A genetic linkage map of Silene vulgaris based on AFLP markers

Genome ◽  
2006 ◽  
Vol 49 (4) ◽  
pp. 320-327 ◽  
Author(s):  
Martin Bratteler ◽  
Christian Lexer ◽  
Alex Widmer

A genetic linkage map of an intraspecific cross between 2 Silene vulgaris s.l. ecotypes is presented. Three-hundred AFLP markers from 2 different restriction enzyme combinations were used to genotype an F2 mapping population. Maternal and paternal pure-coupling phase maps with 114 and 186 markers on 12 and 13 linkage groups, respectively, were constructed. Total map length of the paternal and maternal maps are 547 and 446 Kosambi cM, respectively. Nearly half of the markers (49%) exhibited significant transmission ratio distortion. Genome coverage and potential causes of the observed segregation ratio distortions are discussed. The maps represent a first step towards the identification of quantitative trait loci associated with habitat adaptation in the non-model species Silene vulgaris.Key words: AFLP, genome mapping, segregation distortion, Silene vulgaris.

2005 ◽  
Vol 110 (4) ◽  
pp. 669-677 ◽  
Author(s):  
A. Hamwieh ◽  
S. M. Udupa ◽  
W. Choumane ◽  
A. Sarker ◽  
F. Dreyer ◽  
...  

2009 ◽  
Vol 18 (5) ◽  
pp. 825-835 ◽  
Author(s):  
Wei-Ji Wang ◽  
Han-Ping Wang ◽  
Hong Yao ◽  
Geoff K. Wallat ◽  
Laura G. Tiu ◽  
...  

2010 ◽  
pp. 141-148 ◽  
Author(s):  
Y.S. Guo ◽  
C.J. Liu ◽  
Y.H. Zhao ◽  
J.H. Xie ◽  
P.R. Ren ◽  
...  

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Zhen-Xiang Lu ◽  
B Sosinski ◽  
G L Reighard ◽  
W V Baird ◽  
A G Abbott

A genetic linkage map for peach (Prunus persica (L.) Batsch) rootstocks has been constructed using amplified fragment length polymorphism (AFLP) markers in 55 F2 individuals derived from the cross Lovell x Nemared. From 21 different primer combinations, a total of 169 AFLP markers were scored, of which, 153 were assigned to 15 linkage groups covering 1297 centimorgans (cM) of the peach nuclear genome. The average interval between these markers was 9.1 cM. Two genes (Mi and Mij) involved in resistance to root-knot nematodes (Meloidogyne incognita (Kofoid and White) Chitwood and Meloidogyne javanica (Treub) Chitwood) were mapped to a single linkage group (Group I). These two loci were separated by a 16.5-cM interval. One codominant AFLP marker (EAA/MCAT10) was tightly linked to the Mij locus (3.4 cM), and a dominant AFLP marker (EAT/MCAT2) was found to be closely associated with the Mi locus (6.0 cM). These markers are being studied for utilization in peach rootstock breeding with marker-assisted selection.Key words: peach rootstocks, root-knot nematodes, resistance, AFLP, mapping.


Genome ◽  
2012 ◽  
Vol 55 (4) ◽  
pp. 327-335 ◽  
Author(s):  
Xiaoxia Yu ◽  
Xiaolei Li ◽  
Yanhong Ma ◽  
Zhuo Yu ◽  
Zaozhe Li

Using a population of 105 interspecific F2 hybrids derived from a cross between Agropyron mongolicum Keng and Agropyron cristatum (L.) Gaertn. ‘Fairway’ as a mapping population, a genetic linkage map of crested wheatgrass was constructed based on AFLP and RAPD molecular markers. A total of 175 markers, including 152 AFLP and 23 RAPD markers, were ordered in seven linkage groups. The map distance was 416 cM, with a mean distance of 2.47 cM between markers. The number of markers ranged from 13 to 46 in each linkage group and the length of groups ranged from 18 to 104 cM. The research found that 30 out of 175 molecular markers showed segregation distortion, accounting for 17% of all markers. This is the first genetic linkage map of crested wheatgrass. This map will facilitate gene localization, cloning, and molecular marker-assisted selection in the future.


Aquaculture ◽  
2012 ◽  
Vol 344-349 ◽  
pp. 66-81 ◽  
Author(s):  
Lei Liu ◽  
Jian Li ◽  
Ping Liu ◽  
Fazhen Zhao ◽  
Baoquan Gao ◽  
...  

Genome ◽  
2009 ◽  
Vol 52 (5) ◽  
pp. 438-446 ◽  
Author(s):  
Yasuhito Okuda ◽  
Shigeyuki Murakami ◽  
Teruyuki Matsumoto

In the cultivation of edible mushrooms, including Pleurotus pulmonarius (Fr.) Quel., the enormous number of spores produced by fruiting bodies can adversely affect mushroom growers’ health, mushroom cultivation facilities, and the genetic diversity of natural populations. In this study, we constructed a primary genetic linkage map and identified the locus associated with the sporulation-deficient (sporeless) mutation of P. pulmonarius using 150 progeny isolates derived from a cross between sporeless and wild-type isolates. Based on the segregation of 300 AFLP markers, two mating-type factors, and the sporeless trait, a linkage map was generated consisting of 12 linkage groups. The map covered a total genetic distance of 971 cM, with an average marker interval of 5.2 cM. The gene region responsible for the sporeless mutation was located in linkage group II including 40 AFLP markers and the A mating-type factor locus. Of these markers, the nearest marker to the sporeless locus was located 1.4 cM away. Construction of this P. pulmonarius genetic linkage map and identification of markers that are closely linked to the sporeless locus will facilitate marker-assisted selective breeding of a sporeless strain with economically important traits.


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