Comparison of Changes in Trimethylamine, Dimethylamine, and Extractable Protein in Iced and Frozen Gadoid Fillets

1973 ◽  
Vol 30 (8) ◽  
pp. 1246-1248 ◽  
Author(s):  
C. H. Castell ◽  
W. E. Neal ◽  
J. Dale

When fillets of Atlantic cod (Gadus morhua), pollock (Pollachius virens), cusk (Brosme brosme), and silver hake (Merluccius bilinearis) were iced and the opposite fillets frozen, large amounts of trimethylamine were rapidly produced in the iced fish but none was formed in the frozen fish within 60 days. Hake produced large, similar amounts of dimethylamine (DMA) in iced and frozen fillets. The other three species produced more DMA in the frozen than in the iced fillets, but always much less than in the hake. In both frozen and iced fish the production of DMA was accompanied by a corresponding decrease in extractable protein nitrogen.

1973 ◽  
Vol 30 (8) ◽  
pp. 1205-1213 ◽  
Author(s):  
C. H. Castell ◽  
B. Smith ◽  
W. J. Dyer

Addition of formaldehyde to fresh cod muscle, to give concentrations of 10 to 200 ppm, brought about marked decreases in the extractable protein content during holding periods of 24 hr or less at 0 C. Similar levels of formaldehyde, produced during frozen storage of gadoid (Atlantic cod, Gadus morhua, pollock, Pollachius virens, cusk, Brosme brosme, and silver hake, Merluccius bilinearis), fillets at −5 C, brought about similar reductions in the extractable proteins. Comparative tests showed that, in the concentrations normally encountered in deteriorating frozen gadoid fillets, formaldehyde was a much more active protein-insolubilizing agent than free fatty acid. It is evident that in these protein changes more than one mechanism is involved. Observed species-differences in the extent to which fish proteins became insolubilized during storage appeared to be related to presence or absence of these different mechanisms. The more rapid and more extensive denaturation of most gadoid fillets in frozen storage than of fillets of nongadoid species appears to be directly related to the presence of muscle enzyme of the former group that is capable of producing formaldehyde from trimethylamine oxide, which is absent in the muscle of the nongadoid species so far tested.


1968 ◽  
Vol 25 (4) ◽  
pp. 733-755 ◽  
Author(s):  
W. A. MacCallum ◽  
June I. Jaffray ◽  
D. N. Churchill ◽  
D. R. Idler

Quality assessments, based on organoleptic evaluations and chemical tests, were conducted before and after cold storage, on trap-caught cod (Gadus morhua) which was unfrozen, once-frozen, or twice-frozen. The cod was processed both before and during rigor, after icing or after handling without ice, at various times during the catching season.Lactate determinations and pH measurements on whole fish at killing showed a seasonal variation. There was a correlation between lactate determinations and pH measurements in freshly killed and freshly frozen fillets that had been frozen both before and during rigor. Icing immediately after killing slowed the rate of lactate accumulation.Differences in the state of pre- and in-rigor chilled fish at freezing, as assessed by muscle glycogen, lactate, and reserves of ATP, resulted in differences in texture, thaw-drip, and pH. Patterns in texture may be predicted by the amounts of thaw-drip and the pH measured in the cold-stored pack.Intensity of mealiness and short-grained features in freshly frozen (once) fish was correlated with the level of ATP immediately before cooking. Fillets frozen before rigor toughened very slowly in storage at −23 C and the mealy condition eventually disappeared. Hence, fish downgraded for mealiness at freezing improved or maintained initial overall texture scores during 6 months in storage. Although the panel showed a clear preference for fish frozen pre-rigor, it would be impossible for industry to freeze more than a portion of the landings pre-rigor. To obtain the next best once-frozen product, chilled in-rigor fish should be processed.Double freezing gave the next best product from chilled, prerigor fish. Fish frozen pre-rigor in the "dressed" condition and stored at −23 C, then thawed, processed, and refrozen at 16 weeks, was equal in quality to once-frozen controls. The texture in the twice-frozen material prepared from iced, prerigor frozen fish dropped after a further 10 weeks storage, making it little better than the twice-frozen product of commercial-like handling. A very satisfactory twice-frozen product was obtained also by holding unfilleted blocks of non-iced, gutted fish at −46 C before thawing and refreezing, followed by remaining storage at −23 C. However, such low temperature storage is not within the scope of present commercial operations.With pre- and in-rigor frozen material a correlation was found between thaw-drip and pH, thaw-drip and overall texture (in-rigor freezing only), pH and overall texture values, and between pH and toughness. There was also a correlation between extractable protein nitrogen (EPN) values and overall texture scores of fish frozen in various stages of rigor (encountered during unfrozen storage of upwards of 24 hr before processing) but not between EPN and overall acceptability. With twice-frozen fillets prepared from material also handled unfrozen as above, there was significant correlation between EPN values at tasting and overall texture scores and between EPN and overall acceptability scores.Free fatty acid (FFA) values of the trap fish were high and showed no increase as the result of thawing and refreezing or further storage at −23 C or with pre- and in-rigor freezing. Storage at −46 C resulted in less FFA development. There was significant correlation between EPN and FFA values for June-caught once-frozen fish and between FFA values and strictly taste scores for June and July fish but no correlation between FFA and overall texture scores, or in the case of twice-frozen fish of any particular pre-freezing treatment, between FFA and taste, overall texture, or overall acceptability.


