Dissolution of Fish Muscle Homogenates by Lysolecithin

On incubating skeletal muscle homogenates from rainbow trout with lysolecithin (LL) and comparing them with homogenates of the same muscle without added LL, and after centrifuging the mixtures, it was found that the N content of the supernatant solution of the homogenate containing LL was about 20% higher than that of the homogenate without LL. Increases close to maximum in N content of the supernatant solution were found to occur at a concentration of about 4 mg LL per ml of incubation medium containing 100 mg muscle in 3.0 ml of 0.9% NaCl at a pH of 6.0–8.0 and at about 35 C for a period of 1 hr. Snake venom phospholipase A added to muscle homogenate showed no solubilizing activity and α-tocopherol acetate and cortisol showed irregular stimulation. It was concluded that LL exerts a solubilizing action on fish muscle homogenates.

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Effect of Hyperthermia on Proteases and Growth Regulators in the Skeletal Muscle of Cultivated Rainbow Trout O. mykiss

Russian Journal of Bioorganic Chemistry ◽

10.1134/s1068162020060114 ◽

2020 ◽

Vol 46 (6) ◽

pp. 1181-1186

Author(s):

N. P. Kantserova ◽

M. V. Churova ◽

L. A. Lysenko ◽

E. D. Tushina ◽

M. A. Rodin ◽

...

Keyword(s):

Skeletal Muscle ◽

Rainbow Trout ◽

Growth Regulators

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Discovery of novel [Arg49]phospholipase A2 isozymes from Protobothrops elegans venom and regional evolution of Crotalinae snake venom phospholipase A2 isozymes in the southwestern islands of Japan and Taiwan

Toxicon ◽

10.1016/j.toxicon.2006.06.021 ◽

2006 ◽

Vol 48 (6) ◽

pp. 672-682 ◽

Cited By ~ 15

Author(s):

Takahito Chijiwa ◽

Emi Tokunaga ◽

Ryo Ikeda ◽

Koki Terada ◽

Tomohisa Ogawa ◽

...

Keyword(s):

Phospholipase A2 ◽

Snake Venom ◽

Snake Venom Phospholipase

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Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis

Biochemical Journal ◽

10.1042/bj1210817 ◽

1971 ◽

Vol 121 (5) ◽

pp. 817-827 ◽

Cited By ~ 74

Author(s):

R. C. Hider ◽

E. B. Fern ◽

D. R. London

Keyword(s):

Skeletal Muscle ◽

Amino Acids ◽

Protein Synthesis ◽

Amino Acid ◽

Incubation Medium ◽

Extensor Digitorum Longus ◽

Extensor Digitorum ◽

Longus Muscle ◽

Kinetics Of

1. The kinetics of radioactive labelling of extra- and intra-cellular amino acid pools and protein of the extensor digitorum longus muscle were studied after incubations with radioactive amino acids in vitro. 2. The results indicated that an extracellular pool could be defined, the contents of which were different from those of the incubation medium. 3. It was concluded that amino acids from the extracellular pool, as defined in this study, were incorporated directly into protein.

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Structure–activity relationship of non-coplanar polychlorinated biphenyls toward skeletal muscle ryanodine receptors in rainbow trout (Oncorhynchus mykiss)

Aquatic Toxicology ◽

10.1016/j.aquatox.2013.06.003 ◽

2013 ◽

Vol 140-141 ◽

pp. 204-212 ◽

Cited By ~ 18

Author(s):

Erika B. Fritsch ◽

Isaac N. Pessah

Keyword(s):

Skeletal Muscle ◽

Rainbow Trout ◽

Polychlorinated Biphenyls ◽

Oncorhynchus Mykiss ◽

Ryanodine Receptors ◽

Activity Relationship ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Structure Activity ◽

Coplanar Polychlorinated Biphenyls ◽

Relationship Of

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Properties of 5′-deiodinase of 3,3′,5′-triiodothyronine in rat skeletal muscle

Acta Endocrinologica ◽

10.1530/acta.0.1200069 ◽

1989 ◽

Vol 120 (1) ◽

pp. 69-74 ◽

Cited By ~ 4

Author(s):

Fujiko Tsukahara ◽

Teruko Nomoto ◽

Michiko Maeda

Keyword(s):

Skeletal Muscle ◽

Drinking Water ◽

Incubation Medium ◽

Marked Reduction ◽

Daily Administration ◽

Type I ◽

Rat Skeletal Muscle ◽

Thyroid Status ◽

Altered Thyroid

Abstract. To characterize rT3 5′-deiodinase (5′D) in rat skeletal muscle, the effects of altered thyroid status and PTU on rT3 5′D were studied. rT3 5′D activity was measured by incubating homogenates of rat skeletal muscle with [125]rT3, iodine labelled in the outer ring, in the presence of 20 mmol/l DL-dithiothreitol. This activity was observed to increase significantly 24 h after a single sc injection of T3 (75 μg/kg). The increase following the daily administration of this drug (15 or 75 μg/kg) for 3 and 14 days was dependent on the dose and number of previous days of injection. A significant decrease in activity was observed 2 weeks after thyroidectomy. The addition of 0.1 mmol/l 6-n-propyl-2-thiouracil (PTU) to the incubation medium in vitro caused a marked reduction in the activity in homogenates of skeletal muscle from hypothyroid, euthyroid and hyperthyroid rats. PTU, present at 0.05% in the drinking water for 2 weeks virtually abolished it. The properties of rT3 5′D in rat skeletal muscle thus appear to be essentially the same as those of type I enzyme with respect to response toward altered thyroid status and PTU.

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