Fluorescent dye particles are good pollen analogs for hummingbird-pollinated Silene virginica (Caryophyllaceae)

1996 ◽  
Vol 74 (2) ◽  
pp. 189-193 ◽  
Author(s):  
Charles B. Fenster ◽  
Michele R. Dudash ◽  
Cynthia L. Hassler
Keyword(s):  
Fluorescent Dye ◽  
Good Pollen ◽  
Fluorescent Powder ◽  
Floral Visitation ◽  
Carry Over

We tested the utility of fluorescent dye particles as pollen analogs for hummingbird-pollinated Silene virginica (Caryophyllaceae) by comparing the movement of pollen and fluorescent dye across sequentially visited emasculated flowers. We found no differences in either the intercept or the slope of the regressions of the two particle types on floral visitation sequence. In addition, the presence of fluorescent powder on the stigmas of a flower was a good indicator of pollen transferred to that flower. Both pollen and dye particles were transported almost identical distances in the flower sequence. These data indicate that fluorescent powder is a good pollen analog for S. virginica. We compare our findings with previous studies examining the utility of fluorescent dye as a pollen analog. Keywords: pollen analog, fluorescent dye, pollen carry-over.

Contrasting fingerprints with Basic Yellow 40 – a methological algorithm

2019 ◽  
Vol 305 ◽  
pp. 60-65
Author(s):  
Katarzyna Drzewiecka ◽  
◽  
Ewa Rogoża ◽  
Keyword(s):  
Fluorescent Dye ◽  
Optimal Method ◽  
Physical Evidence ◽  
Fluorescent Powder ◽  
Special Cases ◽  
Plastic Surfaces ◽  
Macroscopic Examination

Basic Yellow 40 is a fluorescent dye used to contrast fingerprints revealed by the cyanoacrylate-based method. It is available as a yellow, crystalline, fluorescent powder. For visualization studies, Basic Yellow 40 is used as a solution, which is obtained by dissolving the powdered substance in alcohol or water. The solution is applied onto the surface by pouring, spraying, immersing or brushing. Excess of the solution can be rinsed off the surface with water or, in special cases, with alcohol. In forensic laboratories it is practiced to both rinse Basic Yellow 40 off the surface with water as well as to leave the surface to dry without rinsing. The aim of this study was to determine the algorithm for using Basic Yellow 40 for contrasting fingerprints deposited on plastic surfaces. The tests showed that the optimal method of treatment is to leave Basic Yellow 40 without rinsing and to conduct macroscopic examination of physical evidence, followed by rinsing excess dye with water if necessary.

3-Dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 164-165
Author(s):  
Thomas J. Deerinck ◽  
Maryann E. Martone ◽  
Varda Lev-Ram ◽  
David P. L. Green ◽  
Roger Y. Tsien ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Reactive Oxygen ◽  
Confocal Laser Scanning Microscope ◽  
Fluorescent Dye ◽  
Confocal Laser ◽  
3 Dimensional ◽  
Voltage Electron ◽  
Dimensional Distribution ◽  
Electron Microscopes ◽  
New Generation

The confocal laser scanning microscope has become a powerful tool in the study of the 3-dimensional distribution of proteins and specific nucleic acid sequences in cells and tissues. This is also proving to be true for a new generation of high contrast intermediate voltage electron microscopes (IVEM). Until recently, the number of labeling techniques that could be employed to allow examination of the same sample with both confocal and IVEM was rather limited. One method that can be used to take full advantage of these two technologies is fluorescence photooxidation. Specimens are labeled by a fluorescent dye and viewed with confocal microscopy followed by fluorescence photooxidation of diaminobenzidine (DAB). In this technique, a fluorescent dye is used to photooxidize DAB into an osmiophilic reaction product that can be subsequently visualized with the electron microscope. The precise reaction mechanism by which the photooxidation occurs is not known but evidence suggests that the radiationless transfer of energy from the excited-state dye molecule undergoing the phenomenon of intersystem crossing leads to the formation of reactive oxygen species such as singlet oxygen. It is this reactive oxygen that is likely crucial in the photooxidation of DAB.

