Genetic Relationships among Populations of Alaskan Chinook Salmon (Oncorhynchus tshawytscha)

1987 ◽  
Vol 44 (4) ◽  
pp. 765-774 ◽  
Author(s):  
A. J. Gharrett ◽  
S. M. Shirley ◽  
G. R. Tromble
Keyword(s):  
Chinook Salmon ◽  
Gel Electrophoresis ◽  
Oncorhynchus Tshawytscha ◽  
Genetic Relationships ◽  
Starch Gel Electrophoresis ◽  
The South ◽  
Protein Coding ◽  
Starch Gel

Chinook salmon (Oncorhynchus tshawytscha) collected from 13 Alaskan drainages were genetically characterized at 28 protein coding loci using starch–gel electrophoresis. Chinook salmon in western Alaska are generally quite similar to each other but are distinct from the more diverse southeastern Alaskan populations. Genetic compositions of southeastern Alaskan populations are generally intermediate between those of western Alaska and previously studied non-Alaskan populations to the south. Given that chinook salmon survived the Wisconsin glaciation in both the Bering and Pacific refuges, we propose that chinook salmon from both refuges participated in the post-Wisconsin colonization of southeastern Alaskan rivers.

A Genetic Comparison of Sympatric Populations of Sand Lance (Genus Ammodytes) from the Region East of Cape Soya, Japan

1989 ◽  
Vol 46 (11) ◽  
pp. 1945-1951 ◽  
Author(s):  
Hiroaki Okamoto
Keyword(s):  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Distinct Species ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Sympatric Populations ◽  
Sand Lance ◽  
Protein Coding ◽  
Starch Gel ◽  
Genetic Comparison

Sand fance (Genus Ammodytes) collected from four stations off Japan and one station at Kodiak, Alaska were genetically characterized at 17 protein coding loci using starch-gel electrophoresis. Sand lance in Wakkanai (Cape Soya, Japan) consist of two genetically distinct groups. They are fixed for different alleles at four loci (Ldh-2, -3, G3pdh-2, and Mdhp-2). The genetic structure of one of the groups (Wakkanai-a group, W-a) is similar to that of A. personatus around Japan. The other group (Wakkanai-b group, W-b) has different genetic structure from either A. personatus or the Alaskan collection, which is presumed to belong to A. hexapterus. It is not presently possible to identify the affiliation of the W-b group; however, despite its sympatry with the W-a group, it is reproductively isolated and therefore is probably a distinct species occurring northeast of Hokkaido.

GENETIC RELATIONSHIPS AMONG REPRESENTATIVE POPULATIONS OF FIVE CHORISTONEURA SPECIES: C. OCCIDENTALIS, C. RETINIANA, C. BIENNIS, C. LAMBERTIANA, AND C. FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)

1981 ◽  
Vol 113 (9) ◽  
pp. 857-865 ◽  
Author(s):  
M. W. Stock ◽  
P. J. Castrovillo
Keyword(s):  
British Columbia ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Interspecific Variation ◽  
Genetic Distances ◽  
Starch Gel Electrophoresis ◽  
Separate Species ◽  
Individual Gene ◽  
Starch Gel

AbstractThe genetic make-up of representative populations of five Choristoneura species was compared using starch gel electrophoresis. Species included C. occidentalis Freeman from Idaho, C. biennis Freeman from British Columbia, C. retiniana (Walsingham) (= C. viridis Freeman) from Oregon, C. lambertiana ponderosana Obraztsov from Colorado, and C. fumiferana (Clemens) from Maine. When variation at individual gene loci was examined, intraspecific variation was often as great, and sometimes greater, than interspecific variation and few significant differences were noted among the species. The highest levels of overall genetic similarity occurred among C. occidentalis, C. biennis, and C. retiniana. Relatively greater genetic distances were found between this group and C. lambertiana and C. fumiferana. C. fumiferana was most distantly related to all other groups. Genetic identity values fell within the range more commonly associated with conspecific populations rather than with separate species.

Allozyme relationships of some frogs (genus Rana) from Yunnan, China

1989 ◽  
Vol 10 (3) ◽  
pp. 267-275 ◽  
Author(s):  
Timothy F. Sharbel ◽  
David M. Green ◽  
Keyword(s):  
Gel Electrophoresis ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
Starch Gel Electrophoresis ◽  
Eastern Siberia ◽  
Representative Species ◽  
Isozyme Loci ◽  
Starch Gel ◽  
Species Groups ◽  
Very High

AbstractThe genetic relationships of four species of frogs, genus Rana, from Yunnan, China, were investigated using starch gel electrophoresis of isozymes. Rana chaochiaoensis, R. pleuraden, R. phrynoides and R. limnocharis, representing four different species groups, were compared to each other and to representative species from other parts of Asia: R. amurensis from eastern Siberia, R. japonica from Japan, and R. nigromaculata from Korea. Twenty-four isozyme loci were reliably detected and scored, of which all but one were variable. R. chaochiaoensis, R. japonica and R. amurensis, all members of the Eurasian "brown frog" group, clustered together as a group although genetic distances were comparatively high. The "pond frogs", R. nigromaculata and R. pleuraden, similarly clustered together. Both R. phrynoides and R. limnocharis were highly divergent from the other species, but R. phrynoides seemed to be more closely affiliated with the "pond frogs" than with the "brown frogs". The data indicate that the highly enigmatic 64-chromosome karyotype of R. phrynoides arose from among 26-chromosome ancestors. The relationships of R. limnocharis cannot be precisely defined. Genetic divergence among Asiatic Rana appears to be very high.

