing species F. moniliforme indicated a potential for fumonisin contamination of Kenyan maize. However, analysis of 197 maize kernel samples by high-performance liquid chromatography found little fumonisin B1 in most of the samples. Forty-seven percent of the samples contained fumonisin B1 at levels above the detection limit (100 ng/g), but only 5% were above 1,000 ng/g, a proposed level of concern for human consumption. The four most-contaminated samples, with fumonisin B1 levels ranging from 3,600 to 11,600 ng/g, were from poor-quality maize collected in the Kisii district. Many samples with a high incidence of visibly diseased kernels contained little or no fumonisin B1, despite the presence of F. moniliforme. This result may be attributable to the inability ofF. moniliforme isolates present in Kenyan maize to produce fumonisins, to the presence of other ear rot fungi, and/or to environmental conditions unfavorable for fumonisin production.

Preimplantation Diagnosis of Single Gene Disorders by Two-Step Oocyte Genetic Analysis Using First and Second Polar Body

1997 ◽  
Vol 62 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Yury Verlinsky ◽  
Svetlana Rechitsky ◽  
Jeanine Cieslak, Victor Ivakhnenko ◽  
George Wolf ◽  
Aaron Lifchez ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Single Gene ◽  
Polar Body ◽  
Preimplantation Diagnosis ◽  
Single Gene Disorders ◽  
Second Polar Body

Distribution and prevalence of Fusarium subglutinans in mango trees affected by malformation

1994 ◽  
Vol 72 (1) ◽  
pp. 7-9 ◽  
Author(s):  
R. C. Ploetz
Keyword(s):  
Key Words ◽  
Mangifera Indica ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Fusarium Subglutinans ◽  
Nit Mutants ◽  
Compatibility Group ◽  
Mango Malformation

Infection of malformed and nonmalformed mango (Mangifera indica) trees by Fusarium subglutinans was assessed in Florida. All malformed floral panicles, but only 50% of the nonmalformed panicles, were infected by the fungus. When within-panicle infection was evaluated, an average of 84.5 % of the small pedicel and peduncle tissue pieces from malformed panicles were infected. When malformed and nonmalformed panicles were both assessed, 68.3% of the tissues from malformed panicles, but only 11.7% from nonmalformed panicles, were infected. Slight infection (2.2%) was observed in branch tissue that supported malformed panicles, and the fungus was never isolated from branches that supported nonmalformed panicles. In nutritional complementation tests with nitrate nonutilizing (nit) mutants, 55 of 64 isolates of F. subglutinans from malformed mango panicles were in the same vegetative compatibility group. The data indicate that mango malformation in the study area was significantly correlated (P < 0.0001) with infection by genetically related populations of F. subglutinans. The far greater prevalence of F. subglutinans in malformed than in asymptomatic panicles suggests that malformation symptoms develop in floral tissues only after they are extensively colonized by this fungus. Key words: Fusarium subglutinans, mango malformation, vegetative compatibility.

Single gene mutants tolerant to NaCl in the fern Ceratopteris: Characterization and genetic analysis

Plant Science ◽  
1987 ◽  
Vol 52 (1-2) ◽  
pp. 49-55 ◽  
Author(s):  
Thomas R. Warne ◽  
Leslie G. Hickok
Keyword(s):  
Genetic Analysis ◽  
Single Gene
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