A comparison of the heat shock response in juvenile and adult rainbow trout (Oncorhynchus mykiss) — implications for increased thermal sensitivity with age

2009 ◽  
Vol 66 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Stephanie L. Fowler ◽  
Diana Hamilton ◽  
Suzanne Currie
Keyword(s):  
Rainbow Trout ◽  
Heat Shock ◽  
Oncorhynchus Mykiss ◽  
Heat Shock Response ◽  
Thermal Sensitivity ◽  
Life Stage ◽  
Juvenile Fish ◽  
Early Life Stage ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Shock Response

We conclude that the heat shock response is enhanced in juvenile compared with adult rainbow trout, Oncorhynchus mykiss . We have shown that the magnitude of the inducible heat shock response, as measured by increases in the heat shock protein (Hsp) Hsp70 is significantly greater in the hearts of fingerling than in adult trout following an acute (1 h) heat stress at 25 °C. Juvenile fish also express higher levels of constitutive Hsps (Hsc70, Hsp90) in their tissues, possibly reflecting higher rates of protein synthesis and increased chaperone function in this early life stage. This robust heat shock response in juvenile trout may contribute to a greater thermal resistance that is often observed in young salmonid fish.

scholarly journals Effects of heat stress on Hsp90a2b and Hsp10 mRNA expression and blood cell apoptosis in rainbow trout Oncorhynchus mykiss (Walbaum, 1972)

2019 ◽  
Vol 66 (4) ◽  
Author(s):  
Zhicheng Luo ◽  
Yanjing Zhou ◽  
Zhe Liu ◽  
Binpeng Xia ◽  
Yongjie Wang
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Heat Shock ◽  
Blood Cell ◽  
Oncorhynchus Mykiss ◽  
Blood Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Shock Response ◽  
Heat Shock Protein Genes ◽  
Increasing Temperature

To study the response to heat stress in rainbow trout Oncorhynchus mykiss (Walbaum, 1972), the expression of heat shock protein genes Hsp90a2b and Hsp10 in selected tissues and apoptosis of blood cells were investigated at water temperatures of 18, 21, 23, 24, 25 and 26°C. Expressions of Hsp90a2b in the gill, mid-kidney, brain, heart and muscle were generally upregulated with increasing temperature. Hsp90a2b expression in the liver increased rapidly at 21°C and then showed a significant decrease and finally a significant increase at 25°C and 26°C (p<0.05). Hsp10 expression showed an irregular but overall increasing tendency in gill, spleen and mid-kidney and a wave-like increasing pattern in liver, heart and muscle. Levels of Hsp90a2b at 25°C and Hsp10 at 26°C in all tissues were always significantly higher compared to 18°C (p<0.05). The apoptosis rate at 21°C was significantly higher than that at 18°C, followed by a fall and was the highest at 25°C. All these data indicated that the heat shock response already occurred at 21°C which induced expressions of Hsp90a2b and Hsp10 as well as blood cells apoptosis and 25°C may be a key temperature for stress response in rainbow trout.

Synthesis of stress protein 70 (Hsp70) in rainbow trout (Oncorhynchus mykiss) red blood cells

1997 ◽  
Vol 200 (3) ◽  
pp. 607-614 ◽  
Author(s):  
S Currie ◽  
B Tufts
Keyword(s):  
Protein Synthesis ◽  
Rainbow Trout ◽  
Heat Shock ◽  
Oncorhynchus Mykiss ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Stress Proteins ◽  
Stress Protein ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Hsp70 Synthesis

Unlike enucleated mammalian red blood cells (rbcs), the nucleated rbcs of lower vertebrates are capable of protein synthesis and may, therefore, serve as a valuable model to investigate the adaptive significance of stress protein synthesis in cells. This study examined the synthesis of stress protein 70 (Hsp70) in rbcs of the temperature-sensitive rainbow trout Oncorhynchus mykiss in response to heat shock and anoxia. Through western blot analysis, we have demonstrated that rainbow trout rbcs synthesize Hsp70 both constitutively and in response to an increase in temperature. Radioisotopic labelling experiments indicated that the temperature at which Hsp70 synthesis was induced in fish acclimated to 10 &deg;C was between 20 and 25 &deg;C. Actinomycin D blocked de novo Hsp70 synthesis, implying that synthesis of Hsp70 is regulated at the level of transcription in rainbow trout rbcs. Since trout rbcs rely heavily on aerobic metabolism, but may also experience very low oxygen levels within the circulation, we also examined the relative importance of (1) anoxia as a stimulus for Hsp70 synthesis and (2) oxygen as a requirement for protein synthesis under control and heat-shock conditions. We found that trout rbcs were capable of protein synthesis during 2 h of anoxia, but did not increase Hsp70 synthesis. Moreover, rbcs subjected to combined anoxia and heat shock exhibited increases in Hsp70 synthesis that were similar in magnitude to those in cells exposed to heat shock alone. The latter results suggest that rainbow trout rbcs are (1) able to synthesize non-stress proteins during anoxia, (2) capable of tolerating periods of reduced oxygen availability without increased synthesis of stress proteins and (3) able to maintain the integrity of their heat-shock response even during periods of anoxia.

Alarm cue specificity and response ontogeny in juvenile rainbow trout (Oncorhynchus mykiss)

Behaviour ◽  
2017 ◽  
Vol 154 (3) ◽  
pp. 377-385 ◽  
Author(s):  
Marianna E. Horn ◽  
Douglas P. Chivers
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Chemical Cues ◽  
Life Stage ◽  
Age Groups ◽  
Alarm Cues ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Risk Of Predation ◽  
Threat Cues ◽  
Two Age Groups

Many aquatic prey animals release chemical cues upon being captured by a predator. These chemical cues, referred to as alarm cues, may act to warn nearby individuals of danger. For the cues to be useful, fish must be able to discern if they are indicative of a real threat; cues from conspecifics in different age groups may be irrelevant due to size- and habitat-related shifts in predation risk. We test the response of newly-hatched rainbow trout,Oncorhynchus mykiss, to three concentrations of alarm cues from conspecifics from two age groups: newly-hatched versus six-month-old juveniles. Newly-hatched trout demonstrated a significant fright response to all three concentrations of alarm cues, but showed no difference in strength of response based on either concentration or age of the cue donor. We propose that the newly-hatched trout did not respond differently because of the high risk of predation that they face during this life stage.

Sign in / Sign up

Export Citation Format

Share Document