Minimizing Carry-Over Effects After Treatment Failure and Maximizing Therapeutic Outcome

2014 ◽  
Vol 222 (3) ◽  
pp. 171-178 ◽  
Author(s):  
Mareile Hofmann ◽  
Nathalie Wrobel ◽  
Simon Kessner ◽  
Ulrike Bingel
Keyword(s):  
Treatment Failure ◽  
Human Subjects ◽  
Subsequent Treatment ◽  
Clinical Samples ◽  
Administration Route ◽  
Healthy Human ◽  
Negative Experiences ◽  
Analgesic Treatment ◽  
Balanced Design ◽  
Carry Over

According to experimental and clinical evidence, the experiences of previous treatments are carried over to different therapeutic approaches and impair the outcome of subsequent treatments. In this behavioral pilot study we used a change in administration route to investigate whether the effect of prior treatment experience on a subsequent treatment depends on the similarity of both treatments. We experimentally induced positive or negative experiences with a topical analgesic treatment in two groups of healthy human subjects. Subsequently, we compared responses to a second, unrelated and systemic analgesic treatment between both the positive and negative group. We found that there was no difference in the analgesic response to the second treatment between the two groups. Our data indicate that a change in administration route might reduce the influence of treatment history and therefore be a way to reduce negative carry-over effects after treatment failure. Future studies will have to validate these findings in a fully balanced design including larger, clinical samples.

Does CBT for Psychosis Have an Impact on Delusions by Improving Reasoning Biases and Negative Self-Schemas?

2018 ◽  
Vol 226 (3) ◽  
pp. 152-163 ◽  
Author(s):  
Stephanie Mehl ◽  
Björn Schlier ◽  
Tania M. Lincoln
Keyword(s):  
Behavioral Therapy ◽  
Theoretical Models ◽  
Self Esteem ◽  
Secondary Outcome ◽  
Intervention Effect ◽  
Produce Change ◽  
Attribution Biases ◽  
Carry Over ◽  
Implicit And Explicit ◽  
Reasoning Biases

Abstract. Cognitive-behavioral therapy for psychosis (CBTp) builds on theoretical models that postulate reasoning biases and negative self-schemas to be involved in the formation and maintenance of delusions. However, it is unclear whether CBTp induces change in delusions by improving these proposed causal mechanisms. This study reports on a mediation analysis of a CBTp effectiveness trial in which delusions were a secondary outcome. Patients with psychosis were randomized to individualized CBTp (n = 36) or a waiting list condition (WL; n = 34). Reasoning biases (jumping to conclusions, theory of mind, attribution biases) and self-schemas (implicit and explicit self-esteem; self-schemas related to different domains) were assessed pre- and post-therapy/WL. The results reveal an intervention effect on two of four measures of delusions and on implicit self-esteem. Nevertheless, the intervention effect on delusions was not mediated by implicit self-esteem. Changes in explicit self-schemas and reasoning biases did also not mediate the intervention effects on delusions. More focused interventions may be required to produce change in reasoning and self-schemas that have the potential to carry over to delusions.

Investigation on the control of multiphase flow behavior in a continuous casting tundish during ladle change

2020 ◽  
Vol 117 (6) ◽  
pp. 619
Author(s):  
Rui Xu ◽  
Haitao Ling ◽  
Haijun Wang ◽  
Lizhong Chang ◽  
Shengtao Qiu
Keyword(s):  
Multiphase Flow ◽  
Flow Behavior ◽  
Rolled Strip ◽  
Impact Zone ◽  
Steel Cleanliness ◽  
Carry Over ◽  
Slag Carry Over ◽  
Hot Rolled ◽  
Turbulence Inhibitor ◽  
The Impact

The transient multiphase flow behavior in a single-strand tundish during ladle change was studied using physical modeling. The water and silicon oil were employed to simulate the liquid steel and slag. The effect of the turbulence inhibitor on the slag entrainment and the steel exposure during ladle change were evaluated and discussed. The effect of the slag carry-over on the water-oil-air flow was also analyzed. For the original tundish, the top oil phase in the impact zone was continuously dragged into the tundish bath and opened during ladle change, forming an emulsification phenomenon. By decreasing the liquid velocities in the upper part of the impact zone, the turbulence inhibitor decreased considerably the amount of entrained slag and the steel exposure during ladle change, thereby eliminating the emulsification phenomenon. Furthermore, the use of the TI-2 effectively lowered the effect of the slag carry-over on the steel cleanliness by controlling the movement of slag droplets. The results from industrial trials indicated that the application of the TI-2 reduced considerably the number of linear inclusions caused by ladle change in hot-rolled strip coils.

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