scholarly journals Genetic Relationships among Weedy Purple Loosestrife (Lytrhum salicaria L.) Populations and Cultivars

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 856F-856
Author(s):  
Mark S. Strefeler ◽  
Elizabeth Darmo ◽  
Roger L. Becker ◽  
Elizabeth J. Katovich
Keyword(s):  
Cluster Analysis ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Geographic Location ◽  
Purple Loosestrife ◽  
Starch Gel Electrophoresis ◽  
Plant Proteins ◽  
Perennial Plant ◽  
Starch Gel

Starch gel electrophoresis of plant proteins was used to genetically identify purple loosestrife (Lythrum spp.) cultivars and weedy populations. Preliminary determinations were made as to what degree weedy loosestrife populations were related (or genetically similar) to populations of L. alatum, L. virgatum, and horticultural cultivars. Cluster analysis of the data indicated that native L. alatum was genetically different from all populations of purple loosestrife and cultivars examined. The L. salicaria and L. virgatum cultivars, as groups, were not genetically distinguishable from the weedy populations analyzed. Seven cultivars of L. salicaria origin analyzed as a group were not distinguishable from the eight cultivars of L. virgatum origin, indicating that separation by cultivar origin may not be feasible. While the two “groups” were not distinguishable, most individual cultivars could be distinguished from one another by isozyme phenotype. Genetic variation was high within populations of weedy purple loosestrife but low among populations, which is characteristic of polyploid, perennial plant species that are widely distributed. Geographic location did not consistently correlate with genetic similarity.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals PROTEIN POLYMORPHISMS, SEGREGATION IN GENETIC CROSSES AND GENETIC DISTANCES AMONG FISHES OF THE GENUS XIPHOPHORUS (POECILIIDAE)

Genetics ◽  
1982 ◽  
Vol 102 (3) ◽  
pp. 539-556
Author(s):  
Don C Morizot ◽  
Michael J Siciliano
Keyword(s):  
Goodness Of Fit ◽  
Inbred Strains ◽  
Interspecific Backcross ◽  
Genetic Distances ◽  
Rapid Expansion ◽  
Starch Gel Electrophoresis ◽  
Lower Vertebrate ◽  
Protein Coding ◽  
Group I ◽  
Starch Gel

ABSTRACT The products of 49 protein-coding loci were examined by starch gel electrophoresis for populational variation in six species of Xiphophorus fishes and/or segregation in intra- and interspecific backcross and intercross hybrids. Electrophoretic variation was observed for 29 of the 35 locus products in a survey of 42 population samples. The highest frequency of polymorphic loci observed in noninbred populations was 0.143. After ten or more generations of inbreeding, all loci studied were monomorphic. Inbred strains generally exhibited the commonest electrophoretic alleles of the population from which they were derived. An assessment of genetic distances among Xiphophorus populations reflected classical systematic relationships and suggested incipient subspeciation between X. maculatus from different drainages as well as several species groups. Thirty-three loci were analyzed with respect to segregation in hybrids. The goodness of fit of segregations to Mendelian expectations at all loci analyzed (except loci in linkage group I) is interpreted as evidence for high genetic compatibility of the genomes of Xiphophorus species. It is anticipated that these data will result in a rapid expansion of the assignment of protein-coding loci to linkage groups in these lower vertebrate species.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

STARCH GEL ELECTROPHORESIS OF MYOGEN FROM CHICKEN BREAST MUSCLE IN CONSTANT AND GRADIENT BUFFER SYSTEMS

1963 ◽  
Vol 41 (1) ◽  
pp. 369-387 ◽  
Author(s):  
J. M. Neelin
Keyword(s):  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Breast Muscle ◽  
Chicken Breast ◽  
Starch Gel Electrophoresis ◽  
Two Dimensional ◽  
Sodium Cacodylate ◽  
Gradient Systems ◽  
Optimal Separation ◽  
Starch Gel

By varying conditions of starch gel electrophoresis, factors contributing to the resolution of myogen proteins from chicken breast muscle have been studied. Variables examined included composition of the myogen extractant, protein concentration, ionic strength of electrophoretic media, pH of gel media, plane and direction of electrophoresis, and the nature of cations and anions in gel media and bridge solutions. The significance of anions was more closely studied with constant buffer systems, and gradient systems in which bridge electrolyte differed from, and gradually altered, the gel medium. Optimal separation was obtained in gradient systems with 0.10 M sodium chloride bridge solutions, and gel media of sodium cacodylate, pH 6.9, μ 0.010, which resolved 12 cationic zones, and sodium veronal, pH 7.4, μ 0.010, which resolved 10 anionic zones. These buffers in two-dimensional sequence revealed a total of about 24 components in this myogen.

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