1977 ◽  
Vol 34 (12) ◽  
pp. 2369-2373 ◽  
Author(s):  
Doris Fraser Hiltz ◽  
D. H. North ◽  
Barbara Smith Lall ◽  
R. A. Keith

Refrozen silver hake (Merluccius bilinearis), processed as fillets and minced flesh after thawing of stored round fish that had been frozen within 14 h of capture, underwent rapid deterioration during storage at −18 °C compared with once-frozen control materials from the same lot of fish. The estimated maximum storage life of silver hake refrozen as fillets after 3 and 6 mo storage of the round fish at −25 °C was reduced to about 4.5 and 1 mo, respectively, from 10 mo for once-frozen control fillets. Quality of the refrozen materials immediately after thawing and refreezing was similar to that of the round-frozen fish, except after 6 mo, where some initial deterioration occurred, particularly in minced flesh. Minced flesh was more unstable in frozen storage than fillets. In all once- and twice-frozen materials, formation of dimethylamine occurred concomitantly with decrease in protein extractability. Round-frozen fish underwent no loss in protein extractability during 6 mo storage at −25 °C, but some lipid hydrolysis occurred. These results suggest that the freeze–thaw–refreeze process as applied to silver hake will yield a final product of acceptable quality provided that storage of the round fish does not exceed 3–4 mo and that the refrozen materials are marketed within a month after processing. Key words: silver hake, Merluccius bilinearis, refrozen storage, dimethylamine, minced flesh


1987 ◽  
Vol 44 (10) ◽  
pp. 1729-1736 ◽  
Author(s):  
Christopher T. Taggart ◽  
Kenneth T. Frank

Variations in the occurrence of Oikopleura spp. were strongly linked to the wind field and ensuing water temperatures in an inshore region of eastern Newfoundland during June–August of 1979 and 1981–83. Oikopleura foul inshore fishing gear with their discarded houses ("slub") and fluctuate in abundance from day to day as a function of wind-driven upwelling. Densities of Oikopleura in cold upwelling water during four years ranged between 20 and 800/m3. Oikopleura densities < 1/m3 occurred when upwelling ceased and warmer water occupied the inshore region. A simple model incorporating a daily averaged wind vector, Julian day, and growing degree-day explained 62% of the daily variation in Oikopleura abundance in 1979. The model yielded density estimates that were significantly (p < 0.05) correlated with observed densities in each of the other three years. We provide evidence that net fouling by Oikopleura in the inshore and maximum Atlantic cod (Gadus morhua) catch is coincident and suggest that an annual hindcast index of slub intensity may help determine the impact of slub conditions on the inshore fishery.


1967 ◽  
Vol 24 (6) ◽  
pp. 1315-1375 ◽  
Author(s):  
Khwaja Abdul Mujib

The cranial skeletons of four gadid fishes, Gadus morhua (subfamily Gadinae), Urophysis chuss (Lotinae), Lota lota (Lotinae), and Merluccius bilinearis (Merluccinae), are described in detail.The Merluccinae probably gave rise, on one hand, to the subfamily Lotinae and, on the other, to the subfamily Gadinae; both of these subfamilies became further specialized. All three subfamilies differ from each other in their cranial osteology, as well as in other characters.


