Aminopeptidase activities in Peptostreptococcus spp. are statistically correlated to gelatin hydrolysis

1998 ◽  
Vol 44 (3) ◽  
pp. 303-306 ◽  
Author(s):  
James Ng ◽  
Lai-King Ng ◽  
Denis Mayrand ◽  
Jo-Anne R. Dillon
Keyword(s):  
Gelatin Hydrolysis

Occurrence of Bacillus cereus and the Bacteriological Quality of Chinese “Take-Out” Foods

1978 ◽  
Vol 41 (6) ◽  
pp. 450-454 ◽  
Author(s):  
D. A. SCHIEMANN
Keyword(s):  
Bacillus Cereus ◽  
Nitrate Reduction ◽  
Biochemical Characterization ◽  
Bacteriological Examination ◽  
Bacteriological Quality ◽  
Litmus Milk ◽  
Aerobic And Anaerobic ◽  
Gelatin Hydrolysis

One hundred sixty-five samples of various foods were collected from 24 different Chinese take-out restaurants for bacteriological examination which included enumeration of Bacillus cereus by three media, MYP, KG and blood agars. Blood agar was less selective but no quantitative differences in recovery were apparent. Twenty-eight samples (15%) yielded B. cereus in excess of 100 per gram, and 20 of these were fried rice (33% positive), which also showed the poorest overall bacteriological quality. Biochemical characterization of 232 isolates of B. cereus showed 96% or more positive for catalase, nitrate reduction, beta-haemolysis, subterminal-ellipsoidal spores, aerobic and anaerobic utilization of glucose, Voges-Proskauer, fermentation of glycerol, gelatin hydrolysis, and alkaline peptonization of litmus milk; and a negative reaction in mannitol. Variable results were obtained for motility, fermentation of sucrose and salicin, and starch hydrolysis. Thirty-three isolates were susceptible to 12 of 19 antibiotics tested, and resistant to colistin. Six (18%) were susceptible to penicillin.

Direct Testing of Gelatin Hydrolysis in Rapid Perfringens Medium

1983 ◽  
Vol 66 (4) ◽  
pp. 1045a-1047
Author(s):  
Marilyn Smith ◽  
Thomas J Mood
Keyword(s):  
Clostridium Perfringens ◽  
Related Species ◽  
Detection System ◽  
Food Samples ◽  
Direct Testing ◽  
Litmus Milk ◽  
Gelatin Hydrolysis

Abstract Rapid perfringens medium (RPM) was previously shown to be effective for detection of low numbers of Clostridium perfringens in foods. The detection system of RPM is based on a stormy fermentation of litmus milk. RPM also contains 6% gelatin, and the present work was performed to determine if gelatin hydrolysis can be tested directly in this medium. Twenty-three strains of C. perfringens and related species were inoculated into tubes of RPM and lactose gelatin. All strains were positive for stormy fermentation on RPM after 24 h incubation at 46°C. Seventeen exhibited gelatin hydrolysis on both RPM and lactose gelatin. Tubes of RPM inoculated with slurries of food samples spiked with each of 11 strains of C perfringens were positive for stormy fermentation and gelatin liquefaction. Most C. perfringens hydrolyze gelatin, so this test augments stormy fermentation in RPM as an additional indicator for the presence of this pathogen.

scholarly journals Biochemical and Molecular Analysis ofStaphylococcus aureusClinical Isolates from Hospitalized Patients

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Amit Karmakar ◽  
Parimal Dua ◽  
Chandradipa Ghosh
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Hospitalized Patients ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Biochemical Tests ◽  
Species Specific ◽  
High Level ◽  
Gelatin Hydrolysis

Staphylococcus aureusis opportunistic human as well as animal pathogen that causes a variety of diseases. A total of 100Staphylococcus aureusisolates were obtained from clinical samples derived from hospitalized patients. The presumptiveStaphylococcus aureusclinical isolates were identified phenotypically by different biochemical tests. Molecular identification was done by PCR using species specific 16S rRNA primer pairs and finally 100 isolates were found to be positive asStaphylococcus aureus. Screened isolates were further analyzed by several microbiological diagnostics tests including gelatin hydrolysis, protease, and lipase tests. It was found that 78%, 81%, and 51% isolates were positive for gelatin hydrolysis, protease, and lipase activities, respectively. Antibiogram analysis of isolatedStaphylococcus aureusstrains with respect to different antimicrobial agents revealed resistance pattern ranging from 57 to 96%. Our study also shows 70% strains to be MRSA, 54.3% as VRSA, and 54.3% as both MRSA and VRSA. All the identified isolates were subjected to detection ofmecA,nuc, andhlbgenes and 70%, 84%, and 40% were found to harbourmecA,nuc, andhlbgenes, respectively. The current investigation is highly important and informative for the high level multidrug resistantStaphylococcus aureusinfections inclusive also of methicillin and vancomycin.