1999 ◽  
Vol 56 (11) ◽  
pp. 2069-2077 ◽  
Author(s):  
B Planque ◽  
T Frédou

Variability in the recruitment of fish has been attributed to either changes in the environment or variations in the size of reproductive stocks. Disentangling the effects of environment and stock has proven to be problematic and has resulted in recurrent controversy between studies supporting either hypothesis. In the present study, we examine the relationship between interannual changes in temperature and variation in recruitment for nine Atlantic cod (Gadus morhua) stocks in the North Atlantic. We show that for individual stocks, the relationship often appears weak and statistically not significant. On the other hand, by combining in a single metaanalysis the results from individual stocks, we demonstrate that recruitment of Atlantic cod is linked to interannual fluctuations in temperature in such a way that for stocks located in warm water the relationship is negative, for stocks located in cold water the relationship is positive, and there is no relationship for stocks located in the middle of the temperature range.


1976 ◽  
Vol 33 (11) ◽  
pp. 2560-2567 ◽  
Author(s):  
Doris Fraser Hiltz ◽  
Barbara Smith Lall ◽  
D. W. Lemon ◽  
W. J. Dyer

During frozen storage at −10 C, deterioration in muscle of silver hake (Merluccius bilinearis) was marked by rapid and extensive production of dimethylamine, concomitant decrease in extractable protein, and by lipid hydrolysis. Evidence of lipid oxidation in this gadoid species of relatively high fat content (2–4%) was also obtained. In minced flesh the rates of deterioration were about twice as fast as in fillets. Holding round fish for up to 6 days in refrigerated sea water (RSW) at 0–1 C before processing extended the frozen storage life of fillets at −10 C by 2–3 wk and of minced flesh by 1 wk over that for comparable materials prepared from round fish held in ice. Materials prepared from winter (March) and summer (August) fish showed little or no difference in rates of deterioration. The susceptibility of silver hake to deterioration at −10 C is similar to cusk; deterioration is faster than in cod or haddock, but not as fast as in red hake (Urophycis chuss). In all silver hake materials negligible deterioration occurred during frozen storage at −26 C for up to 6 mo.During preprocessing storage of round silver hake in RSW, a firm texture and acceptable appearance were retained for several days longer than in round fish held in ice, where objectionable softening of the flesh occurred, particularly in summer-caught fish. Saturation of the sea water with CO2 retarded the onset of bacterial spoilage in RSW-held fish, which otherwise developed more rapidly than in iced fish.


Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1515
Author(s):  
Frederik Feldmann ◽  
Alba Ardura ◽  
Carmen Blanco-Fernandez ◽  
Eva Garcia-Vazquez

Atlantic cod, Gadus morhua, is a highly appreciated fish in European seafood markets and is one of the most substituted fish species in the world. Fraud have been detected in European markets in the last decade, finding different substitute species sold as G. morhua or Atlantic cod on the label. In this study, we analyzed 252 samples of fresh and frozen cod fillets sold in Germany, the Netherlands, and France using DNA barcoding. Different trends were found in different countries: while the level of mislabeling found in Germany and the Netherlands remained at zero in the last years, a significant increase was found in the French markets comparing the current results with previous studies on fillets in France. On the one hand, this mislabeling proves the need to encourage European efforts to control seafood authenticity; on the other, zero mislabeling in two countries shows the success of current European regulations.


1974 ◽  
Vol 52 (6) ◽  
pp. 777-782 ◽  
Author(s):  
G. J. D. Smith ◽  
D. E. Gaskin

Stomach contents of 81 harbor porpoises (Phocoena phocoena (L.)) collected from eastern Canadian coastal waters during 1969–1972 were examined. A total of 454 free otoliths and 54 undigested specimens representing a minimum of 281 fish, consisting of nine species in seven families, were recovered from the copious semidigested fish remains found in 52 of the stomachs. Clupea harengus (herring), Gadus morhua (cod), and Scomber scombrus (mackerel) otoliths accounted for more than 78% of the total. Osmerus mordax (smelt), Pollachius virens (pollock), Merluccius bilinearis (silver hake), Sebastes marinus (redfish), and Macrozoarces americanus (ocean pout) otoliths were also identified. Squid (Illex sp.), hagfish (Myxine glutinosa), and polychaetes (Nereis sp.) were identified in a few stomachs. Fifteen of the stomachs examined were empty or contained only fluid. Examination of the stomachs of the 16 smallest animals indicated that weaning occurred at body lengths of 100–104 cm.


2014 ◽  
Vol 514 ◽  
pp. 217-229 ◽  
Author(s):  
HY Wang ◽  
LW Botsford ◽  
JW White ◽  
MJ Fogarty ◽  
F Juanes ◽  
...  

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