X-ray Film Used for Gelatin Hydrolysis: Comparison with Standard Methods

1976 ◽  
Vol 7 (9) ◽  
pp. 33-34 ◽  
Author(s):  
Stephen C. Edberg ◽  
M. Helen Slater ◽  
Ervin Melton ◽  
Jacques M. Singer
Keyword(s):  
Standard Methods ◽  
X Ray ◽  
Gelatin Hydrolysis

THE INFLUENCE OF GELATIN HYDROLYSIS ON DIFFUSION THROUGH GLYCEROGELATIN GELS

1990 ◽  
Vol 42 (S1) ◽  
pp. 4P-4P ◽  
Author(s):  
N. Anthony Armstrong ◽  
Tsige G. Mariam ◽  
R. Christian Moreton ◽  
Helen J. Thompson
Keyword(s):  
Gelatin Hydrolysis

scholarly journals Effect of ultrasonic pretreatment on kinetics of gelatin hydrolysis by collagenase and its mechanism

2016 ◽  
Vol 29 ◽  
pp. 495-501 ◽  
Author(s):  
Zhi-Long Yu ◽  
Wei-Cai Zeng ◽  
Wen-Hua Zhang ◽  
Xue-Pin Liao ◽  
Bi Shi
Keyword(s):  
Ultrasonic Pretreatment ◽  
Kinetics Of ◽  
Gelatin Hydrolysis

scholarly journals RAPID METHODS FOR THE DETECTION OF GELATIN HYDROLYSIS, 12

1959 ◽  
Vol 77 (1) ◽  
pp. 60-64 ◽  
Author(s):  
Joseph J. McDade ◽  
R. H. Weaver
Keyword(s):  
Rapid Methods ◽  
Gelatin Hydrolysis

scholarly journals Recovery of Silver from Waste X-Ray Film by Alkaline Protease from Conidiobolus coronatus

1970 ◽  
Vol 6 (1) ◽  
pp. 60-69 ◽  
Author(s):  
S Shankar ◽  
SV More ◽  
R Seeta Laxman
Keyword(s):  
Alkaline Protease ◽  
Proteolytic Enzymes ◽  
Enzyme Concentration ◽  
Metallic Silver ◽  
Silver Recovery ◽  
X Ray ◽  
Gelatin Layer ◽  
Engineering And Technology ◽  
Hydrolysis Of ◽  
Gelatin Hydrolysis

The waste X-ray/ photographic films contain 1.5 - 2 % (w/w) black metallic silver which isrecovered and reused. Around 18-20% of the world's silver needs are supplied by recyclingphotographic waste. Since silver is linked to gelatin in the emulsion layer, it is possible to break thesame and release the silver using proteolytic enzymes. Alkaline protease from Conidioboluscoronatus was investigated for enzymatic hydrolysis of gelatin from waste X-ray films. At the endof the treatment, gelatin layer was completely removed leaving the polyester film clean and silverwas recovered in the hydrolysate, both of which can be reused. Various parameters such as pH,temperature, enzyme concentration, time etc on silver removal from the film were studied. Gelatinhydrolysis was monitored by measuring increase in turbidity in the hydrolysate, which wasaccompanied by release of protein and hydroxyproline. Gelatin layer was stripped completelywithin 6 min with 1.35 U ml-1 of protease at 40°C, pH 10. Rate of gelatin hydrolysis increased withincreased in protease concentration. The enzyme could be effectively reused for four cycles ofgelatin hydrolysis. Silver in hydolysate was around 3.87% (w/w) based on total weight of sludge.Key words: Silver recovery; X-ray films; gelatin hydrolysis; alkaline protease; Conidiobolus coronatusDOI: 10.3126/kuset.v6i1.3311 Kathmandu University Journal of Science, Engineering and Technology Vol.6(1) 2010, pp60-69

A New Approach for Differentiating Flexibacteria Isolated from Cold-water and Warmwater Fish

1980 ◽  
Vol 37 (6) ◽  
pp. 1040-1042 ◽  
Author(s):  
Stephen W. Pyle ◽  
Emmett B. Shotts Jr.
Keyword(s):  
Cold Water ◽  
Fish Disease ◽  
Biochemical Tests ◽  
New Approach ◽  
Water Fish ◽  
Warmwater Fish ◽  
Gelatin Hydrolysis ◽  
Biochemical Differentiation

Seventeen strains of flexibacteria associated with fish disease were evaluated with the API 20E system of biochemical tests. The results differentiate between isolates from warmwater fish, which utilize arabinose, melibiose, and sucrose; and isolates from cold-water fish, which are negative on these tests. Cold-water fish isolates may be further divided into subgroups on the basis of gelatin hydrolysis. The API system appears to provide the first biochemical differentiation of this group of poorly defined organisms.Key words: API 20E, flexibacteria, differentiate

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